Partitioning-defective 3 Antibody | EMD Millipore 07-330 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Partitioning-defective 3 Antibody

catalog :

07-330

quantity :

200 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, chicken

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

citations: 22

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; human; fig 1c western blot; human; fig s8	Barnat M, Capizzi M, Aparicio E, Boluda S, Wennagel D, Kacher R, et al. Huntington's disease alters human neurodevelopment. Science. 2020;369:787-793 pubmed publisher
western blot; human; 1:1000; loading ...; fig 4a	Fearnley G, Young K, Edgar J, Antrobus R, Hay I, Liang W, et al. The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion. elife. 2019;8: pubmed publisher
western blot; human; 1:4000; loading ...; fig 6c immunocytochemistry; mouse; 1:100; loading ...; fig 5a	Jung H, Fattet L, Tsai J, Kajimoto T, Chang Q, Newton A, et al. Apical-basal polarity inhibits epithelial-mesenchymal transition and tumour metastasis by PAR-complex-mediated SNAI1 degradation. Nat Cell Biol. 2019;21:359-371 pubmed publisher
western blot; human; fig 2a	Hu L, Liang S, Chen H, Lv T, Wu J, Chen D, et al. Î”Np63Î± is a common inhibitory target in oncogenic PI3K/Ras/Her2-induced cell motility and tumor metastasis. Proc Natl Acad Sci U S A. 2017;114:E3964-E3973 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 1i western blot; mouse; loading ...; fig 1e	Mescher M, Jeong P, Knapp S, Rübsam M, Saynisch M, Kranen M, et al. The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma. J Exp Med. 2017;214:339-358 pubmed publisher
western blot; human; 1:400; loading ...; fig 5d	Mahale S, Kumar M, Sharma A, Babu A, Ranjan S, Sachidanandan C, et al. The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation. Sci Rep. 2016;6:22 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:100; loading ...	Yang Z, Zimmerman S, Tsunezumi J, Braitsch C, Trent C, Bryant D, et al. Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016;418:66-74 pubmed publisher
immunohistochemistry; mouse; loading ...; fig s1f	Porat Shliom N, Tietgens A, Van Itallie C, Vitale Cross L, Jarnik M, Harding O, et al. Liver kinase B1 regulates hepatocellular tight junction distribution and function in vivo. Hepatology. 2016;64:1317-29 pubmed publisher
immunocytochemistry; mouse; fig s2d	Rozo M, Li L, Fan C. Targeting ?1-integrin signaling enhances regeneration in aged and dystrophic muscle in mice. Nat Med. 2016;22:889-96 pubmed publisher
western blot; rat; 1:500; fig 5	Gao Y, Lui W, Lee W, Cheng C. Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells. Sci Rep. 2016;6:28589 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:50; fig 7 western blot; mouse; 1:500; fig 6	Campos Y, Qiu X, Gomero E, Wakefield R, Horner L, Brutkowski W, et al. Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun. 2016;7:11876 pubmed publisher
immunocytochemistry; dogs; fig s5	Klinkert K, Rocancourt M, Houdusse A, Echard A. Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening. Nat Commun. 2016;7:11166 pubmed publisher
immunohistochemistry; chicken; 1:500; fig 4	Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed publisher
immunohistochemistry; mouse; 1:250; fig 5	Jimeno D, Gómez C, Calzada N, de la Villa P, Lillo C, Santos E. RASGRF2 controls nuclear migration in postnatal retinal cone photoreceptors. J Cell Sci. 2016;129:729-42 pubmed publisher
	Wang Y, Feige P, Brun C, Hekmatnejad B, Dumont N, Renaud J, et al. EGFR-Aurka Signaling Rescues Polarity and Regeneration Defects in Dystrophin-Deficient Muscle Stem Cells by Increasing Asymmetric Divisions. Cell Stem Cell. 2019;24:419-432.e6 pubmed publisher
	Tenvooren I, Jenks M, Rashid H, Cook K, Muhlemann J, Sistrunk C, et al. Elevated leptin disrupts epithelial polarity and promotes premalignant alterations in the mammary gland. Oncogene. 2019;38:3855-3870 pubmed publisher
	Zulkipli I, Clark J, Hart M, Shrestha R, Gul P, Dang D, et al. Spindle rotation in human cells is reliant on a MARK2-mediated equatorial spindle-centering mechanism. J Cell Biol. 2018;217:3057-3070 pubmed publisher
	Halaoui R, Rejon C, Chatterjee S, Szymborski J, Meterissian S, Muller W, et al. Progressive polarity loss and luminal collapse disrupt tissue organization in carcinoma. Genes Dev. 2017;31:1573-1587 pubmed publisher
	Nakamura H, Nagasaka K, Kawana K, Taguchi A, Uehara Y, Yoshida M, et al. Expression of Par3 polarity protein correlates with poor prognosis in ovarian cancer. BMC Cancer. 2016;16:897 pubmed
	Jha H, Sun Z, Upadhyay S, El Naccache D, Singh R, Sahu S, et al. KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation. PLoS Pathog. 2016;12:e1005801 pubmed publisher
	Gan W, Zavortink M, Ludick C, Templin R, Webb R, Webb R, et al. Cell polarity defines three distinct domains in pancreatic Î²-cells. J Cell Sci. 2017;130:143-151 pubmed publisher
	Chow C, Ebine K, Knab L, Bentrem D, Kumar K, Munshi H. Cancer Cell Invasion in Three-dimensional Collagen Is Regulated Differentially by GÎ±13 Protein and Discoidin Domain Receptor 1-Par3 Protein Signaling. J Biol Chem. 2016;291:1605-18 pubmed publisher

product information

Catalog Number :

07-330

Subcategory :

Cell Structure

Product Name :

Anti-Partitioning-defective 3 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q8TEW0

Host Name :

Rabbit

Antigen :

Partitioning-defective 3

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-Partitioning-defective 3 Antibody

Cross Reactivity :

Human;Rat;Monkey;Mouse;Canine

Background :

PAR3 is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR3 interacts with PRCKI. PAR3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR3 interacts with F11R/JAM1. Antibodies against PAR3 are present in sera from patients with cutaneous T cell lymphomas.

ALT Names :

Atypical PKC isotype-specific-interacting protein;CTCL tumor antigen se2-5;atypical PKC isotype-specific interacting protein;par-3 (partitioning defective 3, C.elegans) homolog;par-3 partitioning defective 3 homolog (C. elegans);partitioning-defective protein 3 homolog

Immunogen :

GST fusion protein corresponding to residues 457-744 of mouse PAR-3 (accession number AY02657).

Specificity :

Recognizes all 3 slice isoforms of PAR-3, MW ~180, 150, 100 kDa.

Package Size :

200 μg

Uses :

Immunocytochemistry;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt.