This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
acetyl-Histone H4 (Lys16) Antibody
catalog :
07-329
quantity :
100 µL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, brewer's yeast
application :
western blot, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - free floating section, ChIP-Seq
citations: 36
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig s3a
Ryu K, Nandu T, Kim J, Challa S, DeBerardinis R, Kraus W. Metabolic regulation of transcription through compartmentalized NAD+ biosynthesis. Science. 2018;360: pubmed publisher
  • western blot; human; loading ...; fig s2a
Utani K, Fu H, Jang S, Marks A, Smith O, Zhang Y, et al. Phosphorylated SIRT1 associates with replication origins to prevent excess replication initiation and preserve genomic stability. Nucleic Acids Res. 2017;45:7807-7824 pubmed publisher
  • western blot; mouse
Raciti G, Spinelli R, Desiderio A, Longo M, Parrillo L, Nigro C, et al. Specific CpG hyper-methylation leads to Ankrd26 gene down-regulation in white adipose tissue of a mouse model of diet-induced obesity. Sci Rep. 2017;7:43526 pubmed publisher
  • western blot; human; loading ...; fig 7d
Clarke T, Sanchez Bailon M, Chiang K, Reynolds J, Herrero Ruiz J, Bandeiras T, et al. PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination. Mol Cell. 2017;65:900-916.e7 pubmed publisher
  • western blot; brewer's yeast; loading ...; fig s1d
Desfossés Baron K, Hammond Martel I, Simoneau A, Sellam A, Roberts S, Wurtele H. Valproate inhibits MAP kinase signalling and cell cycle progression in S. cerevisiae. Sci Rep. 2016;6:36013 pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig s2d
  • immunocytochemistry; mouse; loading ...; fig 3b
  • western blot; mouse; loading ...; fig 3a
  • immunocytochemistry; human; loading ...; fig 3b
  • western blot; human; loading ...; fig 3a
Zhong J, Li X, Cai W, Wang Y, Dong S, Yang J, et al. TET1 modulates H4K16 acetylation by controlling auto-acetylation of hMOF to affect gene regulation and DNA repair function. Nucleic Acids Res. 2017;45:672-684 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 8
  • western blot; mouse; loading ...; fig 8a
Liu Y, Wang T, Zhang R, Fu W, Wang X, Wang F, et al. Calorie restriction protects against experimental abdominal aortic aneurysms in mice. J Exp Med. 2016;213:2473-2488 pubmed
  • chromatin immunoprecipitation; human; loading ...; fig s4d
Wang D, Kon N, Lasso G, Jiang L, Leng W, Zhu W, et al. Acetylation-regulated interaction between p53 and SET reveals a widespread regulatory mode. Nature. 2016;538:118-122 pubmed publisher
  • chromatin immunoprecipitation; human; fig 2
Kim J, Yu J, Abdulkadir S, Chakravarti D. KAT8 Regulates Androgen Signaling in Prostate Cancer Cells. Mol Endocrinol. 2016;30:925-36 pubmed publisher
  • western blot; human; 1:1000; fig 3
Penterling C, Drexler G, Böhland C, Stamp R, Wilke C, Braselmann H, et al. Depletion of Histone Demethylase Jarid1A Resulting in Histone Hyperacetylation and Radiation Sensitivity Does Not Affect DNA Double-Strand Break Repair. PLoS ONE. 2016;11:e0156599 pubmed publisher
  • chromatin immunoprecipitation; human; fig s3
Brasa S, Mueller A, Jacquemont S, Hahne F, Rozenberg I, Peters T, et al. Reciprocal changes in DNA methylation and hydroxymethylation and a broad repressive epigenetic switch characterize FMR1 transcriptional silencing in fragile X syndrome. Clin Epigenetics. 2016;8:15 pubmed publisher
  • chromatin immunoprecipitation; brewer's yeast; loading ...; fig 1f
McCann T, Guo Y, McDonald W, Tansey W. Antagonistic roles for the ubiquitin ligase Asr1 and the ubiquitin-specific protease Ubp3 in subtelomeric gene silencing. Proc Natl Acad Sci U S A. 2016;113:1309-14 pubmed publisher
  • western blot; human; fig 3
Renaud E, Barascu A, Rosselli F. Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells. Nucleic Acids Res. 2016;44:648-56 pubmed publisher
  • immunohistochemistry - free floating section; mouse; fig 2
  • western blot; mouse; fig 1
Sheikh B, Bechtel Walz W, Lucci J, Karpiuk O, Hild I, Hartleben B, et al. MOF maintains transcriptional programs regulating cellular stress response. Oncogene. 2016;35:2698-710 pubmed publisher
Bonnefont J, Tiberi L, van den Ameele J, Potier D, Gaber Z, Lin X, et al. Cortical Neurogenesis Requires Bcl6-Mediated Transcriptional Repression of Multiple Self-Renewal-Promoting Extrinsic Pathways. Neuron. 2019;103:1096-1108.e4 pubmed publisher
Huang H, Kong W, Jean M, Fiches G, Zhou D, Hayashi T, et al. A CRISPR/Cas9 screen identifies the histone demethylase MINA53 as a novel HIV-1 latency-promoting gene (LPG). Nucleic Acids Res. 2019;: pubmed publisher
Urdinguio R, Lopez V, Bayon G, Díaz de la Guardia R, Sierra M, García Toraño E, et al. Chromatin regulation by Histone H4 acetylation at Lysine 16 during cell death and differentiation in the myeloid compartment. Nucleic Acids Res. 2019;47:5016-5037 pubmed publisher
Huai W, Liu X, Wang C, Zhang Y, Chen X, Chen X, et al. KAT8 selectively inhibits antiviral immunity by acetylating IRF3. J Exp Med. 2019;216:772-785 pubmed publisher
Bian C, Zhang C, Luo T, Vyas A, Chen S, Liu C, et al. NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression. Nat Commun. 2019;10:693 pubmed publisher
Grigoryan A, Guidi N, Senger K, Liehr T, Soller K, Marka G, et al. LaminA/C regulates epigenetic and chromatin architecture changes upon aging of hematopoietic stem cells. Genome Biol. 2018;19:189 pubmed publisher
Clouaire T, Rocher V, Lashgari A, Arnould C, Aguirrebengoa M, Biernacka A, et al. Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures. Mol Cell. 2018;72:250-262.e6 pubmed publisher
McCarthy A, Deiulio A, Martin E, Upadhyay M, Rangan P. Tip60 complex promotes expression of a differentiation factor to regulate germline differentiation in female Drosophila. Mol Biol Cell. 2018;29:2933-2945 pubmed publisher
Basilicata M, Bruel A, Semplicio G, Valsecchi C, Aktas T, Duffourd Y, et al. De novo mutations in MSL3 cause an X-linked syndrome marked by impaired histone H4 lysine 16 acetylation. Nat Genet. 2018;50:1442-1451 pubmed publisher
Valsecchi C, Basilicata M, Semplicio G, Georgiev P, Gutierrez N, Akhtar A. Facultative dosage compensation of developmental genes on autosomes in Drosophila and mouse embryonic stem cells. Nat Commun. 2018;9:3626 pubmed publisher
de Bruyn Kops A, Burke J, Guthrie C. Brr6 plays a role in gene recruitment and transcriptional regulation at the nuclear envelope. Mol Biol Cell. 2018;29:2578-2590 pubmed publisher
Saavedra F, Rivera C, Rivas E, Merino P, Garrido D, Hernandez S, et al. PP32 and SET/TAF-I? proteins regulate the acetylation of newly synthesized histone H4. Nucleic Acids Res. 2017;45:11700-11710 pubmed publisher
Fang J, Ianni A, Smolka C, Vakhrusheva O, Nolte H, Kruger M, et al. Sirt7 promotes adipogenesis in the mouse by inhibiting autocatalytic activation of Sirt1. Proc Natl Acad Sci U S A. 2017;114:E8352-E8361 pubmed publisher
Li L, Wang Y. Cross-talk between the H3K36me3 and H4K16ac histone epigenetic marks in DNA double-strand break repair. J Biol Chem. 2017;292:11951-11959 pubmed publisher
Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
Feldman S, Wuerffel R, Achour I, Wang L, Carpenter P, Kenter A. 53BP1 Contributes to Igh Locus Chromatin Topology during Class Switch Recombination. J Immunol. 2017;198:2434-2444 pubmed publisher
Klein B, Wang X, Cui G, Yuan C, Botuyan M, Lin K, et al. PHF20 Readers Link Methylation of Histone H3K4 and p53 with H4K16 Acetylation. Cell Rep. 2016;17:1158-1170 pubmed publisher
Roth M, Wang Z, Chen W. SIRT1 and LSD1 competitively regulate KU70 functions in DNA repair and mutation acquisition in cancer cells. Oncotarget. 2016;7:50195-50214 pubmed publisher
Saredi G, Huang H, Hammond C, Alabert C, Bekker Jensen S, Forne I, et al. H4K20me0 marks post-replicative chromatin and recruits the TONSL–MMS22L DNA repair complex. Nature. 2016;534:714-718 pubmed publisher
Menendez S, Villaronga M, Rodrigo J, Álvarez Teijeiro S, Urdinguio R, Fraga M, et al. HERG1A potassium channel is the predominant isoform in head and neck squamous cell carcinomas: evidence for regulation by epigenetic mechanisms. Sci Rep. 2016;6:19666 pubmed publisher
Mandal M, Hamel K, Maienschein Cline M, Tanaka A, Teng G, Tuteja J, et al. Histone reader BRWD1 targets and restricts recombination to the Igk locus. Nat Immunol. 2015;16:1094-103 pubmed publisher
Hait N, Wise L, Allegood J, O Brien M, Avni D, Reeves T, et al. Active, phosphorylated fingolimod inhibits histone deacetylases and facilitates fear extinction memory. Nat Neurosci. 2014;17:971-80 pubmed publisher
product information
Catalog Number :
07-329
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-acetyl-Histone H4 (Lys16) Antibody
Product Type :
Antibodies
Clonality :
Polyclonal Antibody
Gene ID :
P62805
Host Name :
Rabbit
Antigen :
acetyl-Histone H4 (Lys16)
Conjugate :
Affinity Purified
Isotype :
IgG
Product Description :
Anti-acetyl-Histone H4 (Lys16) Antibody
Cross Reactivity :
Human;Vertebrates;Yeast ( S. cerevisiae )
Background :
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
ALT Names :
H4K16Ac;Histone H4 (acetyl K16);H4 histone family, member A;histone 1, H4a;histone cluster 1, H4a
Immunogen :
Ovalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4.
Specificity :
Histone H4 acetylated on lysine 16, but not histone H4 containing a lysine 16 to arginine substitution.
Package Size :
100 µL
Uses :
Western Blotting;Multiplexing;Peptide Inhibition Assay;Dot Blot;ChIP-seq;Chromatin Immunoprecipitation (ChIP)
Storage :
Stable for 1 year at 2-8° C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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