dimethyl-Histone H3 (Lys36) Antibody | EMD Millipore 07-274 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

dimethyl-Histone H3 (Lys36) Antibody

catalog :

07-274

quantity :

200 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, chromatin immunoprecipitation, ChIP-Seq, other

citations: 4

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; human; loading ...; fig s7e	Li N, Xue W, Yuan H, Dong B, Ding Y, Liu Y, et al. AKT-mediated stabilization of histone methyltransferase WHSC1 promotes prostate cancer metastasis. J Clin Invest. 2017;127:1284-1302 pubmed publisher
other; human; 1:400; loading ...; fig 3	He Y, Selvaraju S, Curtin M, Jakob C, Zhu H, Comess K, et al. The EED protein-protein interaction inhibitor A-395 inactivates the PRC2 complex. Nat Chem Biol. 2017;13:389-395 pubmed publisher
	Gopalakrishnan R, Marr S, Kingston R, Winston F. A conserved genetic interaction between Spt6 and Set2 regulates H3K36 methylation. Nucleic Acids Res. 2019;47:3888-3903 pubmed publisher
	Leadem B, Kagiampakis I, Wilson C, Cheung T, Arnott D, Trojer P, et al. A KDM5 Inhibitor Increases Global H3K4 Trimethylation Occupancy and Enhances the Biological Efficacy of 5-Aza-2'-Deoxycytidine. Cancer Res. 2018;78:1127-1139 pubmed publisher

product information

Catalog Number :

07-274

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-dimethyl-Histone H3 (Lys36) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

dimethyl-Histone H3 (Lys36)

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-dimethyl-Histone H3 (Lys36) Antibody

Cross Reactivity :

Mouse;Human;Chicken;Drosophila;Vertebrates;Yeast ( S. cerevisiae )

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3K36me2;Histone H3 (di methyl K36);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

KLH-conjugated, synthetic peptide corresponding to amino acids 30-38 (CPATGGV(dimethyl-K)KP) of Histone H3 with a N-terminal C added for conjugation. The immunizing sequence is conserved from Tetrahymena to human.

Specificity :

Recognizes methylated Histone H3 (Lys36), Mr 17 kDa as determined by Immunoblotting. Does not cross react with peptides containing dimethyl-histone H4 Lys20, nor Histone H3 Lys4, Lys9, or Lys27.

Package Size :

200 µL

Uses :

Chromatin Immunoprecipitation (ChIP);Western Blotting;Dot Blot

Storage :

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.