phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325) | EMD Millipore 07-2273 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)

catalog :

07-2273

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, dogs

application :

western blot, immunohistochemistry - paraffin section

citations: 4

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - paraffin section; mouse; loading ...; fig 6b western blot; mouse; loading ...; fig 6d	Christensen I, Wu Q, Bohlbro A, Skals M, Damkier H, Hübner C, et al. Genetic disruption of slc4a10 alters the capacity for cellular metabolism and vectorial ion transport in the choroid plexus epithelium. Fluids Barriers CNS. 2020;17:2 pubmed publisher
	Volanakis A, Kamieniarz Gdula K, Schlackow M, Proudfoot N. WNK1 kinase and the termination factor PCF11 connect nuclear mRNA export with transcription. Genes Dev. 2017;31:2175-2185 pubmed publisher
	Jiang C, Kawabe H, Rotin D. The Ubiquitin Ligase Nedd4L Regulates the Na/K/2Cl Co-transporter NKCC1/SLC12A2 in the Colon. J Biol Chem. 2017;292:3137-3145 pubmed publisher
	Miraucourt L, Tsui J, Gobert D, Desjardins J, Schohl A, Sild M, et al. Endocannabinoid signaling enhances visual responses through modulation of intracellular chloride levels in retinal ganglion cells. elife. 2016;5: pubmed publisher

product information

Catalog Number :

07-2273

Subcategory :

Inflammation & Immunology

Product Name :

Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q9UEW8

Host Name :

Rabbit

Antigen :

phospho-SPAK (Ser373) / phospho-OSR1 (Ser325)

Conjugate :

Affinity Purified

Product Description :

Anti-phospho-SPAK Antibody (Ser373) / phospho-OSR1 Antibody (Ser325)

Cross Reactivity :

Human;Mouse;Rat;Canine

Background :

As members of the (sterile 20)-like (STE20) kinase family, the oxidative-stress-responsive kinase 1 (OSR1) and the SPS1-related proline/alanine-rich kinase (SPAK) serve as key enzymes in a signaling cascade, which mediate the activity of Na+/K+/2Cl- cotransporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which are 79% identical to each other at the sequence level, and are also highly conserved in orthologues of these enzymes in Caenorhabditis elegans, Drosophila, and Xenopus. The CCT domain reflects no similarity to sequences found in other proteins. This domain is known to recognize and interact with a unique peptide (Arg-Phe-Xaa-Val) motif, which is present in OSRT1- and SPAK-activating kinases (with-no-lysine-kinase 1 (WNK1 and WNK4) and also in STE20 kinase family substrates (NKCC1 and NKCC2). It is reported that phosphorylation of a Ser/Thr residue preceding the (Arg-Phe-Xaa-Val) motif results in steric clash, and subsequent dissociation from the CCT domain. Furthermore, patients suffering from Gordon syndrome carry mutated WNK1 and WNK4 genes.

ALT Names :

DCHT;Serine/threonine-protein kinase 39

Immunogen :

KLH-conjugated linear peptide corresponding to human SPAK and OSR1 phosphorylated at Ser375 and Ser325, respectively.

Specificity :

This antibody recognizes SPAK phosphorlyated at Ser373 and OSR1 phosphorlyated at Ser325.

Package Size :

100 μg

Uses :

Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt