Histone Macro H2A.1 Antibody | EMD Millipore 07-219 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Histone Macro H2A.1 Antibody

catalog :

07-219

quantity :

200 μL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, ChIP-Seq

citations: 5

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; human; loading ...; fig 7a immunohistochemistry - paraffin section; human; 1:50; fig 8i immunocytochemistry; human; 1:50; loading ...; fig 5c western blot; human; 1:1000; loading ...; fig 6a, 8a, s14a	Sang Y, Li Y, Zhang Y, Alvarez A, Yu B, Zhang W, et al. CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity. Nat Commun. 2019;10:4013 pubmed publisher
western blot; human; 1:1000; fig s1	Weyemi U, Redon C, Choudhuri R, Aziz T, Maeda D, Boufraqech M, et al. The histone variant H2A.X is a regulator of the epithelial-mesenchymal transition. Nat Commun. 2016;7:10711 pubmed publisher
chromatin immunoprecipitation; human; fig 5	Park S, Shim J, Park H, Eum D, Park M, Mi Yi J, et al. MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B. Oncogene. 2016;35:1292-301 pubmed publisher
	Clouaire T, Rocher V, Lashgari A, Arnould C, Aguirrebengoa M, Biernacka A, et al. Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures. Mol Cell. 2018;72:250-262.e6 pubmed publisher
	Kim J, Sturgill D, Sebastian R, Khurana S, Tran A, Edwards G, et al. Replication Stress Shapes a Protective Chromatin Environment across Fragile Genomic Regions. Mol Cell. 2018;69:36-47.e7 pubmed publisher

product information

Catalog Number :

07-219

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Histone Macro H2A.1 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q9QZQ8

Host Name :

Rabbit

Antigen :

Histone Macro H2A.1

Conjugate :

Affinity Purified

Product Description :

Anti-Histone Macro H2A.1 Antibody

Cross Reactivity :

Human;Mouse

Background :

Histone MacroH2A (mH2A) is a variant histone also known as H2AFY. mH2A is enriched in chromatin of the female inactive X chromosome and in highly methylated CpG loci. This histone variant is known to repress RNA polymerase II-driven transcription at the level of transcriptional initiation. In contrast to most histone variants containing only subtle changes, mH2A contains a large non-histone domain (the macro domain) on its C-terminus, resulting in a histone approximately three times the size of conventional H2A. There are 3 isoforms of mH2A, two of which (mH2A1.1, mH2A1.2 ) are formed by alternative splicing and another (mH2A2) from a separate gene. There are two phosphorylation sites within the basic hinge region of mH2A: Thr128 and Ser137. Phospho-Ser137 is enriched in mitosis, implicating mH2A in the regulation of posttranslational modifications throughout the cell cycle.

ALT Names :

H2A histone family, member Y;histone macroH2A1.2;macroH2A1.2;Histone H2A.y;Histone macroH2A;histone macroH2A1.1;Medulloblastoma antigen MU-MB-50.205;core histone macro-H2A.1

Immunogen :

GST fusion-protein corresponding to the nonhistone region of rat Histone macroH2A1 (residues 160-370).

Specificity :

This antibody recognizes Histone MacroH2A.1 at the macro domain.

Package Size :

200 μL

Uses :

Western Blotting;Immunocytochemistry;Immunohistochemistry

Storage :

Stable for 1 year at -20°C from date of receipt. Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.