phospho-H2A.X (Ser139) Antibody | EMD Millipore 07-164 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-H2A.X (Ser139) Antibody

catalog :

07-164

quantity :

200 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation

citations: 13

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:2000; loading ...; fig 1b	Martínez J, Tarallo D, Martinez Palma L, Victoria S, Bresque M, Rodriguez Bottero S, et al. Mitofusins modulate the increase in mitochondrial length, bioenergetics and secretory phenotype in therapy-induced senescent melanoma cells. Biochem J. 2019;476:2463-2486 pubmed publisher
immunocytochemistry; mouse; fig 3e	Li L, Guturi K, Gautreau B, Patel P, Saad A, Morii M, et al. Ubiquitin ligase RNF8 suppresses Notch signaling to regulate mammary development and tumorigenesis. J Clin Invest. 2018;128:4525-4542 pubmed publisher
western blot; human; loading ...; fig 1c	Bernal A, Moltó Abad M, Dominguez D, Tusell L. Acute telomere deprotection prevents ongoing BFB cycles and rampant instability in p16INK4a-deficient epithelial cells. Oncotarget. 2018;9:27151-27170 pubmed publisher
immunocytochemistry; human; loading ...; fig 2a western blot; human; loading ...; fig 3a	Tian Y, Shen X, Wang R, Klages Mundt N, Lynn E, Martin S, et al. Constitutive role of the Fanconi anemia D2 gene in the replication stress response. J Biol Chem. 2017;292:20184-20195 pubmed publisher
chromatin immunoprecipitation; human; fig 4e	Bleuyard J, Fournier M, Nakato R, Couturier A, Katou Y, Ralf C, et al. MRG15-mediated tethering of PALB2 to unperturbed chromatin protects active genes from genotoxic stress. Proc Natl Acad Sci U S A. 2017;114:7671-7676 pubmed publisher
immunocytochemistry; human; loading ...; fig 4e	Paul A, Wang B. RNF8- and Ube2S-Dependent Ubiquitin Lysine 11-Linkage Modification in Response to DNA Damage. Mol Cell. 2017;66:458-472.e5 pubmed publisher
immunocytochemistry; human; loading ...; fig 4a	Bhattacharya S, Srinivasan K, Abdisalaam S, Su F, Raj P, Dozmorov I, et al. RAD51 interconnects between DNA replication, DNA repair and immunity. Nucleic Acids Res. 2017;45:4590-4605 pubmed publisher
immunocytochemistry; human; 1:2000; loading ...; fig 1b	Marmisolle I, MartÃnez J, Liu J, Mastrogiovanni M, Fergusson M, Rovira I, et al. Reciprocal regulation of acetyl-CoA carboxylase 1 and senescence in human fibroblasts involves oxidant mediated p38 MAPK activation. Arch Biochem Biophys. 2017;613:12-22 pubmed publisher
immunocytochemistry; mouse; 1:500; fig 2	Ghamrasni S, Cardoso R, Li L, Guturi K, Bjerregaard V, Liu Y, et al. Rad54 and Mus81 cooperation promotes DNA damage repair and restrains chromosome missegregation. Oncogene. 2016;35:4836-45 pubmed publisher
immunocytochemistry; rat; 1:200	Grindheim A, Hollås H, Raddum A, Saraste J, Vedeler A. Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of annexin A2. J Cell Sci. 2016;129:314-28 pubmed publisher
	Bernal A, Zafon E, Dominguez D, Bertran E, Tusell L. Generation of Immortalised But Unstable Cells after hTERT Introduction in Telomere-Compromised and p53-Deficient vHMECs. Int J Mol Sci. 2018;19: pubmed publisher
	Yen W, Chaudhry A, Vaidyanathan B, Yewdell W, Pucella J, Sharma R, et al. BRCT-domain protein BRIT1 influences class switch recombination. Proc Natl Acad Sci U S A. 2017;: pubmed publisher
	Saquilabon Cruz G, Kong X, Silva B, Khatibzadeh N, Thai R, Berns M, et al. Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites. Nucleic Acids Res. 2016;44:e27 pubmed publisher

product information

Catalog Number :

07-164

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-H2A.X (Ser139) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P16104

Host Name :

Rabbit

Antigen :

phospho-H2A.X (Ser139)

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-phospho-H2A.X (Ser139) Antibody

Cross Reactivity :

Vertebrates;Human

Background :

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure.

ALT Names :

H2A histone family, member Q;histone 2, H2ac;histone IIa;histone cluster 2, H2ac

Immunogen :

KLH-conjugated, synthetic peptide (CKATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X. The immunizing sequence has 8 identical amino acids in yeast and mouse.

Specificity :

Histone H2A.X phosphorylated at Ser139.

Package Size :

200 µg

Uses :

Immunoprecipitation;Western Blotting;ChIP-seq

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.