EZH2 (Enhancer of Zeste Homologue 2) Antibody, internal | EMD Millipore 07-1583 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

EZH2 (Enhancer of Zeste Homologue 2) Antibody, internal

catalog :

07-1583

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:200; loading ...; fig 1a immunocytochemistry; human; 1:200; loading ...; fig 3h western blot; human; 1:200; loading ...; fig 3a	Lu W, Liu S, Li B, Xie Y, Izban M, Ballard B, et al. SKP2 loss destabilizes EZH2 by promoting TRAF6-mediated ubiquitination to suppress prostate cancer. Oncogene. 2017;36:1364-1373 pubmed publisher

product information

Catalog Number :

07-1583

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-EZH2 (Enhancer of Zeste Homologue 2) Antibody, internal

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q15910

Host Name :

Rabbit

Antigen :

EZH2 (Enhancer of Zeste Homologue 2)

Conjugate :

Purified

Product Description :

Anti-EZH2 (Enhancer of Zeste Homologue 2) Antibody, internal

Cross Reactivity :

Human;Mouse;Rat;Xenopus

Background :

Polycomb protein group complexes are responsible for the regulation of hundreds of genes in mammals and insects. These proteins are responsible for the assembly and packaging of chromatin and for the induction of methylation on Histone H3. EZH2 (enhancer of zeste homologue 2), also known as Enx1, is a member of the polycomb group of proteins and is involved in cell cycle regulation. EZH2 represses transcription via trimethylation of histone H3 on Lys27 (H3K27) as indicated by the fact that RNAi-mediated knockdown of EZH2 resulted in a loss of H3K27 trimethylation. The expression of this protein in human tumors is aggressive for hormone refractory prostate cancer. In breast cancer EZH2 expression was increased in malignant tumors and promoted anchorage independent and invasive growth in vitro. EZH2, also known as ENX-1, is ubiquitously expressed during early embryo genesis, and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, EZH2 is restricted to the spleen, testis and placenta. EZH2 and BMI-1 genes are co expressed in Reed-Sternberg cells of Hodgkin’s disease. Coexpression of BMI-1 and EZH2 is also associated with cycling cells and degree of malignancy in B-cell non-Hogkin’s lymphoma. EZH2 is involved in the progression of prostate cancer, and has been identified as a marker of aggressive breast cancer. Two transcript variants encoding distinct isoforms have been identified for this gene.

ALT Names :

Enhancer of zeste homolog 2;Lysine N-methyltransferase 6 ;enhancer of zeste 2;enhancer of zeste homolog 2 ;ENX-1

Immunogen :

Immunogen was a KLH-conjugated synthetic peptide corresponding to a.a. 260-277 (CTPNIDGPNAKSVQREQS) of human EHZ2 (similar to enhancer of zeste (Drosophila) homolog 2).

Specificity :

Recognizes EZH2, Mr ~100 kDa.

Package Size :

100 µL

Uses :

Western Blotting;ELISA

Storage :

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: To maximize recovery, centrifuge briefly prior to removing the cap. Avoid freeze/thawing the product, which can damage IgG and affect product performance.