IκBα Antibody | EMD Millipore 07-1483 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

IκBα Antibody

catalog :

07-1483

quantity :

100 μL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunoprecipitation, immunohistochemistry - paraffin section

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:4000; loading ...; fig 4a	Kalita B, Ranjan R, Singh A, Yashavarddhan M, Bajaj S, Gupta M. A Combination of Podophyllotoxin and Rutin Attenuates Radiation Induced Gastrointestinal Injury by Negatively Regulating NF-?B/p53 Signaling in Lethally Irradiated Mice. PLoS ONE. 2016;11:e0168525 pubmed publisher

product information

Catalog Number :

07-1483

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-IκBα Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P25963

Host Name :

Rabbit

Antigen :

IκBα

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-IκBα Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

IKK beta (IκB Kinase, IKKβ, I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. IκB Kinase (IKK) complex is a critical component in NFκB regulation. In a majority of unstimulated cells, the NFκB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IκB proteins. For NFκB to become activated, it must first disassociate from the inhibitor IκB. This occurs via the serine phosphorylation of IκB on Ser32 and Ser36. Post receptor stimulation, most notably by TNFα, NIK, a serine/threonine kinase, activates and phosphorylates IKKα and IKKβ. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates, which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1. IKK then binds to and phosphorylates IκBα on the two serine residues. This phosphorylation marks IκB for ubiquination and destruction by the proteosome. This frees the NFκB complex and allows it to translocate to the nucleus.

Immunogen :

IκBα peptide corresponding to a region near the C-terminus of the human protein, conjugated to KLH.

Specificity :

Recognizes IκBα, C-terminus. May react non-specifically with other proteins. Control peptide provided will compete only with the specific reaction of antiserum with IκBα.

Package Size :

100 μL

Uses :

Western Blotting;Immunohistochemistry (Paraffin);ELISA;Immunoprecipitation

Storage :

Both antibody and control are stable for 6 months at -20ºC in undiluted aliquots from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.