VEGF Antibody | EMD Millipore 07-1420 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

VEGF Antibody

catalog :

07-1420

quantity :

50 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section, blocking or activating experiments

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 5g	Wu Q, Ma Y, Ruan C, Yang Y, Liu X, Ge Q, et al. Loss of osteoglycin promotes angiogenesis in limb ischaemia mouse models via modulation of vascular endothelial growth factor and vascular endothelial growth factor receptor 2 signalling pathway. Cardiovasc Res. 2017;113:70-80 pubmed publisher
blocking or activating experiments; human; fig 6	Zhao C, Zhang W, Zhao Y, Yang Y, Luo H, Ji G, et al. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism. Sci Rep. 2016;6:19404 pubmed publisher
	Deng Z, Zhou L, Wang Y, Liao S, Huang Y, Shan Y, et al. Astrocyte-derived VEGF increases cerebral microvascular permeability under high salt conditions. Aging (Albany NY). 2020;12:11781-11793 pubmed publisher
	Tabola R, Augoff K, Lewandowski A, Ziolkowski P, Szelachowski P, Grabowski K. Esophageal anastomosis - how the granulation phase of wound healing improves the incidence of anastomotic leakage. Oncol Lett. 2016;12:2038-2044 pubmed

product information

Catalog Number :

07-1420

Subcategory :

Cell Signaling

Product Name :

Anti-VEGF Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P15692

Host Name :

Rabbit

Antigen :

VEGF

Conjugate :

Affinity Purified

Isotype :

IgG

Product Description :

Anti-VEGF Antibody

Cross Reactivity :

Human;Mouse

Background :

VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. VEGF induces angiogenesis as well as permeabilization of blood vessels, and plays a central role in the regulation of vasculogenesis. VEGF expression correlates to the degree of tumor vascularization and with increased metastatic risk. Additionally, VEGF induces endothelial cell proliferation, promotes cell migration, and inhibits apoptosis. Consequently, inhibition of VEGF signaling abrogates the development of a wide variety of tumors.

ALT Names :

Vascular permeability factor

Immunogen :

Highly pure ( 98%) recombinant mouse Vascular Endothelial Growth Factor.

Specificity :

Recognizes Vascular Endothelial Growth Factor (VEGF), a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability.

Package Size :

50 μg

Uses :

Western Blotting;Immunohistochemistry (Paraffin);ELISA

Storage :

Reconstituted antibody, at a concentration of ≥ 0.2 mg/mL, is stable for at least six weeks at 2-8ºC. Aliquots stored at -20ºC are stable for at least one year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.