GLUT-1 Antibody, CT | EMD Millipore 07-1401 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

GLUT-1 Antibody, CT

catalog :

07-1401

quantity :

50 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 19

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 3a	Contat C, Ancey P, Zangger N, Sabatino S, Pascual J, Escrig S, et al. Combined deletion of Glut1 and Glut3 impairs lung adenocarcinoma growth. elife. 2020;9: pubmed publisher
western blot; human; 1:500; loading ...; fig 4d, 6a, 6f, s6	Ghezzi C, Wong A, Chen B, Ribalet B, Damoiseaux R, Clark P. A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells. Nat Commun. 2019;10:5444 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig 1i	Diéguez Hurtado R, Kato K, Giaimo B, Nieminen Kelhä M, Arf H, Ferrante F, et al. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes. Nat Commun. 2019;10:2817 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 4b	Shah A, Melhuish T, Fox T, Frierson H, Wotton D. TGIF transcription factors repress acetyl CoA metabolic gene expression and promote intestinal tumor growth. Genes Dev. 2019;33:388-402 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; loading ...	Gerber D, Ghidinelli M, Tinelli E, Somandin C, Gerber J, Pereira J, et al. Schwann cells, but not Oligodendrocytes, Depend Strictly on Dynamin 2 Function. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig s5d	Segarra M, Aburto M, Cop F, Llaó Cid C, Härtl R, Damm M, et al. Endothelial Dab1 signaling orchestrates neuro-glia-vessel communication in the central nervous system. Science. 2018;361: pubmed publisher
western blot; human; 1:1000; fig s4e	Chhipa R, Fan Q, Anderson J, Muraleedharan R, Huang Y, Ciraolo G, et al. AMP kinase promotes glioblastoma bioenergetics and tumour growth. Nat Cell Biol. 2018;20:823-835 pubmed publisher
western blot; human; 1:1000; loading ...; fig 1e	Mai W, Gosa L, Daniëls V, Ta L, Tsang J, Higgins B, et al. Cytoplasmic p53 couples oncogene-driven glucose metabolism to apoptosis and is a therapeutic target in glioblastoma. Nat Med. 2017;23:1342-1351 pubmed publisher
immunohistochemistry; mouse; loading ...; fig 6f	Kim J, Kim Y, Kim J, Park D, Bae H, Lee D, et al. YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation. J Clin Invest. 2017;127:3441-3461 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:400; loading ...; fig s4a	Yanagida K, Liu C, Faraco G, Galvani S, Smith H, Burg N, et al. Size-selective opening of the blood-brain barrier by targeting endothelial sphingosine 1-phosphate receptor 1. Proc Natl Acad Sci U S A. 2017;114:4531-4536 pubmed publisher
western blot; human; 1:10,000; loading ...; fig 4d	Azzalin A, Nato G, Parmigiani E, Garello F, Buffo A, Magrassi L. Inhibitors of GLUT/SLC2A Enhance the Action of BCNU and Temozolomide against High-Grade Gliomas. Neoplasia. 2017;19:364-373 pubmed publisher
immunocytochemistry; mouse; 1:100; fig 3	Busse B, Bezrukov L, Blank P, Zimmerberg J. Resin embedded multicycle imaging (REMI): a tool to evaluate protein domains. Sci Rep. 2016;6:30284 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...	Johansson E, RÃ¶nÃ¶ B, Johansson M, Lindgren D, MÃ¶ller C, Axelson H, et al. Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice. Sci Rep. 2016;6:30739 pubmed publisher
western blot; human; fig 3	Hansen N, Hjort L, Broholm C, Gillberg L, SchrÃ¶lkamp M, Schultz H, et al. Metabolic and Transcriptional Changes in Cultured Muscle Stem Cells from Low Birth Weight Subjects. J Clin Endocrinol Metab. 2016;101:2254-64 pubmed publisher
western blot; mouse; fig 1	Peteranderl C, Morales Nebreda L, Selvakumar B, Lecuona E, VadÃ¡sz I, Morty R, et al. Macrophage-epithelial paracrine crosstalk inhibits lung edema clearance during influenza infection. J Clin Invest. 2016;126:1566-80 pubmed publisher
western blot; human; fig 1	Ruf M, Moch H, Schraml P. PD-L1 expression is regulated by hypoxia inducible factor in clear cell renal cell carcinoma. Int J Cancer. 2016;139:396-403 pubmed publisher
immunohistochemistry; mouse; 1:100; fig s6	Van Woensel M, Wauthoz N, RosiÃ¨re R, Mathieu V, Kiss R, Lefranc F, et al. Development of siRNA-loaded chitosan nanoparticles targeting Galectin-1 for the treatment of glioblastoma multiforme via intranasal administration. J Control Release. 2016;227:71-81 pubmed publisher
	Travisano S, Oliveira V, Prados B, Grego Bessa J, Piñeiro Sabarís R, Bou V, et al. Coronary arterial development is regulated by a Dll4-Jag1-EphrinB2 signaling cascade. elife. 2019;8: pubmed publisher
	White S, Avantaggiati M, Nemazanyy I, Di Poto C, Yang Y, Pende M, et al. YAP/TAZ Inhibition Induces Metabolic and Signaling Rewiring Resulting in Targetable Vulnerabilities in NF2-Deficient Tumor Cells. Dev Cell. 2019;49:425-443.e9 pubmed publisher

product information

Catalog Number :

07-1401

Subcategory :

Cell Signaling

Product Name :

Anti-GLUT-1 Antibody, CT

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P11166

Host Name :

Rabbit

Antigen :

GLUT-1

Conjugate :

Affinity Purified

Isotype :

IgG

Product Description :

Anti-GLUT-1 Antibody, CT

Cross Reactivity :

Human;Mouse;Rat

Background :

GLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT-1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55kDa and a 45 kDa. The 55kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake. GLUT2 is a facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. GLUT-4 is present in skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species. The expression of GLUT-4 is greatly influenced by insulin and other stimulators of the IP3 signaling pathway. Binding of insulin to the insulin receptor signals through PI3K and Akt to translocate GLUT-4 to the plasma membrane where it allows the uptake of glucose.

ALT Names :

Glucose Transporter type 1;Solute carrier family 2, facilitated glucose transporter, member 1

Immunogen :

Synthetic peptide corresponding amino acids with the C-terminus of human GLUT-1 coupled to KLH (C-ELFHPLGADSQV).

Specificity :

This antibody recognizes GLUT-1.

Package Size :

50 μg

Uses :

Western Blotting;Immunohistochemistry (Paraffin);Immunocytochemistry;ELISA

Storage :

Stable for 1 year at 2-8°C from date of receipt