Sirt1(Sir2) Antibody | EMD Millipore 07-131 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Sirt1(Sir2) Antibody

catalog :

07-131

quantity :

200 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, western blot knockout validation

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:500; fig 5	Heyward F, Gilliam D, Coleman M, Gavin C, Wang J, Kaas G, et al. Obesity Weighs down Memory through a Mechanism Involving the Neuroepigenetic Dysregulation of Sirt1. J Neurosci. 2016;36:1324-35 pubmed publisher
western blot; mouse; loading ...; fig 5e	Koronowski K, Kinouchi K, Welz P, Smith J, Zinna V, Shi J, et al. Defining the Independence of the Liver Circadian Clock. Cell. 2019;177:1448-1462.e14 pubmed publisher
western blot; human; loading ...; fig s3b	Utani K, Fu H, Jang S, Marks A, Smith O, Zhang Y, et al. Phosphorylated SIRT1 associates with replication origins to prevent excess replication initiation and preserve genomic stability. Nucleic Acids Res. 2017;45:7807-7824 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig 3d	Yuan X, Qi H, Li X, Wu F, Fang J, Bober E, et al. Disruption of spatiotemporal hypoxic signaling causes congenital heart disease in mice. J Clin Invest. 2017;127:2235-2248 pubmed publisher
immunoprecipitation; mouse; fig 5d western blot; mouse; fig 5d	Hui X, Zhang M, Gu P, Li K, Gao Y, Wu D, et al. Adipocyte SIRT1 controls systemic insulin sensitivity by modulating macrophages in adipose tissue. EMBO Rep. 2017;18:645-657 pubmed publisher
western blot; mouse; 1:5000; loading ...; fig 2k	Nakazawa H, Chang K, Shinozaki S, Yasukawa T, Ishimaru K, Yasuhara S, et al. iNOS as a Driver of Inflammation and Apoptosis in Mouse Skeletal Muscle after Burn Injury: Possible Involvement of Sirt1 S-Nitrosylation-Mediated Acetylation of p65 NF-κB and p53. PLoS ONE. 2017;12:e0170391 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig 8 immunocytochemistry; mouse; loading ...; fig 4c western blot; mouse; loading ...; fig 8f	Liu Y, Wang T, Zhang R, Fu W, Wang X, Wang F, et al. Calorie restriction protects against experimental abdominal aortic aneurysms in mice. J Exp Med. 2016;213:2473-2488 pubmed
immunoprecipitation; mouse; loading ...; fig 6b western blot; mouse; loading ...; fig 6b	Gallardo Montejano V, Saxena G, Kusminski C, Yang C, McAfee J, Hahner L, et al. Nuclear Perilipin 5 integrates lipid droplet lipolysis with PGC-1?/SIRT1-dependent transcriptional regulation of mitochondrial function. Nat Commun. 2016;7:12723 pubmed publisher
western blot; human; 1:5000; loading ...; fig 5a	Matsui M, Prakash T, Corey D. Argonaute 2-dependent Regulation of Gene Expression by Single-stranded miRNA Mimics. Mol Ther. 2016;24:946-55 pubmed publisher
western blot; mouse; 1:200; fig 1d	Kondo H, Kim H, Wang L, Okada M, Paul C, Millard R, et al. Blockade of senescence-associated microRNA-195 in aged skeletal muscle cells facilitates reprogramming to produce induced pluripotent stem cells. Aging Cell. 2016;15:56-66 pubmed publisher
	Choi S, Kwon S, Seok S, Xiao Z, Lee K, Kang Y, et al. Obesity-Linked Phosphorylation of SIRT1 by Casein Kinase 2 Inhibits Its Nuclear Localization and Promotes Fatty Liver. Mol Cell Biol. 2017;37: pubmed publisher
	Pozo M, Rodriguez Rodriguez R, Ramirez S, Seoane Collazo P, Lopez M, Serra D, et al. Hypothalamic Regulation of Liver and Muscle Nutrient Partitioning by Brain-Specific Carnitine Palmitoyltransferase 1C in Male Mice. Endocrinology. 2017;158:2226-2238 pubmed publisher
	Das D, Smith N, Wang X, Morgan I. The Deacetylase SIRT1 Regulates the Replication Properties of Human Papillomavirus 16 E1 and E2. J Virol. 2017;91: pubmed publisher
	Clark Knowles K, He X, Jardine K, Coulombe J, Dewar Darch D, Caron A, et al. Reversible modulation of SIRT1 activity in a mouse strain. PLoS ONE. 2017;12:e0173002 pubmed publisher
	Pantazi E, Folch Puy E, Bejaoui M, Panisello A, Varela A, Rolo A, et al. PPARÎ± Agonist WY-14643 Induces SIRT1 Activity in Rat Fatty Liver Ischemia-Reperfusion Injury. Biomed Res Int. 2015;2015:894679 pubmed publisher
	Tran D, Bergholz J, Zhang H, He H, Wang Y, Zhang Y, et al. Insulin-like growth factor-1 regulates the SIRT1-p53 pathway in cellular senescence. Aging Cell. 2014;13:669-78 pubmed publisher

product information

Catalog Number :

07-131

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Sirt1(Sir2) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q923E4

Host Name :

Rabbit

Antigen :

Sirt1(Sir2)

Conjugate :

Affinity Purified

Isotype :

IgG

Product Description :

Anti-Sirt1(Sir2) Antibody

Cross Reactivity :

Mouse;Human

Background :

Sirtuins are a family of closely related enzymes named after the yeast gene Sir2 (silent mating type information regulation 2). Members of the Sirtuin family have been found in nearly all organisms studied. Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Sirtuins have been implicated in the regulation of aging, transcription, apoptosis and stress resistance. Sirt1 (also known as Sirtuin 1, Sir2L1, and Sir2a), the mammalian homolog of yeast Sir2, has been shown to deacetylate and thereby deactivate the p53 protein to regulate cellular processes.

ALT Names :

SIR2-like protein 2;silencing information regulator 2-like;silent information regulator 2;sir2-related protein type 2;sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae);sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 2;sirtuin 2;sirtuin type 2

Immunogen :

GST-tagged fusion protein corresponding to amino acids 1-131 of mouse Sir2α.

Specificity :

This antibody recognizes Sirt1.

Package Size :

200 µL

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.