Notch 2 Antibody, intracellular | EMD Millipore 07-1233 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Notch 2 Antibody, intracellular

catalog :

07-1233

quantity :

200 μL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

product information

Catalog Number :

07-1233

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Notch 2 Antibody, intracellular

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q04721

Host Name :

Rabbit

Antigen :

Notch 2

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-Notch 2 Antibody, intracellular

Cross Reactivity :

Human;Mouse

Background :

Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.

ALT Names :

Neurogenic locus notch homolog protein 2 precursor;Notch (Drosophila) homolog;Notch 2;Notch homolog 2 (Drosophila)

Immunogen :

Synthetic peptide from amino acids 2396-2409 of human Notch 2.

Specificity :

Notch 2, intracellular. The antibody is also expected to react with rat and mouse due to immunogen sequence homology (13/14).

Package Size :

200 μL

Uses :

ELISA;Western Blotting;Immunohistochemistry (Paraffin)

Storage :

Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.