Notch 1 Antibody, NT | EMD Millipore 07-1232 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Notch 1 Antibody, NT

catalog :

07-1232

quantity :

200 μL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunohistochemistry, immunohistochemistry - paraffin section

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:200; loading ...; fig 2e	Guo Q, Xu H, Yang X, Zhao D, Liu S, Sun X, et al. Notch activation of Ca2+-sensing receptor mediates hypoxia-induced pulmonary hypertension. Hypertens Res. 2017;40:117-129 pubmed publisher

product information

Catalog Number :

07-1232

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Notch 1 Antibody, NT

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P46531

Host Name :

Rabbit

Antigen :

Notch 1

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-Notch 1 Antibody, NT

Cross Reactivity :

Human;Mouse

Background :

Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development.

ALT Names :

Neurogenic locus notch homolog protein 1 precursor;Notch (Drosophila) homolog 1 (translocation-associated);Notch 1;Notch homolog 1, translocation-associated (Drosophila);Translocation-associated notch protein TAN-1;Neurogenic locus notch homolog protein 1

Immunogen :

Synthetic peptide from the N-terminal sequence of the cleaved N intracellular domain (NICD) human Notch 1.

Specificity :

Notch 1, cleaved N terminal. Only the cleaved intracellular (activated) form is detected. No reactivity is detected against mouse N2, N3 or N4. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form.

Package Size :

200 μL

Uses :

ELISA;Western Blotting;Immunohistochemistry (Paraffin)

Storage :

Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.