phospho-Upf1 (Ser1127) Antibody | EMD Millipore 07-1016 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Upf1 (Ser1127) Antibody

catalog :

07-1016

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, chicken

application :

western blot, immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig 1b	Mooney C, Jimenez Mateos E, Engel T, Mooney C, Diviney M, Venø M, et al. RNA sequencing of synaptic and cytoplasmic Upf1-bound transcripts supports contribution of nonsense-mediated decay to epileptogenesis. Sci Rep. 2017;7:41517 pubmed publisher
western blot; human; 1:1000; fig s5	Durand S, Franks T, Lykke Andersen J. Hyperphosphorylation amplifies UPF1 activity to resolve stalls in nonsense-mediated mRNA decay. Nat Commun. 2016;7:12434 pubmed publisher

product information

Catalog Number :

07-1016

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Upf1 (Ser1127) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q92900

Host Name :

Rabbit

Antigen :

phospho-Upf1 (Ser1127)

Conjugate :

Affinity Purified

Product Description :

Anti-phospho-Upf1 (Ser1127) Antibody

Cross Reactivity :

Human;Xenopus;Bovine;Mouse;Rat;Chicken

Background :

Upf1 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. In mammalian cells NMD generally requires that translation terminates upstream of a post-splicing exon junction complex (EJC). The subsequent binding of Upf1 to the EJC triggers Upf1 phosphorylation. Data suggests that phospho-Upf1 interacts directly with eIF3 and inhibits the eIF3-dependent conversion of 40S ribosome complex to translationally competent 80S ribosome initiation complex to repress continued translation initiation.

ALT Names :

Regulator of nonsense transcripts 1;ATP-dependent helicase RENT1;Nonsense mRNA reducing factor 1;NORF1;Up-frameshift suppressor 1 homolog;hUpf1

Immunogen :

KLH-conjugated linear peptide corresponding to Upf1 phosphorylated at Ser1127.

Specificity :

This antibody recognizes Upf1 phosphorylated at Ser1127.

Package Size :

100 μg

Uses :

Western Blotting;Immunoprecipitation

Storage :

Stable for 1 year at 2-8°C from date of receipt