phospho (Ser10)-acetyl (Lys14)-Histone H3 Antibody | EMD Millipore 07-081 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho (Ser10)-acetyl (Lys14)-Histone H3 Antibody

catalog :

07-081

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, chromatin immunoprecipitation

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; fig 5	Badal S, Wang Y, Long J, Corcoran D, CHANG B, Truong L, et al. miR-93 regulates Msk2-mediated chromatin remodelling in diabetic nephropathy. Nat Commun. 2016;7:12076 pubmed publisher
	Solagna F, Nogara L, Dyar K, Greulich F, Mir A, Türk C, et al. Exercise-dependent increases in protein synthesis are accompanied by chromatin modifications and increased MRTF-SRF signalling. Acta Physiol (Oxf). 2020;:e13496 pubmed publisher
	Wang S, Ko S, Kim Y, Jo S, Lee S, Jung S, et al. Capsaicin upregulates HDAC2 via TRPV1 and impairs neuronal maturation in mice. Exp Mol Med. 2018;50:e455 pubmed publisher
	Papale A, Morella I, Indrigo M, Bernardi R, Marrone L, Marchisella F, et al. Impairment of cocaine-mediated behaviours in mice by clinically relevant Ras-ERK inhibitors. elife. 2016;5: pubmed publisher
	Mandal M, Hamel K, Maienschein Cline M, Tanaka A, Teng G, Tuteja J, et al. Histone reader BRWD1 targets and restricts recombination to the Igk locus. Nat Immunol. 2015;16:1094-103 pubmed publisher

product information

Catalog Number :

07-081

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

phospho (Ser10)-acetyl (Lys14)-Histone H3

Conjugate :

Affinity Purified

Isotype :

IgG

Product Description :

Anti-phospho (Ser10)-acetyl (Lys14)-Histone H3 Antibody

Cross Reactivity :

Mouse;Human;Eukaryote

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3K14me3S10P;Histone H3 (tri methyl K14, phospho S10);H3 histone family, member T;histone 3, H3;histone cluster 3, H3

Immunogen :

KLH-conjugated, synthetic peptide corresponding to amino acids 7-20 of human histone H3 (ARK[pS]TGGAcKAPRKQL-C).

Specificity :

Recognizes Histone H3 phosphorylated at serine 10 and acetylated at lysine 14, Mr 17 kDa. An unidentified protein is also seen in some cells lines, Mr 40 kDa.

Package Size :

100 µL

Uses :

Immunocytochemistry;Western Blotting;ChIP-seq;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.