This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
dimethyl-Histone H3 (Lys4) Antibody
catalog :
07-030
quantity :
200 µL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, fruit fly , roundworm
application :
western blot, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq, other
citations: 43
Published Application/Species/Sample/DilutionReference
  • ChIP-Seq; fruit fly ; loading ...; fig 1s2h
Fabry M, Ciabrelli F, Munafò M, Eastwood E, Kneuss E, Falciatori I, et al. piRNA-guided co-transcriptional silencing coopts nuclear export factors. elife. 2019;8: pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig s3e
Benchetrit H, Jaber M, Zayat V, Sebban S, Pushett A, Makedonski K, et al. Direct Induction of the Three Pre-implantation Blastocyst Cell Types from Fibroblasts. Cell Stem Cell. 2019;24:983-994.e7 pubmed publisher
  • other; human; loading ...; fig 4c
Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  • western blot; mouse; fig 1f
Hoshii T, Cifani P, Feng Z, Huang C, Koche R, Chen C, et al. A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response. Cell. 2018;172:1007-1021.e17 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig s9e
  • chromatin immunoprecipitation; human; loading ...; fig s9a
Gosselin D, Skola D, Coufal N, Holtman I, Schlachetzki J, Sajti E, et al. An environment-dependent transcriptional network specifies human microglia identity. Science. 2017;356: pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig s3e
Busslinger G, Stocsits R, van der Lelij P, Axelsson E, Tedeschi A, Galjart N, et al. Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl. Nature. 2017;544:503-507 pubmed publisher
  • chromatin immunoprecipitation; rat; loading ...; tbl 1
Kumar A, Dumasia K, Deshpande S, Balasinor N. Direct regulation of genes involved in sperm release by estrogen and androgen through their receptors and coregulators. J Steroid Biochem Mol Biol. 2017;171:66-74 pubmed publisher
  • western blot; roundworm ; 1:2000; fig s5a
Amendola P, Zaghet N, Ramalho J, Vilstrup Johansen J, Boxem M, Salcini A. JMJD-5/KDM8 regulates H3K36me2 and is required for late steps of homologous recombination and genome integrity. PLoS Genet. 2017;13:e1006632 pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig 1c
Hu Y, Zhang Z, Kashiwagi M, Yoshida T, Joshi I, Jena N, et al. Superenhancer reprogramming drives a B-cell-epithelial transition and high-risk leukemia. Genes Dev. 2016;30:1971-90 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 4c
  • western blot; mouse; loading ...; fig 4f
Patrick N, Griggs C, Icenogle A, Gilpatrick M, Kadiyala V, Jaime Frias R, et al. Class I lysine deacetylases promote glucocorticoid-induced transcriptional repression through functional interaction with LSD1. J Steroid Biochem Mol Biol. 2017;167:1-13 pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig 3c
Zeng X, Jedrychowski M, Chen Y, Serag S, Lavery G, Gygi S, et al. Lysine-specific demethylase 1 promotes brown adipose tissue thermogenesis via repressing glucocorticoid activation. Genes Dev. 2016;30:1822-36 pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig 5a
Allison K, Sajti E, Collier J, Gosselin D, Troutman T, Stone E, et al. Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells. elife. 2016;5: pubmed publisher
  • chromatin immunoprecipitation; human; fig 1
Castaño J, Morera C, Sesé B, Boue S, Bonet Costa C, Marti M, et al. SETD7 Regulates the Differentiation of Human Embryonic Stem Cells. PLoS ONE. 2016;11:e0149502 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 2
Liu Y, Duong W, Krawczyk C, Bretschneider N, Borbély G, Varshney M, et al. Oestrogen receptor β regulates epigenetic patterns at specific genomic loci through interaction with thymine DNA glycosylase. Epigenetics Chromatin. 2016;9:7 pubmed publisher
  • chromatin immunoprecipitation; human; fig 4
Brasa S, Mueller A, Jacquemont S, Hahne F, Rozenberg I, Peters T, et al. Reciprocal changes in DNA methylation and hydroxymethylation and a broad repressive epigenetic switch characterize FMR1 transcriptional silencing in fragile X syndrome. Clin Epigenetics. 2016;8:15 pubmed publisher
  • immunocytochemistry; mouse; 1:500; fig s3
Amouroux R, Nashun B, Shirane K, Nakagawa S, Hill P, D Souza Z, et al. De novo DNA methylation drives 5hmC accumulation in mouse zygotes. Nat Cell Biol. 2016;18:225-233 pubmed publisher
Feurtey A, Lorrain C, Croll D, Eschenbrenner C, Freitag M, Habig M, et al. Genome compartmentalization predates species divergence in the plant pathogen genus Zymoseptoria. BMC Genomics. 2020;21:588 pubmed publisher
Catizone A, Uzunbas G, Celadova P, Kuang S, Bose D, Sammons M. Locally acting transcription factors regulate p53-dependent cis-regulatory element activity. Nucleic Acids Res. 2020;48:4195-4213 pubmed publisher
Li Y, Liao Z, Luo H, Benyoucef A, Kang Y, Lai Q, et al. Alteration of CTCF-associated chromatin neighborhood inhibits TAL1-driven oncogenic transcription program and leukemogenesis. Nucleic Acids Res. 2020;48:3119-3133 pubmed publisher
Zhang H, Khoa L, Mao F, Xu H, Zhou B, Han Y, et al. MLL1 Inhibition and Vitamin D Signaling Cooperate to Facilitate the Expanded Pluripotency State. Cell Rep. 2019;29:2659-2671.e6 pubmed publisher
Anastas J, Zee B, Kalin J, Kim M, Guo R, Alexandrescu S, et al. Re-programing Chromatin with a Bifunctional LSD1/HDAC Inhibitor Induces Therapeutic Differentiation in DIPG. Cancer Cell. 2019;36:528-544.e10 pubmed publisher
Zoeller E, PEDRO B, Konen J, Dwivedi B, Rupji M, Sundararaman N, et al. Genetic heterogeneity within collective invasion packs drives leader and follower cell phenotypes. J Cell Sci. 2019;132: pubmed publisher
Fiorucci A, Bourbousse C, Concia L, Rougée M, Deton Cabanillas A, Zabulon G, et al. Arabidopsis S2Lb links AtCOMPASS-like and SDG2 activity in H3K4me3 independently from histone H2B monoubiquitination. Genome Biol. 2019;20:100 pubmed publisher
Yang C, Shen W, Chen H, Chu L, Xu Y, Zhou X, et al. Characterization and subcellular localization of histone deacetylases and their roles in response to abiotic stresses in soybean. BMC Plant Biol. 2018;18:226 pubmed publisher
Clouaire T, Rocher V, Lashgari A, Arnould C, Aguirrebengoa M, Biernacka A, et al. Comprehensive Mapping of Histone Modifications at DNA Double-Strand Breaks Deciphers Repair Pathway Chromatin Signatures. Mol Cell. 2018;72:250-262.e6 pubmed publisher
Sheng W, LaFleur M, Nguyen T, Chen S, Chakravarthy A, Conway J, et al. LSD1 Ablation Stimulates Anti-tumor Immunity and Enables Checkpoint Blockade. Cell. 2018;174:549-563.e19 pubmed publisher
Takeda D, Spisak S, Seo J, Bell C, O CONNOR E, Korthauer K, et al. A Somatically Acquired Enhancer of the Androgen Receptor Is a Noncoding Driver in Advanced Prostate Cancer. Cell. 2018;174:422-432.e13 pubmed publisher
Link V, Duttke S, Chun H, Holtman I, Westin E, Hoeksema M, et al. Analysis of Genetically Diverse Macrophages Reveals Local and Domain-wide Mechanisms that Control Transcription Factor Binding and Function. Cell. 2018;173:1796-1809.e17 pubmed publisher
Zheng Y, Xue M, Shen H, Li X, Ma D, Gong Y, et al. PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression. Cancer Res. 2018;78:3190-3206 pubmed publisher
Kim H, Tan Y, Ma W, Merkurjev D, Destici E, Ma Q, et al. Pluripotency factors functionally premark cell-type-restricted enhancers in ES cells. Nature. 2018;556:510-514 pubmed publisher
Anan K, Hino S, Shimizu N, Sakamoto A, Nagaoka K, Takase R, et al. LSD1 mediates metabolic reprogramming by glucocorticoids during myogenic differentiation. Nucleic Acids Res. 2018;46:5441-5454 pubmed publisher
Sobol M, Krausová A, Yildirim S, Kalasová I, Fáberová V, Vrkoslav V, et al. Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription. J Cell Sci. 2018;131: pubmed publisher
Fueyo R, Iacobucci S, PAPPA S, Estarás C, Lois S, Vicioso Mantis M, et al. Lineage specific transcription factors and epigenetic regulators mediate TGF?-dependent enhancer activation. Nucleic Acids Res. 2018;46:3351-3365 pubmed publisher
Leadem B, Kagiampakis I, Wilson C, Cheung T, Arnott D, Trojer P, et al. A KDM5 Inhibitor Increases Global H3K4 Trimethylation Occupancy and Enhances the Biological Efficacy of 5-Aza-2'-Deoxycytidine. Cancer Res. 2018;78:1127-1139 pubmed publisher
Tang S, Fang Y, Huang G, Xu X, Padilla Banks E, Fan W, et al. Methionine metabolism is essential for SIRT1-regulated mouse embryonic stem cell maintenance and embryonic development. EMBO J. 2017;36:3175-3193 pubmed publisher
Wu D, Zhao L, Feng Z, Yu C, Ding J, Wang L, et al. O-Linked N-acetylglucosamine transferase 1 regulates global histone H4 acetylation via stabilization of the nonspecific lethal protein NSL3. J Biol Chem. 2017;292:10014-10025 pubmed publisher
Byun S, Kim Y, Zhang Y, Kong B, Guo G, Sadoshima J, et al. A postprandial FGF19-SHP-LSD1 regulatory axis mediates epigenetic repression of hepatic autophagy. EMBO J. 2017;36:1755-1769 pubmed publisher
Carnesecchi J, Forcet C, Zhang L, Tribollet V, Barenton B, Boudra R, et al. ERR? induces H3K9 demethylation by LSD1 to promote cell invasion. Proc Natl Acad Sci U S A. 2017;114:3909-3914 pubmed publisher
Brenner C, Luciani J, Bizet M, Ndlovu M, Josseaux E, Dedeurwaerder S, et al. The interplay between the lysine demethylase KDM1A and DNA methyltransferases in cancer cells is cell cycle dependent. Oncotarget. 2016;7:58939-58952 pubmed publisher
Ibiza S, García Cassani B, Ribeiro H, Carvalho T, Almeida L, Marques R, et al. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence. Nature. 2016;535:440-443 pubmed publisher
Blazer L, Lima Fernandes E, Gibson E, Eram M, Loppnau P, Arrowsmith C, et al. PR Domain-containing Protein 7 (PRDM7) Is a Histone 3 Lysine 4 Trimethyltransferase. J Biol Chem. 2016;291:13509-19 pubmed publisher
Beshiri M, Holmes K, Richter W, HESS S, Islam A, Yan Q, et al. Coordinated repression of cell cycle genes by KDM5A and E2F4 during differentiation. Proc Natl Acad Sci U S A. 2012;109:18499-504 pubmed publisher
Ooi S, Priess J, Henikoff S. Histone H3.3 variant dynamics in the germline of Caenorhabditis elegans. PLoS Genet. 2006;2:e97 pubmed
product information
Catalog Number :
07-030
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-dimethyl-Histone H3 (Lys4) Antibody
Product Type :
Antibodies
Clonality :
Polyclonal Antibody
Gene ID :
Q16695
Host Name :
Rabbit
Antigen :
dimethyl-Histone H3 (Lys4)
Conjugate :
Unpurified
Product Description :
Anti-dimethyl-Histone H3 (Lys4) Antibody
Cross Reactivity :
Human;Tetrahymena
Background :
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
ALT Names :
H3K4me2;Histone H3 (di methyl K4);H3 histone family, member T;histone 3, H3;histone cluster 3, H3
Immunogen :
KLH conjugated synthetic peptide (ARTMe2KQTAR-GC) corresponding to amino acids 1-8 of human Histone H3.
Specificity :
Recognizes dimethylated Histone H3 (Lys4), MW ~17 kDa. Specificity demonstrated by ELISA using methylated peptides.
Package Size :
200 µL
Uses :
Dot Blot;Immunocytochemistry;Immunofluorescence;Western Blotting;Peptide Inhibition Assay;ChIP-seq;Chromatin Immunoprecipitation (ChIP)
Storage :
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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