This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
acetyl-Histone H3 (Lys9) Antibody
catalog :
06-942
quantity :
200 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - frozen section, ChIP-Seq, other
citations: 29
Published Application/Species/Sample/DilutionReference
  • ChIP-Seq; human; loading ...; fig 9
Meyer M, Benkusky N, Kaufmann M, Lee S, Redfield R, Jones G, et al. Targeted genomic deletions identify diverse enhancer functions and generate a kidney-specific, endocrine-deficient Cyp27b1 pseudo-null mouse. J Biol Chem. 2019;294:9518-9535 pubmed publisher
  • western blot; human; loading ...; fig s6d
Wei X, Guo J, Li Q, Jia Q, Jing Q, Li Y, et al. Bach1 regulates self-renewal and impedes mesendodermal differentiation of human embryonic stem cells. Sci Adv. 2019;5:eaau7887 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig s10
Chen X, Zhi X, Wang J, Su J. RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation. Bone Res. 2018;6:34 pubmed publisher
  • western blot; mouse; loading ...; fig 5a
Bartova E, Lochmanová G, Legartova S, Suchankova J, Fedr R, Krejci J, et al. Irradiation by γ-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and γH2AX. Biochimie. 2018;154:86-98 pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig 7c
Meyer M, Benkusky N, Kaufmann M, Lee S, Onal M, Jones G, et al. A kidney-specific genetic control module in mice governs endocrine regulation of the cytochrome P450 gene Cyp27b1 essential for vitamin D3 activation. J Biol Chem. 2017;292:17541-17558 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3a
Krejci J, Legartova S, Bartova E. Neural Differentiation in HDAC1-Depleted Cells Is Accompanied by Coilin Downregulation and the Accumulation of Cajal Bodies in Nucleoli. Stem Cells Int. 2017;2017:1021240 pubmed publisher
  • immunocytochemistry; mouse; loading ...; fig S3A
  • western blot; mouse; loading ...; fig 6G
Barateau A, Vadrot N, Vicart P, Ferreiro A, Mayer M, Heron D, et al. A Novel Lamin A Mutant Responsible for Congenital Muscular Dystrophy Causes Distinct Abnormalities of the Cell Nucleus. PLoS ONE. 2017;12:e0169189 pubmed publisher
  • western blot; human; loading ...; fig 1c
Bahr J, Robey R, Luchenko V, Basseville A, Chakraborty A, Kozlowski H, et al. Blocking downstream signaling pathways in the context of HDAC inhibition promotes apoptosis preferentially in cells harboring mutant Ras. Oncotarget. 2016;7:69804-69815 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig s4d
Wang D, Kon N, Lasso G, Jiang L, Leng W, Zhu W, et al. Acetylation-regulated interaction between p53 and SET reveals a widespread regulatory mode. Nature. 2016;538:118-122 pubmed publisher
  • immunocytochemistry; mouse; fig 5
Mansuroglu Z, Benhelli Mokrani H, Marcato V, Sultan A, Violet M, Chauderlier A, et al. Loss of Tau protein affects the structure, transcription and repair of neuronal pericentromeric heterochromatin. Sci Rep. 2016;6:33047 pubmed publisher
  • western blot; human; 1:1000; fig 3
Penterling C, Drexler G, Böhland C, Stamp R, Wilke C, Braselmann H, et al. Depletion of Histone Demethylase Jarid1A Resulting in Histone Hyperacetylation and Radiation Sensitivity Does Not Affect DNA Double-Strand Break Repair. PLoS ONE. 2016;11:e0156599 pubmed publisher
  • western blot; human; loading ...; fig 2a
Shirakawa K, Wang L, Man N, Maksimoska J, Sorum A, Lim H, et al. Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity. elife. 2016;5: pubmed publisher
  • western blot; human; fig 2
Onyango D, Howard S, Neherin K, Yanez D, Stark J. Tetratricopeptide repeat factor XAB2 mediates the end resection step of homologous recombination. Nucleic Acids Res. 2016;44:5702-16 pubmed publisher
  • other; human; fig 5
  • western blot; human; 1:1000; fig 5
Pan H, Guan D, Liu X, Li J, Wang L, Wu J, et al. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Res. 2016;26:190-205 pubmed publisher
  • western blot; mouse; fig 3g
Hagelkruys A, Mattes K, Moos V, Rennmayr M, Ringbauer M, Sawicka A, et al. Essential Nonredundant Function of the Catalytic Activity of Histone Deacetylase 2 in Mouse Development. Mol Cell Biol. 2016;36:462-74 pubmed publisher
Legartova S, Lochmanová G, Zdrahal Z, Kozubek S, Sponer J, Krepl M, et al. DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1β-Ser88. Cells. 2019;8: pubmed publisher
Meyer M, Lee S, Carlson A, Benkusky N, Kaufmann M, Jones G, et al. A chromatin-based mechanism controls differential regulation of the cytochrome P450 gene Cyp24a1 in renal and non-renal tissues. J Biol Chem. 2019;294:14467-14481 pubmed publisher
Rutowicz K, Lirski M, Mermaz B, Teano G, Schubert J, Mestiri I, et al. Linker histones are fine-scale chromatin architects modulating developmental decisions in Arabidopsis. Genome Biol. 2019;20:157 pubmed publisher
Maes M, Colombo G, Schulz R, Siegert S. Targeting microglia with lentivirus and AAV: Recent advances and remaining challenges. Neurosci Lett. 2019;707:134310 pubmed publisher
Tang T, Zhang Y, Wang Y, Cai Z, Lu Z, Li L, et al. HDAC1 and HDAC2 Regulate Intermediate Progenitor Positioning to Safeguard Neocortical Development. Neuron. 2019;101:1117-1133.e5 pubmed publisher
Fenizia C, Bottino C, Corbetta S, Fittipaldi R, Floris P, Gaudenzi G, et al. SMYD3 promotes the epithelial-mesenchymal transition in breast cancer. Nucleic Acids Res. 2019;47:1278-1293 pubmed publisher
Svobodová Kovaříková A, Legartova S, Krejci J, Bartova E. H3K9me3 and H4K20me3 represent the epigenetic landscape for 53BP1 binding to DNA lesions. Aging (Albany NY). 2018;10:2585-2605 pubmed publisher
Zhang W, Wan H, Feng G, Qu J, Wang J, Jing Y, et al. SIRT6 deficiency results in developmental retardation in cynomolgus monkeys. Nature. 2018;560:661-665 pubmed publisher
Arcidiacono O, Krejci J, Suchankova J, Bartova E. Deacetylation of Histone H4 Accompanying Cardiomyogenesis is Weakened in HDAC1-Depleted ES Cells. Int J Mol Sci. 2018;19: pubmed publisher
Read K, Powell M, Baker C, Sreekumar B, Ringel Scaia V, Bachus H, et al. Integrated STAT3 and Ikaros Zinc Finger Transcription Factor Activities Regulate Bcl-6 Expression in CD4+ Th Cells. J Immunol. 2017;199:2377-2387 pubmed publisher
Choi J, Yoon S, Kim S, Ahn Jo S. KDM4B histone demethylase and G9a regulate expression of vascular adhesion proteins in cerebral microvessels. Sci Rep. 2017;7:45005 pubmed publisher
Feldman S, Wuerffel R, Achour I, Wang L, Carpenter P, Kenter A. 53BP1 Contributes to Igh Locus Chromatin Topology during Class Switch Recombination. J Immunol. 2017;198:2434-2444 pubmed publisher
Mandal M, Hamel K, Maienschein Cline M, Tanaka A, Teng G, Tuteja J, et al. Histone reader BRWD1 targets and restricts recombination to the Igk locus. Nat Immunol. 2015;16:1094-103 pubmed publisher
Guenatri M, Bailly D, Maison C, Almouzni G. Mouse centric and pericentric satellite repeats form distinct functional heterochromatin. J Cell Biol. 2004;166:493-505 pubmed
product information
Catalog Number :
06-942
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-acetyl-Histone H3 (Lys9) Antibody
Product Type :
Antibodies
Clonality :
Polyclonal Antibody
Gene ID :
Q16695
Host Name :
Rabbit
Antigen :
acetyl-Histone H3 (Lys9)
Conjugate :
Purified
Isotype :
IgG
Product Description :
Anti-acetyl-Histone H3 (Lys9) Antibody
Cross Reactivity :
Human;Mouse;Rat;Eukaryote
Background :
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
ALT Names :
H3K9Ac;Histone H3 (acetyl K9);H3 histone family, member T;histone 3, H3;histone cluster 3, H3
Immunogen :
KLH-conjugated, synthetic peptide (ARTKQTA RAcKSTG-C) corresponding to amino acids 1-12 of Histone H3.
Specificity :
Histone H3 acetylated at position 9.
Package Size :
200 µg
Uses :
Dot Blot;Western Blotting;ChIP-seq
Storage :
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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