Cdk1/Cdc2 (PSTAIR) Antibody | EMD Millipore 06-923 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Cdk1/Cdc2 (PSTAIR) Antibody

catalog :

06-923

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, chicken

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 2b	Mukherjee S, Chakraborty P, Saha P. Phosphorylation of Ku70 subunit by cell cycle kinases modulates the replication related function of Ku heterodimer. Nucleic Acids Res. 2016;44:7755-65 pubmed publisher
	Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, et al. An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol. 2007;178:257-68 pubmed

product information

Catalog Number :

06-923

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-Cdk1/Cdc2 (PSTAIR) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P06493

Host Name :

Rabbit

Antigen :

Cdk1/Cdc2 (PSTAIR)

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-Cdk1/Cdc2 (PSTAIR) Antibody

Cross Reactivity :

Amphibian;Chicken;Human;Mouse;Rat;Sheep;Drosophila;Monkey;Canine

Background :

Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Expression of cdc2 plays a critical role in cell transition through the G2/M phase. The activity of cdc2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for cdc2.

Immunogen :

Synthetic peptide corresponding to residues 42-57 of human cdc2 kinase. The immunogen is identical in a wide range of species.

Specificity :

Cdk1/cdc2 kinase

Package Size :

100 µg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of receipt