product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
acetyl-Histone H4 Antibody
catalog :
06-866
quantity :
200 µL
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
Entamoeba histolytica, human, mouse, bovine
application :
western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, ChIP-Seq
more info or order :
citations: 52
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 2b
Qiu J, Villa M, Sanin D, Buck M, O Sullivan D, Ching R, et al. Acetate Promotes T Cell Effector Function during Glucose Restriction. Cell Rep. 2019;27:2063-2074.e5 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 1d
Song K, Kim J, Lee Y, Bae H, Lee H, Woo S, et al. Mitochondrial reprogramming via ATP5H loss promotes multimodal cancer therapy resistance. J Clin Invest. 2018;128:4098-4114 pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 4a
Sacta M, Tharmalingam B, Coppo M, Rollins D, Deochand D, Benjamin B, et al. Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages. elife. 2018;7: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1a
Schmitt H, Schlamp C, Nickells R. Targeting HDAC3 Activity with RGFP966 Protects Against Retinal Ganglion Cell Nuclear Atrophy and Apoptosis After Optic Nerve Injury. J Ocul Pharmacol Ther. 2018;34:260-273 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig s5a
  • western blot; human; loading ...; fig s5a
Liang Z, Brown K, Carroll T, Taylor B, Vidal I, Hendrich B, et al. A high-resolution map of transcriptional repression. elife. 2017;6: pubmed publisher
  • chromatin immunoprecipitation; mouse; loading ...; fig 1d
Cheung K, Zhang F, Jaganathan A, Sharma R, Zhang Q, Konuma T, et al. Distinct Roles of Brd2 and Brd4 in Potentiating the Transcriptional Program for Th17 Cell Differentiation. Mol Cell. 2017;65:1068-1080.e5 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 6b
Lin Y, Lin Y, Huang M, Kuo P, Wu C, Lee M, et al. Tumor necrosis factor-alpha inhibitors suppress CCL2 chemokine in monocytes via epigenetic modification. Mol Immunol. 2017;83:82-91 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig s4d
Wang D, Kon N, Lasso G, Jiang L, Leng W, Zhu W, et al. Acetylation-regulated interaction between p53 and SET reveals a widespread regulatory mode. Nature. 2016;538:118-122 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 7d
Gay G, Braun L, Brenier Pinchart M, Vollaire J, Josserand V, Bertini R, et al. Toxoplasma gondii TgIST co-opts host chromatin repressors dampening STAT1-dependent gene regulation and IFN-?-mediated host defenses. J Exp Med. 2016;213:1779-98 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 4c
Hong S, Eun J, Choi S, Shen Q, Choi W, Han J, et al. Epigenetic reader BRD4 inhibition as a therapeutic strategy to suppress E2F2-cell cycle regulation circuit in liver cancer. Oncotarget. 2016;7:32628-40 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 4
Ma X, Xu L, Mueller E. Forkhead box A3 mediates glucocorticoid receptor function in adipose tissue. Proc Natl Acad Sci U S A. 2016;113:3377-82 pubmed publisher
  • western blot; mouse; 1:2000; fig s13
Karuppagounder S, Alim I, Khim S, Bourassa M, Sleiman S, John R, et al. Therapeutic targeting of oxygen-sensing prolyl hydroxylases abrogates ATF4-dependent neuronal death and improves outcomes after brain hemorrhage in several rodent models. Sci Transl Med. 2016;8:328ra29 pubmed publisher
  • ChIP-Seq; mouse; fig 6
  • western blot; mouse; fig 3b
Gonzales Cope M, Sidoli S, Bhanu N, Won K, Garcia B. Histone H4 acetylation and the epigenetic reader Brd4 are critical regulators of pluripotency in embryonic stem cells. BMC Genomics. 2016;17:95 pubmed publisher
  • western blot; bovine; 1:4000; fig 2
  • immunocytochemistry; Entamoeba histolytica; 1:1500; fig 3
  • western blot; Entamoeba histolytica; 1:4000; fig 2
Lozano Amado D, Herrera Solorio A, Valdés J, Alemán Lazarini L, Almaraz Barrera M, Luna Rivera E, et al. Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica. Parasit Vectors. 2016;9:19 pubmed publisher
  • western blot; human; fig 3
Seidel C, Schnekenburger M, Mazumder A, Teiten M, Kirsch G, Dicato M, et al. 4-Hydroxybenzoic acid derivatives as HDAC6-specific inhibitors modulating microtubular structure and HSP90α chaperone activity against prostate cancer. Biochem Pharmacol. 2016;99:31-52 pubmed publisher
Wu R, Young I, Chen Y, Chuang S, Toubaji A, Wu M. Identification of the PTEN-ARID4B-PI3K pathway reveals the dependency on ARID4B by PTEN-deficient prostate cancer. Nat Commun. 2019;10:4332 pubmed publisher
Horvath A, Dániel B, Szeles L, Cuaranta Monroy I, Czimmerer Z, Ozgyin L, et al. Labelled regulatory elements are pervasive features of the macrophage genome and are dynamically utilized by classical and alternative polarization signals. Nucleic Acids Res. 2019;47:2778-2792 pubmed publisher
Engel M, Gee Y, Cross D, Maccarone A, Heng B, Hulme A, et al. Novel dual-action prodrug triggers apoptosis in glioblastoma cells by releasing a glutathione quencher and lysine-specific histone demethylase 1A inhibitor. J Neurochem. 2019;149:535-550 pubmed publisher
Kumar M, Kasti K, Balakrishnan L, Milavetz B. Directed Nucleosome Sliding during the Formation of the Simian Virus 40 Particle Exposes DNA Sequences Required for Early Transcription. J Virol. 2019;93: pubmed publisher
Belloni L, Di Cocco S, Guerrieri F, Nunn A, Piconese S, Salerno D, et al. Targeting a phospho-STAT3-miRNAs pathway improves vesicular hepatic steatosis in an in vitro and in vivo model. Sci Rep. 2018;8:13638 pubmed publisher
Kamada R, Yang W, Zhang Y, Patel M, Yang Y, Ouda R, et al. Interferon stimulation creates chromatin marks and establishes transcriptional memory. Proc Natl Acad Sci U S A. 2018;115:E9162-E9171 pubmed publisher
de Bruyn Kops A, Burke J, Guthrie C. Brr6 plays a role in gene recruitment and transcriptional regulation at the nuclear envelope. Mol Biol Cell. 2018;29:2578-2590 pubmed publisher
Bruzzone M, Grünberg S, Kubik S, Zentner G, Shore D. Distinct patterns of histone acetyltransferase and Mediator deployment at yeast protein-coding genes. Genes Dev. 2018;32:1252-1265 pubmed publisher
Zhao Z, Sentürk N, Song C, Grummt I. lncRNA PAPAS tethered to the rDNA enhancer recruits hypophosphorylated CHD4/NuRD to repress rRNA synthesis at elevated temperatures. Genes Dev. 2018;32:836-848 pubmed publisher
Ruiz L, Gurlo T, Ravier M, Wojtusciszyn A, Mathieu J, Brown M, et al. Proteasomal degradation of the histone acetyl transferase p300 contributes to beta-cell injury in a diabetes environment. Cell Death Dis. 2018;9:600 pubmed publisher
Cai S, Song Y, Chen C, Shi J, Gan L. Natural chromatin is heterogeneous and self-associates in vitro. Mol Biol Cell. 2018;29:1652-1663 pubmed publisher
Zhang Y, Wang S, Kang W, Liu C, Dong Y, Ren F, et al. CREPT facilitates colorectal cancer growth through inducing Wnt/β-catenin pathway by enhancing p300-mediated β-catenin acetylation. Oncogene. 2018;37:3485-3500 pubmed publisher
Campbell A, Shadle S, Jagannathan S, Lim J, Resnick R, Tawil R, et al. NuRD and CAF-1-mediated silencing of the D4Z4 array is modulated by DUX4-induced MBD3L proteins. elife. 2018;7: pubmed publisher
Nishitsuji H, Ujino S, Harada K, Shimotohno K. TIP60 Complex Inhibits Hepatitis B Virus Transcription. J Virol. 2018;92: pubmed publisher
Griggs C, Malm S, Jaime Frias R, Smith C. Valproic acid disrupts the oscillatory expression of core circadian rhythm transcription factors. Toxicol Appl Pharmacol. 2018;339:110-120 pubmed publisher
Villain G, Poissonnier L, Noueihed B, Bonfils G, Rivera J, Chemtob S, et al. miR-126-5p promotes retinal endothelial cell survival through SetD5 regulation in neurons. Development. 2018;145: pubmed publisher
Kiese K, Jablonski J, Hackenbracht J, Wrosch J, Groemer T, Kornhuber J, et al. Epigenetic control of epilepsy target genes contributes to a cellular memory of epileptogenesis in cultured rat hippocampal neurons. Acta Neuropathol Commun. 2017;5:79 pubmed publisher
Saavedra F, Rivera C, Rivas E, Merino P, Garrido D, Hernandez S, et al. PP32 and SET/TAF-I? proteins regulate the acetylation of newly synthesized histone H4. Nucleic Acids Res. 2017;45:11700-11710 pubmed publisher
Sepp M, Vihma H, Nurm K, Urb M, Page S, Roots K, et al. The Intellectual Disability and Schizophrenia Associated Transcription Factor TCF4 Is Regulated by Neuronal Activity and Protein Kinase A. J Neurosci. 2017;37:10516-10527 pubmed publisher
Boudoures A, Pfeil J, Steenkiste E, Hoffman R, Bailey E, Wilkes S, et al. A Novel Histone Crosstalk Pathway Important for Regulation of UV-Induced DNA Damage Repair in Saccharomyces cerevisiae. Genetics. 2017;206:1389-1402 pubmed publisher
Shimamoto Y, Tamura S, Masumoto H, Maeshima K. Nucleosome-nucleosome interactions via histone tails and linker DNA regulate nuclear rigidity. Mol Biol Cell. 2017;28:1580-1589 pubmed publisher
Guerrieri F, Belloni L, D Andrea D, Pediconi N, Le Pera L, Testoni B, et al. Genome-wide identification of direct HBx genomic targets. BMC Genomics. 2017;18:184 pubmed publisher
Feldman S, Wuerffel R, Achour I, Wang L, Carpenter P, Kenter A. 53BP1 Contributes to Igh Locus Chromatin Topology during Class Switch Recombination. J Immunol. 2017;198:2434-2444 pubmed publisher
Patanun O, Ueda M, Itouga M, Kato Y, Utsumi Y, Matsui A, et al. The Histone Deacetylase Inhibitor Suberoylanilide Hydroxamic Acid Alleviates Salinity Stress in Cassava. Front Plant Sci. 2016;7:2039 pubmed publisher
Rimando M, Wu H, Liu Y, Lee C, Kuo S, Lo Y, et al. Glucocorticoid receptor and Histone deacetylase 6 mediate the differential effect of dexamethasone during osteogenesis of mesenchymal stromal cells (MSCs). Sci Rep. 2016;6:37371 pubmed publisher
Xu X, Xu S, Fu L, Hu Q, Lv Y, Du L. Epigenetic changes in peripheral leucocytes as biomarkers in intrauterine growth retardation rat. Biomed Rep. 2016;5:548-552 pubmed
Contreras Jurado C, Alonso Merino E, Saiz Ladera C, Valiño A, Regadera J, Alemany S, et al. The Thyroid Hormone Receptors Inhibit Hepatic Interleukin-6 Signaling During Endotoxemia. Sci Rep. 2016;6:30990 pubmed publisher
Cheng T, Thompson D, O Mara T, Painter J, Glubb D, Flach S, et al. Five endometrial cancer risk loci identified through genome-wide association analysis. Nat Genet. 2016;48:667-674 pubmed publisher
Shi G, Yoshida Y, Yuki K, Nishimura T, Kawata Y, Kawashima M, et al. Pattern of RECK CpG methylation as a potential marker for predicting breast cancer prognosis and drug-sensitivity. Oncotarget. 2016;7:82158-82169 pubmed publisher
Chen J, Zheng Z, Chen Y, Li J, Qian S, Shi Y, et al. Histone Deacetylase Inhibitors Trichostatin A and MCP30 Relieve Benzene-Induced Hematotoxicity via Restoring Topoisomerase II?. PLoS ONE. 2016;11:e0153330 pubmed publisher
Rios E, Soriano F, Olah G, Gero D, Szczesny B, Szabo C. Hydrogen sulfide modulates chromatin remodeling and inflammatory mediator production in response to endotoxin, but does not play a role in the development of endotoxin tolerance. J Inflamm (Lond). 2016;13:10 pubmed publisher
Gazdag E, Jacobi U, van Kruijsbergen I, Weeks D, Veenstra G. Activation of a T-box-Otx2-Gsc gene network independent of TBP and TBP-related factors. Development. 2016;143:1340-50 pubmed publisher
Akiyama Y, Koda Y, Byeon S, Shimada S, Nishikawaji T, Sakamoto A, et al. Reduced expression of SET7/9, a histone mono-methyltransferase, is associated with gastric cancer progression. Oncotarget. 2016;7:3966-83 pubmed publisher
Morozumi Y, Boussouar F, Tan M, Chaikuad A, Jamshidikia M, Colak G, et al. Atad2 is a generalist facilitator of chromatin dynamics in embryonic stem cells. J Mol Cell Biol. 2016;8:349-62 pubmed publisher
Palmisano I, Della Chiara G, D Ambrosio R, Huichalaf C, Brambilla P, Corbetta S, et al. Amino acid starvation induces reactivation of silenced transgenes and latent HIV-1 provirus via down-regulation of histone deacetylase 4 (HDAC4). Proc Natl Acad Sci U S A. 2012;109:E2284-93 pubmed publisher
Mandal M, Powers S, Maienschein Cline M, Bartom E, Hamel K, Kee B, et al. Epigenetic repression of the Igk locus by STAT5-mediated recruitment of the histone methyltransferase Ezh2. Nat Immunol. 2011;12:1212-20 pubmed publisher
Lalmansingh A, Uht R. Estradiol regulates corticotropin-releasing hormone gene (crh) expression in a rapid and phasic manner that parallels estrogen receptor-alpha and -beta recruitment to a 3',5'-cyclic adenosine 5'-monophosphate regulatory region of the proximal crh pro. Endocrinology. 2008;149:346-57 pubmed
product information
Catalog Number :
06-866
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-acetyl-Histone H4 Antibody
Product Type :
Antibodies
Clonality :
Polyclonal Antibody
Gene ID :
P62805
Host Name :
Rabbit
Antigen :
acetyl-Histone H4
Conjugate :
Serum
Isotype :
IgG
Product Description :
Anti-acetyl-Histone H4 Antibody
Cross Reactivity :
Human;Tetrahymena;Eukaryote
Background :
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
ALT Names :
H4 histone family, member A;histone 1, H4a;histone cluster 1, H4a
Immunogen :
KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16.
Specificity :
Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins.
Package Size :
200 µL
Uses :
Western Blotting;Immunocytochemistry;Chromatin Immunoprecipitation (ChIP)
Storage :
Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
more info or order :
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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