acetyl-p53 Antibody (Lys373, Lys382) | EMD Millipore 06-758 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

acetyl-p53 Antibody (Lys373, Lys382)

catalog :

06-758

quantity :

200 μL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; fig EV1C-E	Hui X, Zhang M, Gu P, Li K, Gao Y, Wu D, et al. Adipocyte SIRT1 controls systemic insulin sensitivity by modulating macrophages in adipose tissue. EMBO Rep. 2017;18:645-657 pubmed publisher

product information

Catalog Number :

06-758

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-acetyl-p53 Antibody (Lys373, Lys382)

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P04637

Host Name :

Rabbit

Antigen :

acetyl-p53 (Lys373

Conjugate :

Serum

Isotype :

IgG

Product Description :

Anti-acetyl-p53 Antibody (Lys373, Lys382)

Cross Reactivity :

Vertebrates

Background :

p53 is a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers.

Immunogen :

peptide of human p53 corresponding to amino acids 368-389 with a duplication of amino acids 376 and 377 (HLKSKACKGQSTSTSRHKACKLMFKTC) where ACK denotes acetyl-lysine

Specificity :

Recognizes p53 acetylated in vitro by recombinant p300, and for peptide containing acetyl-lysine 373 but not for the peptide containing acetyl-lysine 320.

Package Size :

200 μL

Uses :

Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.