phospho-Smad1 (Ser463/465) Antibody | EMD Millipore 06-702 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Smad1 (Ser463/465) Antibody

catalog :

06-702

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot

product information

Catalog Number :

06-702

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Smad1 (Ser463/465) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q15797

Host Name :

Rabbit

Antigen :

phospho-Smad1 (Ser463/465)

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-phospho-Smad1 (Ser463/465) Antibody

Cross Reactivity :

Human;Mouse;Rat;Xenopus

Background :

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), function downstream of TGF-β receptor Serine/Threonine kinases and undergo serine phosphorylation in response to receptor activation. Following BMP (bone morphogenic protein) or TGF-β binding to the targeted surface receptors, Smad1 (also designated Madr1 or JV4-1) becomes phosphorylated at Ser 463 and Ser 465. Activated Smad1 associates with Smad4 and translocates to the nucleus, where the Smad1/Smad4 complex recruits DNA-binding proteins to activate specific gene transcription. BMP binding induces phosphorylation of Smad1, which enhances the binding of Smad1 to CBP to stimulate Smad1-dependent transcription. In addition, ALK1 and ALK2 specifically phosphorylate Smad1 on residues located in the MH2 domain and induces Smad1-dependent pathways.

Immunogen :

peptide corresponding to (CGGSPHNPISpSVpSGG) corresponding to amino acids 455-465 of human Smad1 protein

Specificity :

Recognizes the dual serine phosphorylated Smad1 (Ser463/465).

Package Size :

100 μg

Uses :

Western Blotting

Storage :

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.