TrkA Antibody | EMD Millipore 06-574 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

TrkA Antibody

catalog :

06-574

quantity :

200 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 1c	Rizzi C, Tiberi A, Giustizieri M, Marrone M, Gobbo F, Carucci N, et al. NGF steers microglia toward a neuroprotective phenotype. Glia. 2018;66:1395-1416 pubmed publisher
western blot; mouse; fig 4a	Pediaditakis I, Kourgiantaki A, Prousis K, Potamitis C, Xanthopoulos K, Zervou M, et al. BNN27, a 17-Spiroepoxy Steroid Derivative, Interacts With and Activates p75 Neurotrophin Receptor, Rescuing Cerebellar Granule Neurons from Apoptosis. Front Pharmacol. 2016;7:512 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:250; fig 2	Bouilloux F, Thireau J, VentÃ©o S, Farah C, Karam S, Dauvilliers Y, et al. Loss of the transcription factor Meis1 prevents sympathetic neurons target-field innervation and increases susceptibility to sudden cardiac death. elife. 2016;5: pubmed publisher
	Paixão S, Loschek L, Gaitanos L, Alcalà Morales P, Goulding M, Klein R. Identification of Spinal Neurons Contributing to the Dorsal Column Projection Mediating Fine Touch and Corrective Motor Movements. Neuron. 2019;104:749-764.e6 pubmed publisher
	Crerar H, Scott Solomon E, Bodkin Clarke C, Andreassi C, Hazbon M, Logie E, et al. Regulation of NGF Signaling by an Axonal Untranslated mRNA. Neuron. 2019;102:553-563.e8 pubmed publisher
	Carlin D, Golden J, Mogha A, Samineni V, Monk K, Gereau R, et al. Deletion of Tsc2 in Nociceptors Reduces Target Innervation, Ion Channel Expression, and Sensitivity to Heat. Eneuro. 2018;5: pubmed publisher
	Xu W, Weissmiller A, White J, Fang F, Wang X, Wu Y, et al. Amyloid precursor protein-mediated endocytic pathway disruption induces axonal dysfunction and neurodegeneration. J Clin Invest. 2016;126:1815-33 pubmed publisher

product information

Catalog Number :

06-574

Subcategory :

Neuroscience

Product Name :

Anti-TrkA Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P04629

Host Name :

Rabbit

Antigen :

TrkA

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-TrkA Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

TrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.

ALT Names :

Neurotrophic tyrosine kinase receptor type;Oncogene TRK;TRK1-transforming tyrosine kinase protein;high affinity nerve growth factor receptor;neurotrophic tyrosine kinase, receptor, type 1;tyrosine kinase receptor A

Immunogen :

Rabbits immunized with bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.

Specificity :

Recognizes TrkA, Mr 140 kDa. Does not cross-react with TrkB or TrkC.

Package Size :

200 µg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.