iNOS/NOS II Antibody, NT | EMD Millipore 06-573 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

iNOS/NOS II Antibody, NT

catalog :

06-573

quantity :

200 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse, bovine

application :

western blot, immunohistochemistry, immunoprecipitation, immunohistochemistry - frozen section

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:250; loading ...; fig 3f	Yagura K, Ohtaki H, Tsumuraya T, Sato A, Miyamoto K, Kawada N, et al. The enhancement of CCL2 and CCL5 by human bone marrow-derived mesenchymal stem/stromal cells might contribute to inflammatory suppression and axonal extension after spinal cord injury. PLoS ONE. 2020;15:e0230080 pubmed publisher
western blot; mouse; 1:5000; loading ...; fig 1a	Nakazawa H, Chang K, Shinozaki S, Yasukawa T, Ishimaru K, Yasuhara S, et al. iNOS as a Driver of Inflammation and Apoptosis in Mouse Skeletal Muscle after Burn Injury: Possible Involvement of Sirt1 S-Nitrosylation-Mediated Acetylation of p65 NF-κB and p53. PLoS ONE. 2017;12:e0170391 pubmed publisher
	Shen Y, Kapfhamer D, Minnella A, Kim J, Won S, Chen Y, et al. Bioenergetic state regulates innate inflammatory responses through the transcriptional co-repressor CtBP. Nat Commun. 2017;8:624 pubmed publisher

product information

Catalog Number :

06-573

Subcategory :

Neuroscience

Product Name :

Anti-iNOS/NOS II Antibody, NT

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P35228

Host Name :

Rabbit

Antigen :

iNOS/NOS II

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-iNOS/NOS II Antibody, NT

Cross Reactivity :

Mouse;Bovine;Sheep;Guinea Pig

Background :

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

ALT Names :

Hepatocyte NOS 3;Inducible NO synthase 3;Inducible NOS 3;NOS type II 3;NOS, type II 2;nitric oxide synthase 2, inducible;nitric oxide synthase 2A;nitric oxide synthase 2A (inducible, hepatocytes);nitric oxide synthase, macrophage 2

Immunogen :

Partial fusion protein raised against the N-terminus of murine iNOS/NOS II.

Specificity :

Recognizes N-terminus of iNOS, Mr 125 kDa. Does not cross-react with nNOS/NOS I.

Package Size :

200 µg

Uses :

Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.