phospho-Histone H3 (Ser10) Antibody, Mitosis Marker | EMD Millipore 06-570 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Histone H3 (Ser10) Antibody, Mitosis Marker

catalog :

06-570

quantity :

200 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

Japanese rice fish, common cuttlefish, axolotl, Holothuria glaberrima, Austrofundulus limnaeus, human, mouse, rat, chicken, zebrafish , fruit fly , Xenopus laevis

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section

citations: 239

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - free floating section; mouse; 1:500; loading ...; fig 4a	Mancia Leon W, Spatazza J, Rakela B, Chatterjee A, Pande V, Maniatis T, et al. Clustered gamma-protocadherins regulate cortical interneuron programmed cell death. elife. 2020;9: pubmed publisher
western blot; human; loading ...; fig 2a	Douglas P, Ye R, Radhamani S, Cobban A, Jenkins N, Bartlett E, et al. Nocodazole-Induced Expression and Phosphorylation of Anillin and Other Mitotic Proteins Are Decreased in DNA-Dependent Protein Kinase Catalytic Subunit-Deficient Cells and Rescued by Inhibition of the Anaphase-Promoting Complex/Cyclosome with proTAME. Mol Cell Biol. 2020;40: pubmed publisher
western blot; human; 1:1000; loading ...; fig s3e	Viol L, Hata S, Pastor Peidro A, Neuner A, Murke F, Wuchter P, et al. Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis. J Cell Biol. 2020;219: pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig s3f	Mukhtar T, Breda J, Grison A, Karimaddini Z, Grobecker P, Iber D, et al. Tead transcription factors differentially regulate cortical development. Sci Rep. 2020;10:4625 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 4b	Blackwood C, Bailetti A, Nandi S, Gridley T, Hebert J. Notch Dosage: Jagged1 Haploinsufficiency Is Associated With Reduced Neuronal Division and Disruption of Periglomerular Interneurons in Mice. Front Cell Dev Biol. 2020;8:113 pubmed publisher
immunohistochemistry - frozen section; axolotl; loading ...; fig 3h	Sousounis K, Bryant D, Martínez Fernández J, Eddy S, Tsai S, Gundberg G, et al. Eya2 promotes cell cycle progression by regulating DNA damage response during vertebrate limb regeneration. elife. 2020;9: pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000; loading ...; fig s3a	Nomura Komoike K, Saitoh F, Fujieda H. Phosphatidylserine recognition and Rac1 activation are required for Müller glia proliferation, gliosis and phagocytosis after retinal injury. Sci Rep. 2020;10:1488 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 1n	Wang L, Xie J, Zhang H, Tsang L, Tsang S, Braune E, et al. Notch signalling regulates epibranchial placode patterning and segregation. Development. 2020;147: pubmed publisher
immunohistochemistry; human; 1:500; loading ...; fig 6e	Kielkowski P, Buchsbaum I, Kirsch V, Bach N, Drukker M, Cappello S, et al. FICD activity and AMPylation remodelling modulate human neurogenesis. Nat Commun. 2020;11:517 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig s4	Liu H, Mei F, Yang W, Wang H, Wong E, Cai J, et al. Epac1 inhibition ameliorates pathological angiogenesis through coordinated activation of Notch and suppression of VEGF signaling. Sci Adv. 2020;6:eaay3566 pubmed publisher
western blot; human; 1:1000; loading ...; fig 2d	Matsumoto S, Yamamichi T, Shinzawa K, Kasahara Y, Nojima S, Kodama T, et al. GREB1 induced by Wnt signaling promotes development of hepatoblastoma by suppressing TGFβ signaling. Nat Commun. 2019;10:3882 pubmed publisher
immunocytochemistry; human; loading ...; fig s4f	Littler S, Sloss O, Geary B, Pierce A, Whetton A, Taylor S. Oncogenic MYC amplifies mitotic perturbations. Open Biol. 2019;9:190136 pubmed publisher
immunohistochemistry; common cuttlefish; fig 1g	Tarazona O, Lopez D, Slota L, Cohn M. Evolution of limb development in cephalopod mollusks. elife. 2019;8: pubmed publisher
immunohistochemistry; mouse; loading ...; fig 6a	Cuitino M, Pécot T, Sun D, Kladney R, Okano Uchida T, Shinde N, et al. Two Distinct E2F Transcriptional Modules Drive Cell Cycles and Differentiation. Cell Rep. 2019;: pubmed publisher
immunocytochemistry; mouse; 1:500; loading ...; fig 4e	Walton C, Zhang W, Patiño Parrado I, Barrio Alonso E, Garrido J, Frade J. Primary neurons can enter M-phase. Sci Rep. 2019;9:4594 pubmed publisher
western blot; human; 1:1000; loading ...; fig 4e	Frank T, Tuppi M, Hugle M, Dötsch V, van Wijk S, Fulda S. Cell cycle arrest in mitosis promotes interferon-induced necroptosis. Cell Death Differ. 2019;: pubmed publisher
immunohistochemistry; Xenopus laevis; 1:1000; loading ...; fig 5a	Suzuki N, Hirano K, Ogino H, Ochi H. Arid3a regulates nephric tubule regeneration via evolutionarily conserved regeneration signal-response enhancers. elife. 2019;8: pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 6k	Liu S, Hausmann S, CARLSON S, Fuentes M, Francis J, Pillai R, et al. METTL13 Methylation of eEF1A Increases Translational Output to Promote Tumorigenesis. Cell. 2019;176:491-504.e21 pubmed publisher
western blot; mouse; 1:2000; loading ...; fig 1b western blot; human; 1:2000; loading ...; fig 1a	Liccardi G, Ramos Garcia L, Tenev T, Annibaldi A, Legrand A, Robertson D, et al. RIPK1 and Caspase-8 Ensure Chromosome Stability Independently of Their Role in Cell Death and Inflammation. Mol Cell. 2019;73:413-428.e7 pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 1i	Urner S, Planas Paz L, Hilger L, Henning C, Branopolski A, Kelly Goss M, et al. Identification of ILK as a critical regulator of VEGFR3 signalling and lymphatic vascular growth. EMBO J. 2019;38: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 2g	Han S, Dennis D, Balakrishnan A, Dixit R, Britz O, Zinyk D, et al. A non-canonical role for the proneural gene Neurog1 as a negative regulator of neocortical neurogenesis. Development. 2018;145: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 3c	Segarra M, Aburto M, Cop F, Llaó Cid C, Härtl R, Damm M, et al. Endothelial Dab1 signaling orchestrates neuro-glia-vessel communication in the central nervous system. Science. 2018;361: pubmed publisher
immunocytochemistry; mouse; loading ...; fig 5a	Mastromina I, Verrier L, Silva J, Storey K, Dale J. Myc activity is required for maintenance of the neuromesodermal progenitor signalling network and for segmentation clock gene oscillations in mouse. Development. 2018;145: pubmed publisher
western blot; mouse; loading ...; fig 4a	Wang B, Joo J, Mount R, Teubner B, Krenzer A, Ward A, et al. The COPII cargo adapter SEC24C is essential for neuronal homeostasis. J Clin Invest. 2018;128:3319-3332 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 3d	Alonso Martin S, Aurade F, Mademtzoglou D, Rochat A, Zammit P, Relaix F. SOXF factors regulate murine satellite cell self-renewal and function through inhibition of β-catenin activity. elife. 2018;7: pubmed publisher
immunocytochemistry; human; 1:400; loading ...; fig 4b	Ruppert J, Samejima K, Platani M, Molina O, Kimura H, Jeyaprakash A, et al. HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry. EMBO J. 2018;37: pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig s8	Gstrein T, Edwards A, Přistoupilová A, Leca I, Breuss M, Pilat Carotta S, et al. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nat Neurosci. 2018;21:207-217 pubmed publisher
immunohistochemistry; mouse; 1:300; loading ...; fig 4b	Fang J, Coon B, Gillis N, Chen Z, Qiu J, Chittenden T, et al. Shear-induced Notch-Cx37-p27 axis arrests endothelial cell cycle to enable arterial specification. Nat Commun. 2017;8:2149 pubmed publisher
immunohistochemistry; mouse; 1:100; loading ...; fig s3d	Fujita J, Freire P, Coarfa C, Benham A, Gunaratne P, Schneider M, et al. Ronin Governs Early Heart Development by Controlling Core Gene Expression Programs. Cell Rep. 2017;21:1562-1573 pubmed publisher
immunohistochemistry; mouse; loading ...; fig s1f	Vassilev V, Platek A, Hiver S, Enomoto H, Takeichi M. Catenins Steer Cell Migration via Stabilization of Front-Rear Polarity. Dev Cell. 2017;43:463-479.e5 pubmed publisher
immunohistochemistry; zebrafish ; 1:500; loading ...; fig 3e	Escamilla C, Filonova I, Walker A, Xuan Z, Holehonnur R, Espinosa F, et al. Kctd13 deletion reduces synaptic transmission via increased RhoA. Nature. 2017;551:227-231 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig 5b	Tseng K, Danilova T, Domanskyi A, Saarma M, Lindahl M, Airavaara M. MANF Is Essential for Neurite Extension and Neuronal Migration in the Developing Cortex. Eneuro. 2017;4: pubmed publisher
immunohistochemistry - paraffin section; mouse; loading ...; fig 5c	Stopp S, Gründl M, Fackler M, Malkmus J, Leone M, Naumann R, et al. Deletion of Gas2l3 in mice leads to specific defects in cardiomyocyte cytokinesis during development. Proc Natl Acad Sci U S A. 2017;114:8029-8034 pubmed publisher
western blot; human; 1:1000; loading ...; fig s5a	Bleuyard J, Fournier M, Nakato R, Couturier A, Katou Y, Ralf C, et al. MRG15-mediated tethering of PALB2 to unperturbed chromatin protects active genes from genotoxic stress. Proc Natl Acad Sci U S A. 2017;114:7671-7676 pubmed publisher
immunohistochemistry - frozen section; mouse; fig s2i western blot; mouse; loading ...; fig s3o	Ho L, van Dijk M, Chye S, Messerschmidt D, Chng S, Ong S, et al. ELABELA deficiency promotes preeclampsia and cardiovascular malformations in mice. Science. 2017;357:707-713 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:500; fig s4b	Lim J, Ibaseta A, Fischer M, Cancilla B, O Young G, Cristea S, et al. Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer. Nature. 2017;545:360-364 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 5a	Laporte M, Chatellard C, Vauchez V, Hemming F, Deloulme J, Vossier F, et al. Alix is required during development for normal growth of the mouse brain. Sci Rep. 2017;7:44767 pubmed publisher
immunohistochemistry; zebrafish ; 1:3000; loading ...; fig 5a	Liu Y, Sepich D, Solnica Krezel L. Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation. PLoS Genet. 2017;13:e1006564 pubmed publisher
flow cytometry; human; loading ...	Xu H, Di Antonio M, McKinney S, Mathew V, Ho B, O Neil N, et al. CX-5461 is a DNA G-quadruplex stabilizer with selective lethality in BRCA1/2 deficient tumours. Nat Commun. 2017;8:14432 pubmed publisher
immunohistochemistry - frozen section; human; loading ...; fig 3b	Sartore R, Cardoso S, Lages Y, Paraguassu J, Stelling M, Madeiro da Costa R, et al. Trace elements during primordial plexiform network formation in human cerebral organoids. Peerj. 2017;5:e2927 pubmed publisher
immunocytochemistry; mouse; 1:500; loading ...; fig s2a	Gautam J, Nirwane A, Yao Y. Laminin differentially regulates the stemness of type I and type II pericytes. Stem Cell Res Ther. 2017;8:28 pubmed publisher
immunohistochemistry; mouse; 1:300; loading ...; fig 2	Wu N, Jia D, Bates B, Basom R, Eberhart C, MacPherson D. A mouse model of MYCN-driven retinoblastoma reveals MYCN-independent tumor reemergence. J Clin Invest. 2017;127:888-898 pubmed publisher
immunocytochemistry; Japanese rice fish; 1:500; loading ...; fig s10c	Inoue D, Stemmer M, Thumberger T, Ruppert T, Bärenz F, Wittbrodt J, et al. Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses. Nat Commun. 2017;8:14090 pubmed publisher
western blot; human; loading ...; fig 5a	Liu X, Gao Y, Ye H, Gerrin S, Ma F, Wu Y, et al. Positive feedback loop mediated by protein phosphatase 1α mobilization of P-TEFb and basal CDK1 drives androgen receptor in prostate cancer. Nucleic Acids Res. 2017;45:3738-3751 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 2c	Malek Mohammadi M, Kattih B, Grund A, Froese N, Korf Klingebiel M, Gigina A, et al. The transcription factor GATA4 promotes myocardial regeneration in neonatal mice. EMBO Mol Med. 2017;9:265-279 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig 6i-l	Harris L, Zalucki O, Gobius I, McDonald H, Osinki J, Harvey T, et al. Transcriptional regulation of intermediate progenitor cell generation during hippocampal development. Development. 2016;143:4620-4630 pubmed
immunocytochemistry; mouse; 1:100; loading ...	Moyle L, Blanc E, Jaka O, Prueller J, Banerji C, Tedesco F, et al. Ret function in muscle stem cells points to tyrosine kinase inhibitor therapy for facioscapulohumeral muscular dystrophy. elife. 2016;5: pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; tbl 1	Bassett E, Tokarew N, Allemano E, Mazerolle C, Morin K, Mears A, et al. Norrin/Frizzled4 signalling in the preneoplastic niche blocks medulloblastoma initiation. elife. 2016;5: pubmed publisher
western blot; human; loading ...; fig 6a	Guillén Boixet J, Buzon V, Salvatella X, Mendez R. CPEB4 is regulated during cell cycle by ERK2/Cdk1-mediated phosphorylation and its assembly into liquid-like droplets. elife. 2016;5: pubmed publisher
immunohistochemistry; zebrafish ; 1:350; fig s7d	Kantarci H, Gerberding A, Riley B. Spemann organizer gene Goosecoid promotes delamination of neuroblasts from the otic vesicle. Proc Natl Acad Sci U S A. 2016;113:E6840-E6848 pubmed
immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1d	Konstantinidou C, Taraviras S, Pachnis V. Geminin prevents DNA damage in vagal neural crest cells to ensure normal enteric neurogenesis. BMC Biol. 2016;14:94 pubmed
immunocytochemistry; mouse; 1:100; loading ...; fig 4b	Knopp P, Krom Y, Banerji C, Panamarova M, Moyle L, den Hamer B, et al. DUX4 induces a transcriptome more characteristic of a less-differentiated cell state and inhibits myogenesis. J Cell Sci. 2016;129:3816-3831 pubmed
immunocytochemistry; mouse; loading ...; fig s9a western blot; mouse; 1:10,000; loading ...; fig s5j	Kanakkanthara A, Jeganathan K, Limzerwala J, Baker D, Hamada M, Nam H, et al. Cyclin A2 is an RNA binding protein that controls Mre11 mRNA translation. Science. 2016;353:1549-1552 pubmed
immunohistochemistry; mouse; 1:500; loading ...; fig s9	Broix L, Jagline H, Ivanova E, Schmucker S, Drouot N, Clayton Smith J, et al. Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia. Nat Genet. 2016;48:1349-1358 pubmed publisher
immunocytochemistry; human; loading ...; fig 2c	Pozner A, Terooatea T, Buck Koehntop B. Cell-specific Kaiso (ZBTB33) Regulation of Cell Cycle through Cyclin D1 and Cyclin E1. J Biol Chem. 2016;291:24538-24550 pubmed
immunoprecipitation; mouse immunohistochemistry; mouse; fig s5 western blot; mouse; fig 2	Diril M, Bisteau X, Kitagawa M, Caldez M, Wee S, Gunaratne J, et al. Loss of the Greatwall Kinase Weakens the Spindle Assembly Checkpoint. PLoS Genet. 2016;12:e1006310 pubmed publisher
western blot; human; 1:10,000; fig 1	Bertolin G, Sizaire F, Herbomel G, Reboutier D, Prigent C, Tramier M. A FRET biosensor reveals spatiotemporal activation and functions of aurora kinase A in living cells. Nat Commun. 2016;7:12674 pubmed publisher
immunohistochemistry; mouse; 1:1000; loading ...; fig s3b	Xie X, Tsai S, Tsai M. COUP-TFII regulates satellite cell function and muscular dystrophy. J Clin Invest. 2016;126:3929-3941 pubmed publisher
western blot; human; 1:1000; loading ...; fig 4b	Garvanska D, Larsen M, Nilsson J. Synergistic inhibition of the APC/C by the removal of APC15 in HCT116 cells lacking UBE2C. Biol Open. 2016;5:1441-1448 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 4a	Magalhães A, Rivera C. NKCC1-Deficiency Results in Abnormal Proliferation of Neural Progenitor Cells of the Lateral Ganglionic Eminence. Front Cell Neurosci. 2016;10:200 pubmed publisher
flow cytometry; human; 1:500 immunocytochemistry; human; 1:500; fig s1	Feringa F, Krenning L, Koch A, van den Berg J, van den Broek B, Jalink K, et al. Hypersensitivity to DNA damage in antephase as a safeguard for genome stability. Nat Commun. 2016;7:12618 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:400; fig 5	Hillis J, Davies J, Mundim M, Al Dalahmah O, Szele F. Cuprizone demyelination induces a unique inflammatory response in the subventricular zone. J Neuroinflammation. 2016;13:190 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:800; fig s5	Palibrk V, Suganthan R, Scheffler K, Wang W, BjÃ¸rÃ¥s M, BÃ¸e S. PML regulates neuroprotective innate immunity and neuroblast commitment in a hypoxic-ischemic encephalopathy model. Cell Death Dis. 2016;7:e2320 pubmed publisher
immunocytochemistry; human; 1:800; loading ...; fig 2b	Chen C, Tsao N, Huang L, Yen Y, Liu X, Lehman C, et al. The Impact of dUTPase on Ribonucleotide Reductase-Induced Genome Instability in Cancer Cells. Cell Rep. 2016;16:1287-1299 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; fig 4	Perdigoto C, Dauber K, Bar C, Tsai P, Valdes V, Cohen I, et al. Polycomb-Mediated Repression and Sonic Hedgehog Signaling Interact to Regulate Merkel Cell Specification during Skin Development. PLoS Genet. 2016;12:e1006151 pubmed publisher
immunohistochemistry; mouse; 1:1000; fig 4	Li S, Qu Z, Haas M, Ngo L, Heo Y, Kang H, et al. The HSA21 gene EURL/C21ORF91 controls neurogenesis within the cerebral cortex and is implicated in the pathogenesis of Down Syndrome. Sci Rep. 2016;6:29514 pubmed publisher
immunohistochemistry; Xenopus laevis; 1:500; loading ...; tbl 1	Messina A, Bridi S, Bozza A, Bozzi Y, Baudet M, Casarosa S. Noggin 1 overexpression in retinal progenitors affects bipolar cell generation. Int J Dev Biol. 2016;60:151-7 pubmed publisher
immunocytochemistry; mouse; 1:200; loading ...; fig 2e	Huang Z, Hu J, Pan J, Wang Y, Hu G, Zhou J, et al. YAP stabilizes SMAD1 and promotes BMP2-induced neocortical astrocytic differentiation. Development. 2016;143:2398-409 pubmed publisher
immunocytochemistry; human; loading ...; fig 2h western blot; human; loading ...; fig 2j	Gholkar A, Cheung K, Williams K, Lo Y, Hamideh S, Nnebe C, et al. Fatostatin Inhibits Cancer Cell Proliferation by Affecting Mitotic Microtubule Spindle Assembly and Cell Division. J Biol Chem. 2016;291:17001-8 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 5k	Stock K, Estrada M, Vidic S, Gjerde K, Rudisch A, Santo V, et al. Capturing tumor complexity in vitro: Comparative analysis of 2D and 3D tumor models for drug discovery. Sci Rep. 2016;6:28951 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 1	Fame R, MacDonald J, Dunwoodie S, Takahashi E, Macklis J. Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity. J Neurosci. 2016;36:6403-19 pubmed publisher
immunohistochemistry; mouse; 1:150; loading ...; fig 5a	Parang B, Bradley A, Mittal M, Short S, Thompson J, Barrett C, et al. Myeloid translocation genes differentially regulate colorectal cancer programs. Oncogene. 2016;35:6341-6349 pubmed publisher
western blot; human; 1:1000; fig 4	Barretta M, Spano D, D Ambrosio C, Cervigni R, Scaloni A, Corda D, et al. Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2. Nat Commun. 2016;7:11727 pubmed publisher
western blot; mouse; fig 1	van Ree J, Nam H, Jeganathan K, Kanakkanthara A, van Deursen J. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. Nat Cell Biol. 2016;18:814-21 pubmed publisher
immunocytochemistry; chicken; 1:1000; loading ...; fig 7b	Ohta S, Montano Gutierrez L, de Lima Alves F, Ogawa H, Toramoto I, Sato N, et al. Proteomics Analysis with a Nano Random Forest Approach Reveals Novel Functional Interactions Regulated by SMC Complexes on Mitotic Chromosomes. Mol Cell Proteomics. 2016;15:2802-18 pubmed publisher
western blot; human; fig 6	Rao V, Ow J, Shankar S, Bharathy N, Manikandan J, Wang Y, et al. G9a promotes proliferation and inhibits cell cycle exit during myogenic differentiation. Nucleic Acids Res. 2016;44:8129-43 pubmed publisher
immunohistochemistry - frozen section; zebrafish ; 1:1000; fig 2	Hudish L, Galati D, Ravanelli A, Pearson C, Huang P, Appel B. miR-219 regulates neural progenitors by dampening apical Par protein-dependent Hedgehog signaling. Development. 2016;143:2292-304 pubmed publisher
immunohistochemistry - paraffin section; chicken; 1:300; fig 2	McKey J, Martire D, de Santa Barbara P, Faure S. LIX1 regulates YAP1 activity and controls the proliferation and differentiation of stomach mesenchymal progenitors. BMC Biol. 2016;14:34 pubmed publisher
immunohistochemistry - paraffin section; mouse; 1:150; loading ...; fig 5c	Nakamura R, Koshiba Takeuchi K, Tsuchiya M, Kojima M, Miyazawa A, Ito K, et al. Expression analysis of Baf60c during heart regeneration in axolotls and neonatal mice. Dev Growth Differ. 2016;58:367-82 pubmed publisher
immunohistochemistry; mouse; 1:2000; fig 3k	Emechebe U, Kumar P P, Rozenberg J, Moore B, Firment A, Mirshahi T, et al. T-box3 is a ciliary protein and regulates stability of the Gli3 transcription factor to control digit number. elife. 2016;5: pubmed publisher
immunocytochemistry; mouse; fig 6	Rooney N, Wang P, Brennan K, Gilmore A, Streuli C. The Integrin-Mediated ILK-Parvin-?Pix Signaling Axis Controls Differentiation in Mammary Epithelial Cells. J Cell Physiol. 2016;231:2408-17 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:300; fig s3	Panousopoulou E, Hobbs C, Mason I, Green J, Formstone C. Epiboly generates the epidermal basal monolayer and spreads the nascent mammalian skin to enclose the embryonic body. J Cell Sci. 2016;129:1915-27 pubmed publisher
immunohistochemistry; Holothuria glaberrima; 1:250; fig 3	Díaz Balzac C, Lázaro Peña M, Vázquez Figueroa L, Díaz Balzac R, Garcia Arraras J. Holothurian Nervous System Diversity Revealed by Neuroanatomical Analysis. PLoS ONE. 2016;11:e0151129 pubmed publisher
immunocytochemistry; human; 1:1000	Sagi I, Chia G, Golan Lev T, Peretz M, Weissbein U, Sui L, et al. Derivation and differentiation of haploid human embryonic stem cells. Nature. 2016;532:107-11 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:500; fig 4	Goodliffe J, Olmos Serrano J, Aziz N, Pennings J, Guedj F, Bianchi D, et al. Absence of Prenatal Forebrain Defects in the Dp(16)1Yey/+ Mouse Model of Down Syndrome. J Neurosci. 2016;36:2926-44 pubmed publisher
flow cytometry; human western blot; human	Mitxelena J, Apraiz A, Vallejo RodrÃguez J, Malumbres M, Zubiaga A. E2F7 regulates transcription and maturation of multiple microRNAs to restrain cell proliferation. Nucleic Acids Res. 2016;: pubmed
western blot; human; 1:1000; fig 1	Hein J, Nilsson J. Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry. Nat Commun. 2016;7:10975 pubmed publisher
immunocytochemistry; human; 1:1000; fig 8	Petridou N, Skourides P. A ligand-independent integrin Î²1 mechanosensory complex guides spindle orientation. Nat Commun. 2016;7:10899 pubmed publisher
immunohistochemistry - frozen section; mouse; fig 3	Li D, Sinha T, Ajima R, Seo H, Yamaguchi T, Wang J. Spatial regulation of cell cohesion by Wnt5a during second heart field progenitor deployment. Dev Biol. 2016;412:18-31 pubmed publisher
western blot; human; 1:1000; fig s1	Jia L, Li B, Yu H. The Bub1-Plk1 kinase complex promotes spindle checkpoint signalling through Cdc20 phosphorylation. Nat Commun. 2016;7:10818 pubmed publisher
immunohistochemistry; mouse; 1:100; loading ...; tbl 4	Gonçalves A, Thorsteinsdóttir S, Deries M. Rapid and simple method for in vivo ex utero development of mouse embryo explants. Differentiation. 2016;91:57-67 pubmed publisher
immunohistochemistry; mouse; 1:500; loading ...; fig 3d	Yu W, Huang X, Tian X, Zhang H, He L, Wang Y, et al. GATA4 regulates Fgf16 to promote heart repair after injury. Development. 2016;143:936-49 pubmed publisher
immunohistochemistry; Xenopus laevis; 1 ug/ml; loading ...; fig 5a	Devotta A, Juraver Geslin H, Gonzalez J, Hong C, Saint Jeannet J. Sf3b4-depleted Xenopus embryos: A model to study the pathogenesis of craniofacial defects in Nager syndrome. Dev Biol. 2016;415:371-382 pubmed publisher
immunohistochemistry; human; 1:500; fig 1	Lamba Saini M, Bouzin C, Weynand B, Marbaix E. An Appraisal of Proliferation and Apoptotic Markers in Papillary Thyroid Carcinoma: An Automated Analysis. PLoS ONE. 2016;11:e0148656 pubmed publisher
immunohistochemistry; chicken; 1:200; fig 2	Long K, Moss L, Laursen L, Boulter L, ffrench Constant C. Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. Nat Commun. 2016;7:10354 pubmed publisher
immunocytochemistry; mouse; fig 3	Iyer S, Chou F, Wang R, Chiu H, Raju V, Little M, et al. Crim1 has cell-autonomous and paracrine roles during embryonic heart development. Sci Rep. 2016;6:19832 pubmed publisher
immunocytochemistry; human; 1:1000; fig 5b	Walter D, Hoffmann S, Komseli E, Rappsilber J, Gorgoulis V, SÃ¸rensen C. SCF(Cyclin F)-dependent degradation of CDC6 suppresses DNA re-replication. Nat Commun. 2016;7:10530 pubmed publisher
flow cytometry; human; fig s2	Moudry P, Watanabe K, Wolanin K, Bartkova J, Wassing I, Watanabe S, et al. TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. J Cell Biol. 2016;212:281-8 pubmed publisher
flow cytometry; human; fig 2	Hasvold G, Lund Andersen C, Lando M, Patzke S, Hauge S, Suo Z, et al. Hypoxia-induced alterations of G2 checkpoint regulators. Mol Oncol. 2016;10:764-73 pubmed publisher
western blot; human; fig 1	Kuo C, Li X, Stark J, Shih H, Ann D. RNF4 regulates DNA double-strand break repair in a cell cycle-dependent manner. Cell Cycle. 2016;15:787-98 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:100; fig 2	Wilhelm K, Happel K, Eelen G, Schoors S, Oellerich M, Lim R, et al. FOXO1 couples metabolic activity and growth state in the vascular endothelium. Nature. 2016;529:216-20 pubmed publisher
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product information

Catalog Number :

06-570

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

phospho-Histone H3 (Ser10)

Conjugate :

Purified

Product Description :

Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker

Cross Reactivity :

Mouse;Human

Background :

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

ALT Names :

H3S10P;Histone H3 (phospho S10);H3 histone, family 3A;H3 histone, family 3B;H3 histone, family 3B (H3.3B)

Immunogen :

Linear peptide corresponding to human Histone H3 at Ser10.

Specificity :

This antibody recognizes Histone H3 when phosphorylated at Ser10.

Package Size :

200 µg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

2-8°C for up to 6 months.