FAK Antibody | EMD Millipore 06-543 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

FAK Antibody

catalog :

06-543

quantity :

200 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 2c western blot; human; 1:1000; loading ...; fig 4b, 6s1c	Diaz Osterman C, Ozmadenci D, Kleinschmidt E, Taylor K, Barrie A, Jiang S, et al. FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy. elife. 2019;8: pubmed publisher
immunohistochemistry - frozen section; mouse; loading ...; fig 3c	Yui S, Azzolin L, Maimets M, Pedersen M, Fordham R, Hansen S, et al. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Cell Stem Cell. 2018;22:35-49.e7 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 3c	Benito JardÃ³n M, Klapproth S, Gimeno LLuch I, Petzold T, Bharadwaj M, MÃ¼ller D, et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. elife. 2017;6: pubmed publisher
western blot; mouse; 1:1000; fig 5	Theodosiou M, Widmaier M, BÃ¶ttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
	Heim J, Squirewell E, Neu A, Zocher G, Sominidi Damodaran S, Wyles S, et al. Myosin-1E interacts with FAK proline-rich region 1 to induce fibronectin-type matrix. Proc Natl Acad Sci U S A. 2017;114:3933-3938 pubmed publisher

product information

Catalog Number :

06-543

Subcategory :

Cell Signaling

Product Name :

Anti-FAK Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q05397

Host Name :

Rabbit

Antigen :

FAK

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-FAK Antibody

Cross Reactivity :

Human;Mouse;Rat;Hamster

Background :

Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and it's phosphorylation states have been implicated in cancer metastasis and tumor cell survival and adhesion-independent growth. Additionally, recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.

ALT Names :

FADK 1;PTK2 protein tyrosine kinase 2;Protein-tyrosine kinase 2;focal adhesion kinase 1

Immunogen :

pGEX-derived fusion protein containing residues 748-1053 of human FAK

Specificity :

Recognizes and is specific for human p125FAK, does not cross-react with Pyk2.

Package Size :

200 μg

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry

Storage :

Stable for 1 year at from date of receipt.