Bak Antibody, NT | EMD Millipore 06-536 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Bak Antibody, NT

catalog :

06-536

quantity :

200 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 2a	Suzuki J, Nakajima W, Suzuki H, Asano Y, Tanaka N. Chaperone-mediated autophagy promotes lung cancer cell survival through selective stabilization of the pro-survival protein, MCL1. Biochem Biophys Res Commun. 2017;482:1334-1340 pubmed publisher
western blot; human; loading ...; fig 1a	Nakajima W, Sharma K, Lee J, Maxim N, Hicks M, Vu T, et al. DNA damaging agent-induced apoptosis is regulated by MCL-1 phosphorylation and degradation mediated by the Noxa/MCL-1/CDK2 complex. Oncotarget. 2016;7:36353-36365 pubmed publisher
western blot; human; loading ...; fig 3e	Matsumoto M, Nakajima W, Seike M, Gemma A, Tanaka N. Cisplatin-induced apoptosis in non-small-cell lung cancer cells is dependent on Bax- and Bak-induction pathway and synergistically activated by BH3-mimetic ABT-263 in p53 wild-type and mutant cells. Biochem Biophys Res Commun. 2016;473:490-6 pubmed publisher
western blot; human; loading ...; fig 1	Nakajima W, Sharma K, Hicks M, Le N, Brown R, Krystal G, et al. Combination with vorinostat overcomes ABT-263 (navitoclax) resistance of small cell lung cancer. Cancer Biol Ther. 2016;17:27-35 pubmed publisher
	Schulman J, Szczesniak L, Bunker E, Nelson H, Roe M, Wagner L, et al. Bok regulates mitochondrial fusion and morphology. Cell Death Differ. 2019;: pubmed publisher
	Wei Q, Sun H, Song S, Liu Y, Liu P, Livingston M, et al. MicroRNA-668 represses MTP18 to preserve mitochondrial dynamics in ischemic acute kidney injury. J Clin Invest. 2018;128:5448-5464 pubmed publisher
	Singh P, Roukounakis A, Frank D, Kirschnek S, Das K, Neumann S, et al. Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis. Genes Dev. 2017;31:1754-1769 pubmed publisher
	Kawakami H, Huang S, Pal K, Dutta S, Mukhopadhyay D, Sinicrope F. Mutant BRAF Upregulates MCL-1 to Confer Apoptosis Resistance that Is Reversed by MCL-1 Antagonism and Cobimetinib in Colorectal Cancer. Mol Cancer Ther. 2016;15:3015-3027 pubmed
	Sun J, Mao L, Polonsky K, Ren D. Pancreatic ?-Cell Death due to Pdx-1 Deficiency Requires Multi-BH Domain Protein Bax but Not Bak. J Biol Chem. 2016;291:13529-34 pubmed publisher

product information

Catalog Number :

06-536

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-Bak Antibody, NT

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q16611

Host Name :

Rabbit

Antigen :

Bak

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-Bak Antibody, NT

Cross Reactivity :

Human;Rat;Mouse

Background :

Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.

ALT Names :

Apoptosis regulator BAK;BCL2-antagonist/killer 1;BCL2-like 7 protein;Bcl-2 homologous antagonist/killer;Bcl-2-like 7 protein;pro-apoptotic protein BAK

Immunogen :

Synthetic peptide (SEEQVAQQDTEEVFRSC) corresponding to amino acid residues 23-38 of human Bak with a cysteine residue added to the C-terminus for conjugation to KLH.

Specificity :

Specific for Bak, MW ~28 kDa.

Package Size :

200 μg

Uses :

Immunocytochemistry;Immunohistochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt.