phospho-CREB (Ser133) Antibody | EMD Millipore 06-519 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-CREB (Ser133) Antibody

catalog :

06-519

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, EMSA, chromatin immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section, immunohistochemistry - free floating section, ChIP-Seq

citations: 17

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; human; loading ...; fig 9	Meyer M, Benkusky N, Kaufmann M, Lee S, Redfield R, Jones G, et al. Targeted genomic deletions identify diverse enhancer functions and generate a kidney-specific, endocrine-deficient Cyp27b1 pseudo-null mouse. J Biol Chem. 2019;294:9518-9535 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 5a	Jilg A, Bechstein P, Saade A, Dick M, Li T, Tosini G, et al. Melatonin modulates daytime-dependent synaptic plasticity and learning efficiency. J Pineal Res. 2019;66:e12553 pubmed publisher
ChIP-Seq; mouse; loading ...; fig 7b	Meyer M, Benkusky N, Kaufmann M, Lee S, Onal M, Jones G, et al. A kidney-specific genetic control module in mice governs endocrine regulation of the cytochrome P450 gene Cyp27b1 essential for vitamin D3 activation. J Biol Chem. 2017;292:17541-17558 pubmed publisher
immunohistochemistry - free floating section; mouse; 1:5000; loading ...; fig s3f	Castellano J, Mosher K, Abbey R, McBride A, James M, Berdnik D, et al. Human umbilical cord plasma proteins revitalize hippocampal function in aged mice. Nature. 2017;544:488-492 pubmed publisher
western blot; mouse; loading ...; fig 7f	Cong Q, Jia H, Li P, Qiu S, Yeh J, Wang Y, et al. p38α MAPK regulates proliferation and differentiation of osteoclast progenitors and bone remodeling in an aging-dependent manner. Sci Rep. 2017;7:45964 pubmed publisher
chromatin immunoprecipitation; mouse; loading ...; fig 4e	Mathew R, Tatarakis A, Rudenko A, Johnson Venkatesh E, Yang Y, Murphy E, et al. A microRNA negative feedback loop downregulates vesicle transport and inhibits fear memory. elife. 2016;5: pubmed publisher
immunocytochemistry; rat; 1:1000; loading ...; fig 5a	Olianas M, Dedoni S, Onali P. LPA1 Mediates Antidepressant-Induced ERK1/2 Signaling and Protection from Oxidative Stress in Glial Cells. J Pharmacol Exp Ther. 2016;359:340-353 pubmed
western blot; mouse; loading ...; fig s2	Inda C, Dos Santos Claro P, Bonfiglio J, Senin S, Maccarrone G, Turck C, et al. Different cAMP sources are critically involved in G protein-coupled receptor CRHR1 signaling. J Cell Biol. 2016;214:181-95 pubmed publisher
western blot; rat; 1:1000; loading ...; fig 7e	Alba Delgado C, Cebada Aleu A, Mico J, Berrocoso E. Comorbid anxiety-like behavior and locus coeruleus impairment in diabetic peripheral neuropathy: A comparative study with the chronic constriction injury model. Prog Neuropsychopharmacol Biol Psychiatry. 2016;71:45-56 pubmed publisher
	Meyer M, Lee S, Carlson A, Benkusky N, Kaufmann M, Jones G, et al. A chromatin-based mechanism controls differential regulation of the cytochrome P450 gene Cyp24a1 in renal and non-renal tissues. J Biol Chem. 2019;294:14467-14481 pubmed publisher
	Pluvinage J, Haney M, Smith B, Sun J, Iram T, Bonanno L, et al. CD22 blockade restores homeostatic microglial phagocytosis in ageing brains. Nature. 2019;568:187-192 pubmed publisher
	Egan A, Thompson A, Buesing D, Fourman S, Packard A, Terefe T, et al. Palatable Food Affects HPA Axis Responsivity and Forebrain Neurocircuitry in an Estrous Cycle-specific Manner in Female Rats. Neuroscience. 2018;384:224-240 pubmed publisher
	Shang Y, Zhang J, Huang E. HIPK2-Mediated Transcriptional Control of NMDA Receptor Subunit Expression Regulates Neuronal Survival and Cell Death. J Neurosci. 2018;38:4006-4019 pubmed publisher
	Pahng A, Paulsen R, McGinn M, Edwards K, Edwards S. Neurobiological Correlates of Pain Avoidance-Like Behavior in Morphine-Dependent and Non-Dependent Rats. Neuroscience. 2017;366:1-14 pubmed publisher
	Zhang Z, Newton K, Kummerfeld S, Webster J, Kirkpatrick D, Phu L, et al. Transcription factor Etv5 is essential for the maintenance of alveolar type II cells. Proc Natl Acad Sci U S A. 2017;114:3903-3908 pubmed publisher
	OrtuÃ±o M, Robinson S, Subramanyam P, Paone R, Huang Y, Guo X, et al. Serotonin-reuptake inhibitors act centrally to cause bone loss in mice by counteracting a local anti-resorptive effect. Nat Med. 2016;22:1170-1179 pubmed publisher
	Yiu A, Rashid A, Josselyn S. Increasing CREB function in the CA1 region of dorsal hippocampus rescues the spatial memory deficits in a mouse model of Alzheimer's disease. Neuropsychopharmacology. 2011;36:2169-86 pubmed publisher

product information

Catalog Number :

06-519

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-CREB (Ser133) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P16220

Host Name :

Rabbit

Antigen :

phospho-CREB (Ser133)

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-phospho-CREB (Ser133) Antibody

Cross Reactivity :

Human;Rat;Mouse;Hamster

Background :

CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.

ALT Names :

active transcription factor CREB;cAMP responsive element binding protein 1;cAMP-response element-binding protein-1;transactivator protein

Immunogen :

KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.

Specificity :

Recognizes p43 phosphorylated CREB. May also recognize p30 and p38 kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.

Package Size :

100 µL

Uses :

Electrophoretic Mobility Shift Assay;Immunohistochemistry;Immunohistochemistry (Paraffin);Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.