Caspase 1 Antibody | EMD Millipore 06-503 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Caspase 1 Antibody

catalog :

06-503

quantity :

200 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; fig 2	Wang X, Shaw D, Hammond H, Sutterwala F, Rayamajhi M, Shirey K, et al. The Prostaglandin E2-EP3 Receptor Axis Regulates Anaplasma phagocytophilum-Mediated NLRC4 Inflammasome Activation. PLoS Pathog. 2016;12:e1005803 pubmed publisher
western blot; mouse; 1:1000; fig 1	Wang X, Shaw D, Sakhon O, Snyder G, Sundberg E, Santambrogio L, et al. The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation. Infect Immun. 2016;84:1796-1805 pubmed publisher

product information

Catalog Number :

06-503

Subcategory :

Apoptosis & Cancer

Product Name :

Anti-Caspase 1 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P29466

Host Name :

Rabbit

Antigen :

Caspase 1

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-Caspase 1 Antibody

Cross Reactivity :

Human;Rat;Mouse

Background :

Caspase-1, also known as IL-1 beta-converting enzyme (ICE), is an ezyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides. Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis. Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C. elegans. Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). Caspase-1 is highly expressed in spleen and lung, and is absent from brain.

ALT Names :

CASP1 nirs variant 1;IL-1 beta-converting enzyme;Interleukin-1 beta convertase;Interleukin-1 beta-converting enzyme;caspase 1;caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase);caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase);interleukin 1-B converting enzyme;interleukin 1-beta convertase

Immunogen :

KLH-conjugated, synthetic peptide corresponding to amino acid residues 129-149 of human Caspase 1 (PEHKTSDSTFLVFMSHGIREG).

Specificity :

Recognizes the p45 proenzyme, Mr 45 kDa, and the p20 subunit, Mr 20 kDa, of reduced human Caspase 1 isoforms.

Package Size :

200 µg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.