Phosphotyrosine Antibody | EMD Millipore 06-427 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Phosphotyrosine Antibody

catalog :

06-427

quantity :

200 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot, immunoprecipitation

citations: 1

Reference
Hwang C, Wagley Y, Law P, Wei L, Loh H. Phosphorylation of poly(rC) binding protein 1 (PCBP1) contributes to stabilization of mu opioid receptor (MOR) mRNA via interaction with AU-rich element RNA-binding protein 1 (AUF1) and poly A binding protein (PABP). Gene. 2017;598:113-130 pubmed publisher

product information

Catalog Number :

06-427

Subcategory :

Cell Signaling

Product Name :

Anti-Phosphotyrosine Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Host Name :

Rabbit

Antigen :

Phosphotyrosine

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-Phosphotyrosine Antibody

Cross Reactivity :

Human;Rat;Mouse

Background :

The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.

Immunogen :

In order to produce broad spectrum polyclonal phosphotyrosine antibodies, rabbits were immunized with three phosphorylated immunogens: (1) phosphotyrosine covalently linked to KLH; (2) the c-Src carboxyl terminal regulatory phosphopeptide (TS-T-E-P-Q-pY-Q-P-G-E-N-L; Catalog # 12-218) covalently linked to KLH, and; (3) a phosphopeptide associated with high tyrosine kinase activity in human lymphocytes (R-R-L-I-E-D-A-E-pY-A-A-R-G; Catalog # 12-217) covalently linked to KLH. Both of the phosphopeptide haptens serve as strong substrates for tyrosine phosphatases and are part of the two colorimetric tyrosine phosphatase kits provided by Upstate, Inc. (Catalog # 17-125, 17-126).

Specificity :

The immunoreactivity of the antibody is totally inhibited by the use of 100 mM phenyl phosphate, a phosphotyrosine analog. The phosphotyrosine antibody is purified by immunoaffinity chromatography using either a dual phospho-peptide gel or a BSA-phosphotyrosine gel. All of the phosphotyrosine immunoreactivity present in the antisera is immunoadsorbed whether the antibody is purified by either gel indicating that the antibody is not sequence-specific but specific for phosphotyrosine residues.

Package Size :

200 μg

Uses :

Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt.