NFκB p65 Antibody, CT | EMD Millipore 06-418 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

NFκB p65 Antibody, CT

catalog :

06-418

quantity :

100 µg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunoprecipitation, EMSA, chromatin immunoprecipitation

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 7a	Ma J, Sanchez B, Hall D, Tremblay A, Di Marco S, Gallouzi I. STAT3 promotes IFNÎ³/TNFÎ±-induced muscle wasting in an NF-ÎºB-dependent and IL-6-independent manner. EMBO Mol Med. 2017;9:622-637 pubmed publisher
chromatin immunoprecipitation; human; fig 3b western blot; human; 1:1000; fig 2a	Harris D, Chandrasekharan U, Bandyopadhyay S, Willard B, DiCorleto P. PRMT5-Mediated Methylation of NF-ÎºB p65 at Arg174 Is Required for Endothelial CXCL11 Gene Induction in Response to TNF-Î± and IFN-Î³ Costimulation. PLoS ONE. 2016;11:e0148905 pubmed publisher
	Gari H, Degala G, Lucia M, Lambert J. Loss of the oncogenic phosphatase PRL-3 promotes a TNF-R1 feedback loop that mediates triple-negative breast cancer growth. Oncogenesis. 2016;5:e255 pubmed publisher
	Sun S, Wu C, Sheu G, Chang H, Chen M, Lin Y, et al. Integrin ?3 and CD44 levels determine the effects of the OPN-a splicing variant on lung cancer cell growth. Oncotarget. 2016;7:55572-55584 pubmed publisher

product information

Catalog Number :

06-418

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-NFκB p65 Antibody, CT

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

Q04206

Host Name :

Rabbit

Antigen :

NFκB p65

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-NFκB p65 Antibody, CT

Cross Reactivity :

Human;Rat;Mouse

Background :

The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.

ALT Names :

Nuclear factor NF-kappa-B p65 subunit;v;v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65));v-rel reticuloendotheliosis viral oncogene homolog A (avian);v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65;v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65 (avian)

Immunogen :

KLH-conjugated, synthetic peptide corresponding to the 13 C-terminal amino acids (C-DMDFSALLSQISS) of the p65 subunit of human NFκB containing an N-terminal cysteine for conjugation. The immunizing sequence is identical in mouse.

Specificity :

NFκB p65, Mr 65 kDa

Package Size :

100 µg

Uses :

Electrophoretic Mobility Shift Assay;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.