IRS1 Antibody | EMD Millipore 06-248 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

IRS1 Antibody

catalog :

06-248

quantity :

100 μg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, reverse phase protein lysate microarray, other, western blot knockout validation

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:2000; loading ...; fig 7a	Kubota N, Kubota T, Kajiwara E, Iwamura T, Kumagai H, Watanabe T, et al. Differential hepatic distribution of insulin receptor substrates causes selective insulin resistance in diabetes and obesity. Nat Commun. 2016;7:12977 pubmed publisher
other; human; loading ...; fig 4c	Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig st6	Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig 3a	. Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
western blot; human; loading ...	Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
western blot; mouse; loading ...; fig 4i	Wang X, Cao Q, Yu L, Shi H, Xue B, Shi H. Epigenetic regulation of macrophage polarization and inflammation by DNA methylation in obesity. JCI Insight. 2016;1:e87748 pubmed publisher
	Lassak A, Dean M, Wyczechowska D, Wilk A, Marrero L, Trillo Tinoco J, et al. Molecular and Structural Traits of Insulin Receptor Substrate 1/LC3 Nuclear Structures and Their Role in Autophagy Control and Tumor Cell Survival. Mol Cell Biol. 2018;38: pubmed publisher
	Irimia J, Meyer C, Segvich D, Surendran S, DePaoli Roach A, Morral N, et al. Lack of liver glycogen causes hepatic insulin resistance and steatosis in mice. J Biol Chem. 2017;292:10455-10464 pubmed publisher
	Parker L, Trewin A, Levinger I, Shaw C, Stepto N. The effect of exercise-intensity on skeletal muscle stress kinase and insulin protein signaling. PLoS ONE. 2017;12:e0171613 pubmed publisher
	Chawla B, Hedman A, Sayedyahossein S, Erdemir H, Li Z, Sacks D. Absence of IQGAP1 Protein Leads to Insulin Resistance. J Biol Chem. 2017;292:3273-3289 pubmed publisher
	Farabaugh S, Chan B, Cui X, Dearth R, Lee A. Lack of interaction between ErbB2 and insulin receptor substrate signaling in breast cancer. Cell Commun Signal. 2016;14:25 pubmed
	Santacatterina F, SÃ¡nchez Cenizo L, Formentini L, Mobasher M, Casas E, Rueda C, et al. Down-regulation of oxidative phosphorylation in the liver by expression of the ATPase inhibitory factor 1 induces a tumor-promoter metabolic state. Oncotarget. 2016;7:490-508 pubmed publisher

product information

Catalog Number :

06-248

Subcategory :

Cell Signaling

Product Name :

Anti-IRS1 Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P35568

Host Name :

Rabbit

Antigen :

IRS1

Conjugate :

Affinity Purified

Isotype :

IgG

Product Description :

Anti-IRS1 Antibody

Cross Reactivity :

Human;Mouse;Rat

Background :

Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

Immunogen :

Carboxy-terminal 14 amino acid peptide ([C]YASINFQKQPEDRQ) of rat liver IRS1

Specificity :

Recognizes IRS1, Mr 160-185kDa.

Package Size :

100 μg

Uses :

Immunoprecipitation;Western Blotting

Storage :

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.