phospho-erbB-2/HER-2 (Tyr1248) Antibody | EMD Millipore 06-229 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-erbB-2/HER-2 (Tyr1248) Antibody

catalog :

06-229

quantity :

100 µL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human

application :

western blot, immunocytochemistry, reverse phase protein lysate microarray

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig s10	Grasso S, Chapelle J, Salemme V, Aramu S, Russo I, Vitale N, et al. The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries. Nat Commun. 2017;8:14797 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig st6	Cherniack A, Shen H, Walter V, Stewart C, Murray B, Bowlby R, et al. Integrated Molecular Characterization of Uterine Carcinosarcoma. Cancer Cell. 2017;31:411-423 pubmed publisher
reverse phase protein lysate microarray; human; loading ...; fig 3a	. Integrated genomic and molecular characterization of cervical cancer. Nature. 2017;543:378-384 pubmed publisher
western blot; human; loading ...	Hill S, Nesser N, Johnson Camacho K, Jeffress M, Johnson A, Boniface C, et al. Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling. Cell Syst. 2017;4:73-83.e10 pubmed publisher
	Elster N, Toomey S, Fan Y, Cremona M, Morgan C, Weiner Gorzel K, et al. Frequency, impact and a preclinical study of novel ERBB gene family mutations in HER2-positive breast cancer. Ther Adv Med Oncol. 2018;10:1758835918778297 pubmed publisher
	Daemen A, Manning G. HER2 is not a cancer subtype but rather a pan-cancer event and is highly enriched in AR-driven breast tumors. Breast Cancer Res. 2018;20:8 pubmed publisher
	Huang K, Li S, Mertins P, Cao S, Gunawardena H, Ruggles K, et al. Proteogenomic integration reveals therapeutic targets in breast cancer xenografts. Nat Commun. 2017;8:14864 pubmed publisher

product information

Catalog Number :

06-229

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody

Product Type :

Antibodies

Clonality :

Polyclonal Antibody

Gene ID :

P04626

Host Name :

Rabbit

Antigen :

phospho-erbB-2/HER-2 (Tyr1248)

Conjugate :

Affinity Purified

Isotype :

IgG

Product Description :

Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody

Cross Reactivity :

Human;Rat

Background :

The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.

ALT Names :

CD340 antigen;MLN 19;NEU proto-oncogene;Tyrosine kinase-type cell surface receptor HER2;c-erb B2/neu protein;neuroblastoma/glioblastoma derived oncogene homolog;v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog);v-erb-b2 erythroblastic leukemia viral oncogene homolog 2,neuro/glioblastoma derived oncogene homolog (avian)

Immunogen :

Synthetic phosphopeptide, residues 1243-1255, corresponding to a major auto-phosphorylation (Tyr1248) site of human erbB-2/HER-2 (C-AENPEpYLGLDVPV).

Specificity :

Recognizes and is specific for the activated form of erbB-2/HER-2 oncoprotein, MW ~138 kDa.

Package Size :

100 µL

Uses :

Immunocytochemistry;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.