RNA Polymerase II Antibody, CTD, clone 8WG16 | EMD Millipore 05-952 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RNA Polymerase II Antibody, CTD, clone 8WG16

catalog :

05-952

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

8WG16

The same clone is also sold as:

BioLegend: 664914, 664913, 664908, 664906, 664915, 664912, 664916, 664911
Invitrogen: MA1-26249, MA1-10882
Abcam: ab817
Santa Cruz Biotechnology: sc-56767

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 33

Published Application/Species/Sample/Dilution	Reference
chromatin immunoprecipitation; human; loading ...; fig 10e	Achuthan A, Cook A, Lee M, Saleh R, Khiew H, Chang M, et al. Granulocyte macrophage colony-stimulating factor induces CCL17 production via IRF4 to mediate inflammation. J Clin Invest. 2016;126:3453-66 pubmed publisher
ChIP-Seq; mouse; fig 3a	Sin H, Kartashov A, Hasegawa K, Barski A, Namekawa S. Poised chromatin and bivalent domains facilitate the mitosis-to-meiosis transition in the male germline. BMC Biol. 2015;13:53 pubmed publisher
immunoprecipitation; human; 40 ug	Quan J, Adelmant G, Marto J, Look A, Yusufzai T. The chromatin remodeling factor CHD5 is a transcriptional repressor of WEE1. PLoS ONE. 2014;9:e108066 pubmed publisher
chromatin immunoprecipitation; mouse	Kuwahara M, Suzuki J, Tofukuji S, Yamada T, Kanoh M, Matsumoto A, et al. The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis. Nat Commun. 2014;5:3555 pubmed publisher
western blot; mouse	Vella K, Ramadoss P, Costa e Sousa R, Astapova I, Ye F, Holtz K, et al. Thyroid hormone signaling in vivo requires a balance between coactivators and corepressors. Mol Cell Biol. 2014;34:1564-75 pubmed publisher
	Abe H, Alavattam K, Hu Y, Pang Q, Andreassen P, Hegde R, et al. The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis. Curr Biol. 2019;: pubmed publisher
	Gopalakrishnan R, Marr S, Kingston R, Winston F. A conserved genetic interaction between Spt6 and Set2 regulates H3K36 methylation. Nucleic Acids Res. 2019;47:3888-3903 pubmed publisher
	Castellaro A, Rodriguez Baili M, Di Tada C, Gil G. Tumor-Associated Macrophages Induce Endocrine Therapy Resistance in ER+ Breast Cancer Cells. Cancers (Basel). 2019;11: pubmed publisher
	Dunker W, Song Y, Zhao Y, Karijolich J. FUS Negatively Regulates Kaposi's Sarcoma-Associated Herpesvirus Gene Expression. Viruses. 2018;10: pubmed publisher
	Rozenberg J, Taylor J, Mack C. RBPJ binds to consensus and methylated cis elements within phased nucleosomes and controls gene expression in human aortic smooth muscle cells in cooperation with SRF. Nucleic Acids Res. 2018;46:8232-8244 pubmed publisher
	Watts B, Wittmann S, Wery M, Gautier C, Kus K, Birot A, et al. Histone deacetylation promotes transcriptional silencing at facultative heterochromatin. Nucleic Acids Res. 2018;46:5426-5440 pubmed publisher
	Mo X, Zhang H, Preston S, Martin K, Zhou B, Vadalia N, et al. Interferon-γ Signaling in Melanocytes and Melanoma Cells Regulates Expression of CTLA-4. Cancer Res. 2018;78:436-450 pubmed publisher
	Hang S, Gergen J. Different modes of enhancer-specific regulation by Runt and Even-skipped during Drosophila segmentation. Mol Biol Cell. 2017;28:681-691 pubmed publisher
	Tran P, Kennedy B, Lien Y, Simmons R, Georgieff M. Fetal iron deficiency induces chromatin remodeling at the Bdnf locus in adult rat hippocampus. Am J Physiol Regul Integr Comp Physiol. 2015;308:R276-82 pubmed publisher
	Yang S, Li W, Qi S, Gai K, Chen Y, Suo J, et al. The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region. Nucleic Acids Res. 2014;42:6183-95 pubmed publisher
	Hérissant L, Moehle E, Bertaccini D, Van Dorsselaer A, Schaeffer Reiss C, Guthrie C, et al. H2B ubiquitylation modulates spliceosome assembly and function in budding yeast. Biol Cell. 2014;106:126-38 pubmed publisher
	Lenstra T, Tudek A, Clauder S, Xu Z, Pachis S, van Leenen D, et al. The role of Ctk1 kinase in termination of small non-coding RNAs. PLoS ONE. 2013;8:e80495 pubmed publisher
	Zhang Y, Vastenhouw N, Feng J, Fu K, Wang C, Ge Y, et al. Canonical nucleosome organization at promoters forms during genome activation. Genome Res. 2014;24:260-6 pubmed publisher
	Artal Martinez de Narvajas A, Gomez T, Zhang J, Mann A, Taoda Y, Gorman J, et al. Epigenetic regulation of autophagy by the methyltransferase G9a. Mol Cell Biol. 2013;33:3983-93 pubmed publisher
	Mukundan B, Ansari A. Srb5/Med18-mediated termination of transcription is dependent on gene looping. J Biol Chem. 2013;288:11384-94 pubmed publisher
	Lim C, Reversade B, Knowles B, Solter D. Optimal histone H3 to linker histone H1 chromatin ratio is vital for mesodermal competence in Xenopus. Development. 2013;140:853-60 pubmed publisher
	Endoh M, Endo T, Endoh T, Isono K, Sharif J, Ohara O, et al. Histone H2A mono-ubiquitination is a crucial step to mediate PRC1-dependent repression of developmental genes to maintain ES cell identity. PLoS Genet. 2012;8:e1002774 pubmed publisher
	Devaiah B, Lewis B, Cherman N, Hewitt M, Albrecht B, Robey P, et al. BRD4 is an atypical kinase that phosphorylates serine2 of the RNA polymerase II carboxy-terminal domain. Proc Natl Acad Sci U S A. 2012;109:6927-32 pubmed publisher
	St Amour C, Sansó M, Bösken C, Lee K, Larochelle S, Zhang C, et al. Separate domains of fission yeast Cdk9 (P-TEFb) are required for capping enzyme recruitment and primed (Ser7-phosphorylated) Rpb1 carboxyl-terminal domain substrate recognition. Mol Cell Biol. 2012;32:2372-83 pubmed publisher
	Ni Z, Olsen J, Guo X, Zhong G, Ruan E, Marcon E, et al. Control of the RNA polymerase II phosphorylation state in promoter regions by CTD interaction domain-containing proteins RPRD1A and RPRD1B. Transcription. 2011;2:237-42 pubmed publisher
	Hwang J, Saffert R, Kalejta R. Elongin B-mediated epigenetic alteration of viral chromatin correlates with efficient human cytomegalovirus gene expression and replication. MBio. 2011;2:e00023-11 pubmed publisher
	Zediak V, Johnnidis J, Wherry E, Berger S. Cutting edge: persistently open chromatin at effector gene loci in resting memory CD8+ T cells independent of transcriptional status. J Immunol. 2011;186:2705-9 pubmed publisher
	Garcia A, Rosonina E, Manley J, Calvo O. Sub1 globally regulates RNA polymerase II C-terminal domain phosphorylation. Mol Cell Biol. 2010;30:5180-93 pubmed publisher
	Reynoird N, Schwartz B, Delvecchio M, Sadoul K, Meyers D, Mukherjee C, et al. Oncogenesis by sequestration of CBP/p300 in transcriptionally inactive hyperacetylated chromatin domains. EMBO J. 2010;29:2943-52 pubmed publisher
	Alder O, Lavial F, Helness A, Brookes E, Pinho S, Chandrashekran A, et al. Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment. Development. 2010;137:2483-92 pubmed publisher
	van der Knaap J, Kozhevnikova E, Langenberg K, Moshkin Y, Verrijzer C. Biosynthetic enzyme GMP synthetase cooperates with ubiquitin-specific protease 7 in transcriptional regulation of ecdysteroid target genes. Mol Cell Biol. 2010;30:736-44 pubmed publisher
	Malik S, Jiang S, Garee J, Verdin E, Lee A, O Malley B, et al. Histone deacetylase 7 and FoxA1 in estrogen-mediated repression of RPRM. Mol Cell Biol. 2010;30:399-412 pubmed publisher
	Spencer C, Kruhlak M, Jenkins H, Sun X, Bazett Jones D. Mitotic transcription repression in vivo in the absence of nucleosomal chromatin condensation. J Cell Biol. 2000;150:13-26 pubmed

product information

Catalog Number :

05-952

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-RNA Polymerase II Antibody, CTD, clone 8WG16

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P24928

Host Name :

Mouse

Antigen :

RNA Polymerase II

Clone :

8WG16

Conjugate :

Ascites

Product Description :

Anti-RNA Polymerase II Antibody, CTD, clone 8WG16

Cross Reactivity :

Human;Yeast ( S. cerevisiae )

Background :

As the study of the RNA polymerase II (RNAPII) transcription complex expands, many researchers are interested in using immunoblots to detect RNAPII in various protein preparations and column fractions. HeLa cell RNAPII contains 10 subunits, the largest possessing a heptapeptide repeat (PTSPSYS) in the C-terminal domain (CTD). This large subunit cycles, during the course of transcription, through nonphosphorylated (RNAPIIA) and hyperphosphorylated (RNAPIIO) forms. RNAPIIA associates with the basal transcription complex and becomes phosphorylated to the RNAPIIO form before the synthesis of the first phosphodiester bond. The timing of these events suggests that this phosphorylation event may function as a regulatory point, and there is considerable interest in identifying components of the transcription complex with CTD kinase activity

ALT Names :

DNA directed RNA polymerase II polypeptide A;DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA polymerase II subunit B1;polymerase (RNA) II (DNA directed) polypeptide A (220kD);polymerase (RNA) II (DNA directed) polypeptide A, 220kDa

Immunogen :

RNA Polymerase II from wheat germ extract. Clone 8WG16.

Specificity :

Recognizes RNA Polymerase II, Mr 220 kDa.

Package Size :

100 µL

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.