Histidine Tagged Antibody, clone HIS.H8 | EMD Millipore 05-949 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Histidine Tagged Antibody, clone HIS.H8

catalog :

05-949

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

HIS.H8

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 9

Reference
Wood N, Chung K, Blocker A, Rodrigues de Almeida N, Conda Sheridan M, Fisher D, et al. Initial Characterization of the Two ClpP Paralogs of Chlamydia trachomatis Suggests Unique Functionality for Each. J Bacteriol. 2019;201: pubmed publisher
Winer B, Huang T, Pludwinski E, Heller B, Wojcik F, Lipkowitz G, et al. Long-term hepatitis B infection in a scalable hepatic co-culture system. Nat Commun. 2017;8:125 pubmed publisher
Gama J, Pereira C, SimÃµes P, Celestino R, Reis R, Barbosa D, et al. Molecular mechanism of dynein recruitment to kinetochores by the Rod-Zw10-Zwilch complex and Spindly. J Cell Biol. 2017;216:943-960 pubmed publisher
Chua C, Sam I, Chiam C, Chan Y. The neutralizing role of IgM during early Chikungunya virus infection. PLoS ONE. 2017;12:e0171989 pubmed publisher
Beuret N, Hasler F, Prescianotto Baschong C, Birk J, Rutishauser J, Spiess M. Amyloid-like aggregation of provasopressin in diabetes insipidus and secretory granule sorting. BMC Biol. 2017;15:5 pubmed publisher
Kim D, Yeom J, Lee B, Lee K, Bae J, Rhee S. Inhibition of discoidin domain receptor 2-mediated lung cancer cells progression by gold nanoparticle-aptamer-assisted delivery of peptides containing transmembrane-juxtamembrane 1/2 domain. Biochem Biophys Res Commun. 2015;464:392-5 pubmed publisher
Shen W, Li H, Chen G, Chern Y, Tu P. Mutations in the ubiquitin-binding domain of OPTN/optineurin interfere with autophagy-mediated degradation of misfolded proteins by a dominant-negative mechanism. Autophagy. 2015;11:685-700 pubmed publisher
Nieznanska H, Dudek E, Zajkowski T, Szczęsna E, Kasprzak A, Nieznanski K. Prion protein impairs kinesin-driven transport. Biochem Biophys Res Commun. 2012;425:788-93 pubmed publisher
Kolev M, Tediose T, Sivasankar B, Harris C, Thome J, Morgan B, et al. Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration. Pharmacogenomics J. 2010;10:12-9 pubmed publisher

product information

Catalog Number :

05-949

Subcategory :

Epitope Tags & General Use

Product Name :

Anti-Histidine Tagged Antibody, clone HIS.H8

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

Histidine Tagged

Clone :

HIS.H8

Conjugate :

Purified

Isotype :

IgG2b

Product Description :

Anti-Histidine Tagged Antibody, clone HIS.H8

Cross Reactivity :

All

Background :

His-Tag is a series of at least 6 histidine residues used as a tag motif at the N- or C-terminus of recombinant proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. Its small size also makes it more suitable for use under denaturing conditions. His tags are most often used for affinity purification and binding assays. His tags bind to nickel or cobalt ions with high affinity, and this interaction with metal ions is the basis of many his-tag based affinity media. The His Tag epitope is widely used to detect expression of recombinant proteins in bacterial, yeast, insect,and mammalian systems.

Immunogen :

6x His synthetic peptide.

Specificity :

Recognizes tag sequence HHHHHH on either the N- or C-terminus of any recombinant fusion protein; no other amino acids surrounding the 6x-His tag sequence are required for the recognition of the 6x-His tag.

Package Size :

200 µg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.