Phosphotyrosine Antibody, clone PY20 | EMD Millipore 05-947 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Phosphotyrosine Antibody, clone PY20

catalog :

05-947

quantity :

1 mg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PY20

application :

western blot, ELISA, immunoprecipitation

citations: 36

Reference
Roy N, MacKay J, Robertson T, Hammer D, Burkhardt J. Crk adaptor proteins mediate actin-dependent T cell migration and mechanosensing induced by the integrin LFA-1. Sci Signal. 2018;11: pubmed publisher
De Mario A, Castellani A, Peggion C, Massimino M, Lim D, Hill A, et al. The prion protein constitutively controls neuronal store-operated Ca(2+) entry through Fyn kinase. Front Cell Neurosci. 2015;9:416 pubmed publisher
Freedman T, Tan Y, Skrzypczynska K, Manz B, Sjaastad F, Goodridge H, et al. LynA regulates an inflammation-sensitive signaling checkpoint in macrophages. elife. 2015;4: pubmed publisher
Illert A, Seitz A, Rummelt C, Kreutmair S, Engh R, Goodstal S, et al. Inhibition of Aurora kinase B is important for biologic activity of the dual inhibitors of BCR-ABL and Aurora kinases R763/AS703569 and PHA-739358 in BCR-ABL transformed cells. PLoS ONE. 2014;9:e112318 pubmed publisher
Hamann I, Petroll K, Grimm L, Hartwig A, Klotz L. Insulin-like modulation of Akt/FoxO signaling by copper ions is independent of insulin receptor. Arch Biochem Biophys. 2014;558:42-50 pubmed publisher
Muthusamy A, Lin C, Shanmugam S, Lindner H, ABCOUWER S, Antonetti D. Ischemia-reperfusion injury induces occludin phosphorylation/ubiquitination and retinal vascular permeability in a VEGFR-2-dependent manner. J Cereb Blood Flow Metab. 2014;34:522-31 pubmed publisher
Wang Y, Shao J, Zaro J, Shen W. Proinsulin-transferrin fusion protein as a novel long-acting insulin analog for the inhibition of hepatic glucose production. Diabetes. 2014;63:1779-88 pubmed publisher
Bloem K, Vuist I, van der Plas A, Knippels L, Garssen J, Garcia Vallejo J, et al. Ligand binding and signaling of dendritic cell immunoreceptor (DCIR) is modulated by the glycosylation of the carbohydrate recognition domain. PLoS ONE. 2013;8:e66266 pubmed publisher
Tamazato Longhi M, Cella N. Tyrosine phosphorylation plays a role in increasing maspin protein levels and its cytoplasmic accumulation. FEBS Open Bio. 2012;2:93-7 pubmed publisher
Kohler C, Dinekov M, Götz J. Active glycogen synthase kinase-3 and tau pathology-related tyrosine phosphorylation in pR5 human tau transgenic mice. Neurobiol Aging. 2013;34:1369-79 pubmed publisher
Huang Y, McNamara J. Neuroprotective effects of reactive oxygen species mediated by BDNF-independent activation of TrkB. J Neurosci. 2012;32:15521-32 pubmed publisher
Huang Y, Wu M, Pan S, Su W, Chen Y, Wu L. STAT1 activation by venous malformations mutant Tie2-R849W antagonizes VEGF-A-mediated angiogenic response partly via reduced bFGF production. Angiogenesis. 2013;16:207-22 pubmed publisher
Canonici A, Pellegrino E, Siret C, Terciolo C, Czerucka D, Bastonero S, et al. Saccharomyces boulardii improves intestinal epithelial cell restitution by inhibiting ?v?5 integrin activation state. PLoS ONE. 2012;7:e45047 pubmed publisher
Mishra J, Karanki S, Kumar N. Identification of molecular switch regulating interactions of Janus kinase 3 with cytoskeletal proteins. J Biol Chem. 2012;287:41386-91 pubmed publisher
Agrawal R, Gomez Pinilla F. 'Metabolic syndrome' in the brain: deficiency in omega-3 fatty acid exacerbates dysfunctions in insulin receptor signalling and cognition. J Physiol. 2012;590:2485-99 pubmed publisher
Leischner H, Albers C, Grundler R, Razumovskaya E, Spiekermann K, Bohlander S, et al. SRC is a signaling mediator in FLT3-ITD- but not in FLT3-TKD-positive AML. Blood. 2012;119:4026-33 pubmed publisher
Mori S, Yamada S, Kubo S, Chen J, Matsuda S, Shudou M, et al. Divalent and monovalent autoantibodies cause dysfunction of MuSK by distinct mechanisms in a rabbit model of myasthenia gravis. J Neuroimmunol. 2012;244:1-7 pubmed publisher
Ma P, Cierniewska A, Signarvic R, Cieslak M, Kong H, Sinnamon A, et al. A newly identified complex of spinophilin and the tyrosine phosphatase, SHP-1, modulates platelet activation by regulating G protein-dependent signaling. Blood. 2012;119:1935-45 pubmed publisher
Mori S, Kubo S, Akiyoshi T, Yamada S, Miyazaki T, Hotta H, et al. Antibodies against muscle-specific kinase impair both presynaptic and postsynaptic functions in a murine model of myasthenia gravis. Am J Pathol. 2012;180:798-810 pubmed publisher
Alvira D, Naughton R, Bhatt L, Tedesco S, Landry W, Cotter T. Inhibition of protein-tyrosine phosphatase 1B (PTP1B) mediates ubiquitination and degradation of Bcr-Abl protein. J Biol Chem. 2011;286:32313-23 pubmed publisher
Albers C, Illert A, Miething C, Leischner H, Thiede M, Peschel C, et al. An RNAi-based system for loss-of-function analysis identifies Raf1 as a crucial mediator of BCR-ABL-driven leukemogenesis. Blood. 2011;118:2200-10 pubmed publisher
Menon S, Kang C, Beningo K. Galectin-3 secretion and tyrosine phosphorylation is dependent on the calpain small subunit, Calpain 4. Biochem Biophys Res Commun. 2011;410:91-6 pubmed publisher
Camprecios G, Sánchez Vizcaíno E, Soley M, Ramirez I. Chronic ?-adrenergic stimulation increases ErbB receptors and cell proliferation in mouse kidney. Growth Factors. 2011;29:94-101 pubmed publisher
Park H, Cox D. Syk regulates multiple signaling pathways leading to CX3CL1 chemotaxis in macrophages. J Biol Chem. 2011;286:14762-9 pubmed publisher
Böhm S, Lamberti G, Fernández Sáiz V, Stapf C, Buchberger A. Cellular functions of Ufd2 and Ufd3 in proteasomal protein degradation depend on Cdc48 binding. Mol Cell Biol. 2011;31:1528-39 pubmed publisher
Brockmeyer C, Paster W, Pepper D, Tan C, Trudgian D, McGowan S, et al. T cell receptor (TCR)-induced tyrosine phosphorylation dynamics identifies THEMIS as a new TCR signalosome component. J Biol Chem. 2011;286:7535-47 pubmed publisher
Brantley Sieders D, Dunaway C, Rao M, Short S, Hwang Y, Gao Y, et al. Angiocrine factors modulate tumor proliferation and motility through EphA2 repression of Slit2 tumor suppressor function in endothelium. Cancer Res. 2011;71:976-87 pubmed publisher
von Bubnoff N, Gorantla S, Engh R, Oliveira T, Thöne S, Aberg E, et al. The low frequency of clinical resistance to PDGFR inhibitors in myeloid neoplasms with abnormalities of PDGFRA might be related to the limited repertoire of possible PDGFRA kinase domain mutations in vitro. Oncogene. 2011;30:933-43 pubmed publisher
RIGGS D, Yang Z, Kloss J, Loftus J. The Pyk2 FERM regulates Pyk2 complex formation and phosphorylation. Cell Signal. 2011;23:288-96 pubmed publisher
Zheng Y, Peng M, Wang Z, Asara J, Tyner A. Protein tyrosine kinase 6 directly phosphorylates AKT and promotes AKT activation in response to epidermal growth factor. Mol Cell Biol. 2010;30:4280-92 pubmed publisher
Motiwala T, Datta J, Kutay H, Roy S, Jacob S. Lyn kinase and ZAP70 are substrates of PTPROt in B-cells: Lyn inactivation by PTPROt sensitizes leukemia cells to VEGF-R inhibitor pazopanib. J Cell Biochem. 2010;110:846-56 pubmed publisher
You J, Park M, Lee Y. Dietary fish oil inhibits the early stage of recovery of atrophied soleus muscle in rats via Akt-p70s6k signaling and PGF2?. J Nutr Biochem. 2010;21:929-34 pubmed publisher
Ramírez A, Castro M, Angulo C, Ramió L, Rivera M, Torres M, et al. The presence and function of dopamine type 2 receptors in boar sperm: a possible role for dopamine in viability, capacitation, and modulation of sperm motility. Biol Reprod. 2009;80:753-61 pubmed publisher
Kurata A, Katayama R, Watanabe T, Tsuruo T, Fujita N. TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1 tyrosine phosphorylation and its downstream signaling. Cancer Sci. 2008;99:1827-34 pubmed publisher
Zuckerman S, Kao W. LC/MS identification of 12 intracellular cytoskeletal and inflammatory proteins from monocytes adherent on surface-adsorbed fibronectin-derived peptides. J Biomed Mater Res A. 2008;85:513-29 pubmed
Patel V, Rosenfeldt H, Lyons R, Servitja J, Bustelo X, Siroff M, et al. Persistent activation of Rac1 in squamous carcinomas of the head and neck: evidence for an EGFR/Vav2 signaling axis involved in cell invasion. Carcinogenesis. 2007;28:1145-52 pubmed

product information

Catalog Number :

05-947

Subcategory :

Cell Signaling

Product Name :

Anti-Phosphotyrosine Antibody, clone PY20

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Host Name :

Mouse

Antigen :

Phosphotyrosine

Clone :

PY20

Conjugate :

Purified

Isotype :

IgG2b

Product Description :

Anti-Phosphotyrosine Antibody, clone PY20

Cross Reactivity :

Vertebrates

Background :

Tyrosine phosphorylation represents a major mechanism for the activation of signal transduction pathways in response to growth factors, hormones and cytokines. There are approximately 100 tyrosine kinases that are both receptor tyrsosine kinase receptors for various growth factors and cytokines. The extent and duration of tyrosine phosphorylation are tightly regulated by the 107 protein tyrosine phosphatases. Tyrosine phosphorylation occurs on residues on either other kinases (Ser/Thr or Tyr) or on substrates. In the case of kinases, this phosphorylation often causes the enzyme to change activation states and/or it creates binding sites for SH2 domain. Mutation or misregulation of protein tyrosine kinases is a cause or consequence of many disease states. It has been estimated that 20% of oncogenes so far described are tyrosine kinases. With such clear links between aberrant protein tyrosine kinase activity and disease, it is no surprise that much of the early effort in kinase drug discovery was focused on these enzymes.

Immunogen :

Phosphotyrosine conjugated to carrier protein.

Specificity :

Tyrosine-phosphorylated proteins from all species.

Package Size :

1 mg

Uses :

ELISA;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at 2-8°C from date of recipt. bold NOTE: DO NOT FREEZE. /bold For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.