LSD1/BHC110 Antibody | EMD Millipore 05-939 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

LSD1/BHC110 Antibody

catalog :

05-939

quantity :

100 µL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

citations: 3

Published Application/Species/Sample/Dilution	Reference
ChIP-Seq; mouse; loading ...; fig 3a	Zeng X, Jedrychowski M, Chen Y, Serag S, Lavery G, Gygi S, et al. Lysine-specific demethylase 1 promotes brown adipose tissue thermogenesis via repressing glucocorticoid activation. Genes Dev. 2016;30:1822-36 pubmed publisher
	Carnesecchi J, Forcet C, Zhang L, Tribollet V, Barenton B, Boudra R, et al. ERR? induces H3K9 demethylation by LSD1 to promote cell invasion. Proc Natl Acad Sci U S A. 2017;114:3909-3914 pubmed publisher
	Brasacchio D, Okabe J, Tikellis C, Balcerczyk A, George P, Baker E, et al. Hyperglycemia induces a dynamic cooperativity of histone methylase and demethylase enzymes associated with gene-activating epigenetic marks that coexist on the lysine tail. Diabetes. 2009;58:1229-36 pubmed publisher

product information

Catalog Number :

05-939

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-LSD1/BHC110 Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O60341

Host Name :

Mouse

Antigen :

LSD1/BHC110

Conjugate :

Ascites

Product Description :

Anti-LSD1/BHC110 Antibody

Cross Reactivity :

Human;Mouse

Background :

Histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted 'Lys-4' of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

ALT Names :

BRAF35-HDAC complex protein BHC110;FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit;Flavin-containing amine oxidase domain-containing protein 2;amine oxidase (flavin containing) domain 2;lysine (K)-specific demethylase 1;lysine-specific histone demethylase 1

Immunogen :

GST fusion protein corresponding to residues 23-138 of human LSD1.

Specificity :

Recognizes LSD1, Mr 110 kDa.

Package Size :

100 µL

Uses :

Immunofluorescence;Immunohistochemistry;Immunoprecipitation;Western Blotting;Chromatin Immunoprecipitation (ChIP)

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.