This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
catalog :
05-928
quantity :
100 µL
clonality :
monoclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
A3S
reactivity :
human, mouse, brewer's yeast, thale cress
application :
western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, immunohistochemistry - paraffin section
citations: 51
Published Application/Species/Sample/DilutionReference
  • chromatin immunoprecipitation; brewer's yeast; fig 3d
House N, Polleys E, Quasem I, De la Rosa Mejia M, Joyce C, Takacsi Nagy O, et al. Distinct roles for S. cerevisiae H2A copies in recombination and repeat stability, with a role for H2A.1 threonine 126. elife. 2019;8: pubmed publisher
  • immunocytochemistry; human; loading ...; fig 6a
Redko B, Tuchinsky H, Segal T, Tobi D, Luboshits G, Ashur Fabian O, et al. Toward the development of a novel non-RGD cyclic peptide drug conjugate for treatment of human metastatic melanoma. Oncotarget. 2017;8:757-768 pubmed publisher
  • western blot; human; loading ...; fig 10c
Achuthan A, Cook A, Lee M, Saleh R, Khiew H, Chang M, et al. Granulocyte macrophage colony-stimulating factor induces CCL17 production via IRF4 to mediate inflammation. J Clin Invest. 2016;126:3453-66 pubmed publisher
  • western blot; human; 1:1000; fig 3d,4b,5e,7b
Grinshtein N, Rioseco C, Marcellus R, UEHLING D, Aman A, Lun X, et al. Small molecule epigenetic screen identifies novel EZH2 and HDAC inhibitors that target glioblastoma brain tumor-initiating cells. Oncotarget. 2016;7:59360-59376 pubmed publisher
  • western blot; mouse; loading ...; fig 7e
Shields B, Jackson J, Metcalf D, Shi W, Huang Q, Garnham A, et al. Acute myeloid leukemia requires Hhex to enable PRC2-mediated epigenetic repression of Cdkn2a. Genes Dev. 2016;30:78-91 pubmed publisher
  • chromatin immunoprecipitation; thale cress; fig 3
Husbands A, Benkovics A, Nogueira F, Lodha M, Timmermans M. The ASYMMETRIC LEAVES Complex Employs Multiple Modes of Regulation to Affect Adaxial-Abaxial Patterning and Leaf Complexity. Plant Cell. 2015;27:3321-35 pubmed publisher
  • western blot; mouse; 1:10,000; loading ...; fig 5a
Nesher E, Koman I, Gross M, Tikhonov T, Bairachnaya M, Salmon Divon M, et al. Synapsin IIb as a functional marker of submissive behavior. Sci Rep. 2015;5:10287 pubmed publisher
  • chromatin immunoprecipitation; human
Moquet Torcy G, Tolza C, Piechaczyk M, Jariel Encontre I. Transcriptional complexity and roles of Fra-1/AP-1 at the uPA/Plau locus in aggressive breast cancer. Nucleic Acids Res. 2014;42:11011-24 pubmed publisher
  • western blot; human; fig 3
Rodriguez M, Domingo E, Alonso S, Frade J, Eiros J, Crespo M, et al. The unfolded protein response and the phosphorylations of activating transcription factor 2 in the trans-activation of il23a promoter produced by ?-glucans. J Biol Chem. 2014;289:22942-57 pubmed publisher
  • western blot; human
Adeyemi R, Pintel D. The ATR signaling pathway is disabled during infection with the parvovirus minute virus of mice. J Virol. 2014;88:10189-99 pubmed publisher
  • western blot; human; fig s1
Huang Y, Leung J, Lowery M, Matsushita N, Wang Y, Shen X, et al. Modularized functions of the Fanconi anemia core complex. Cell Rep. 2014;7:1849-57 pubmed publisher
  • western blot; human; fig 6
Wu H, Balsbaugh J, Chandler H, Georgilis A, Zullow H, Shabanowitz J, et al. Mitogen-activated protein kinase signaling mediates phosphorylation of polycomb ortholog Cbx7. J Biol Chem. 2013;288:36398-408 pubmed publisher
  • western blot; human; 1:10000
Haugen M, Johansen H, Pettersen S, Solberg R, Brix K, Flatmark K, et al. Nuclear legumain activity in colorectal cancer. PLoS ONE. 2013;8:e52980 pubmed publisher
  • chromatin immunoprecipitation; mouse
McGee Lawrence M, Li X, Bledsoe K, Wu H, Hawse J, Subramaniam M, et al. Runx2 protein represses Axin2 expression in osteoblasts and is required for craniosynostosis in Axin2-deficient mice. J Biol Chem. 2013;288:5291-302 pubmed publisher
  • chromatin immunoprecipitation; human
Queen K, Shi M, Zhang F, Cvek U, Scott R. Epstein-Barr virus-induced epigenetic alterations following transient infection. Int J Cancer. 2013;132:2076-86 pubmed publisher
  • western blot; human
Ratnakumar K, Duarte L, LeRoy G, Hasson D, Smeets D, Vardabasso C, et al. ATRX-mediated chromatin association of histone variant macroH2A1 regulates ?-globin expression. Genes Dev. 2012;26:433-8 pubmed publisher
  • immunohistochemistry - paraffin section; human; fig 1
  • western blot; human; fig s23
Kapoor A, Goldberg M, Cumberland L, Ratnakumar K, Segura M, Emanuel P, et al. The histone variant macroH2A suppresses melanoma progression through regulation of CDK8. Nature. 2010;468:1105-9 pubmed publisher
Dudakovic A, Camilleri E, Paradise C, Samsonraj R, Gluscevic M, Paggi C, et al. Enhancer of zeste homolog 2 (Ezh2) controls bone formation and cell cycle progression during osteogenesis in mice. J Biol Chem. 2018;293:12894-12907 pubmed publisher
Gangula N, Maddika S. Interplay between the phosphatase PHLPP1 and E3 ligase RNF41 stimulates proper kinetochore assembly via the outer-kinetochore protein SGT1. J Biol Chem. 2017;292:13947-13958 pubmed publisher
Dudakovic A, Gluscevic M, Paradise C, Dudakovic H, Khani F, Thaler R, et al. Profiling of human epigenetic regulators using a semi-automated real-time qPCR platform validated by next generation sequencing. Gene. 2017;609:28-37 pubmed publisher
Jonsson M, Ragnum H, Julin C, Yeramian A, Clancy T, Frikstad K, et al. Hypoxia-independent gene expression signature associated with radiosensitisation of prostate cancer cell lines by histone deacetylase inhibition. Br J Cancer. 2016;115:929-939 pubmed publisher
Benoit J, Rakic P, Frick K. Prenatal stress induces spatial memory deficits and epigenetic changes in the hippocampus indicative of heterochromatin formation and reduced gene expression. Behav Brain Res. 2015;281:1-8 pubmed publisher
Donohoe D, Holley D, Collins L, Montgomery S, Whitmore A, Hillhouse A, et al. A gnotobiotic mouse model demonstrates that dietary fiber protects against colorectal tumorigenesis in a microbiota- and butyrate-dependent manner. Cancer Discov. 2014;4:1387-97 pubmed publisher
Khair L, Guikema J, Linehan E, Ucher A, Leus N, Ogilvie C, et al. ATM increases activation-induced cytidine deaminase activity at downstream S regions during class-switch recombination. J Immunol. 2014;192:4887-96 pubmed publisher
Marsman J, O Neill A, Kao B, Rhodes J, Meier M, Antony J, et al. Cohesin and CTCF differentially regulate spatiotemporal runx1 expression during zebrafish development. Biochim Biophys Acta. 2014;1839:50-61 pubmed publisher
Wang Y, Han X, Wu F, Leung J, Lowery M, Do H, et al. Structure analysis of FAAP24 reveals single-stranded DNA-binding activity and domain functions in DNA damage response. Cell Res. 2013;23:1215-28 pubmed publisher
Dudakovic A, Evans J, Li Y, Middha S, McGee Lawrence M, Van Wijnen A, et al. Histone deacetylase inhibition promotes osteoblast maturation by altering the histone H4 epigenome and reduces Akt phosphorylation. J Biol Chem. 2013;288:28783-91 pubmed publisher
Salem T, Gomard T, Court F, Moquet Torcy G, Brockly F, Forné T, et al. Chromatin loop organization of the junb locus in mouse dendritic cells. Nucleic Acids Res. 2013;41:8908-25 pubmed publisher
Singhal G, Leo E, Setty S, Pommier Y, Thimmapaya B. Adenovirus E1A oncogene induces rereplication of cellular DNA and alters DNA replication dynamics. J Virol. 2013;87:8767-78 pubmed publisher
Bravo S, Nuñez F, Cruzat F, Cafferata E, De Ferrari G, Montecino M, et al. Enhanced CRAd activity using enhancer motifs driven by a nucleosome positioning sequence. Mol Ther. 2013;21:1403-12 pubmed publisher
Niedenberger B, Chappell V, Kaye E, Renegar R, Geyer C. Nuclear localization of the actin regulatory protein Palladin in sertoli cells. Mol Reprod Dev. 2013;80:403-13 pubmed publisher
Feng L, Fong K, Wang J, Wang W, Chen J. RIF1 counteracts BRCA1-mediated end resection during DNA repair. J Biol Chem. 2013;288:11135-43 pubmed publisher
Gupta K, Thakur V, Bhaskaran N, Nawab A, Babcook M, Jackson M, et al. Green tea polyphenols induce p53-dependent and p53-independent apoptosis in prostate cancer cells through two distinct mechanisms. PLoS ONE. 2012;7:e52572 pubmed publisher
Avgousti D, Cecere G, Grishok A. The conserved PHD1-PHD2 domain of ZFP-1/AF10 is a discrete functional module essential for viability in Caenorhabditis elegans. Mol Cell Biol. 2013;33:999-1015 pubmed publisher
McGee Lawrence M, Bradley E, Dudakovic A, Carlson S, Ryan Z, Kumar R, et al. Histone deacetylase 3 is required for maintenance of bone mass during aging. Bone. 2013;52:296-307 pubmed publisher
Li H, Cai Q, Wu H, Vathipadiekal V, Dobbin Z, Li T, et al. SUZ12 promotes human epithelial ovarian cancer by suppressing apoptosis via silencing HRK. Mol Cancer Res. 2012;10:1462-72 pubmed publisher
Tai H, Serrano Pozo A, Hashimoto T, Frosch M, Spires Jones T, Hyman B. The synaptic accumulation of hyperphosphorylated tau oligomers in Alzheimer disease is associated with dysfunction of the ubiquitin-proteasome system. Am J Pathol. 2012;181:1426-35 pubmed publisher
Mehta S, Kumari S, Mendelev N, Li P. Selenium preserves mitochondrial function, stimulates mitochondrial biogenesis, and reduces infarct volume after focal cerebral ischemia. BMC Neurosci. 2012;13:79 pubmed publisher
Bhaskaran N, Shukla S, Kanwal R, Srivastava J, Gupta S. Induction of heme oxygenase-1 by chamomile protects murine macrophages against oxidative stress. Life Sci. 2012;90:1027-33 pubmed publisher
Burd C, Ward J, Crusselle Davis V, Kissling G, Phadke D, Shah R, et al. Analysis of chromatin dynamics during glucocorticoid receptor activation. Mol Cell Biol. 2012;32:1805-17 pubmed publisher
Li H, Bitler B, Vathipadiekal V, Maradeo M, Slifker M, Creasy C, et al. ALDH1A1 is a novel EZH2 target gene in epithelial ovarian cancer identified by genome-wide approaches. Cancer Prev Res (Phila). 2012;5:484-91 pubmed publisher
Friedman J, Cho W, Chu C, Keedy K, Archin N, Margolis D, et al. Epigenetic silencing of HIV-1 by the histone H3 lysine 27 methyltransferase enhancer of Zeste 2. J Virol. 2011;85:9078-89 pubmed publisher
Xu C, Cole P, Meyers D, Kormish J, Dent S, Zaret K. Chromatin "prepattern" and histone modifiers in a fate choice for liver and pancreas. Science. 2011;332:963-6 pubmed publisher
Alvarez Y, Municio C, Hugo E, Zhu J, Alonso S, Hu X, et al. Notch- and transducin-like enhancer of split (TLE)-dependent histone deacetylation explain interleukin 12 (IL-12) p70 inhibition by zymosan. J Biol Chem. 2011;286:16583-95 pubmed publisher
Cao F, Chen Y, Cierpicki T, Liu Y, Basrur V, Lei M, et al. An Ash2L/RbBP5 heterodimer stimulates the MLL1 methyltransferase activity through coordinated substrate interactions with the MLL1 SET domain. PLoS ONE. 2010;5:e14102 pubmed publisher
D Addario C, Johansson S, Candeletti S, Romualdi P, Ogren S, Terenius L, et al. Ethanol and acetaldehyde exposure induces specific epigenetic modifications in the prodynorphin gene promoter in a human neuroblastoma cell line. FASEB J. 2011;25:1069-75 pubmed publisher
Reynoird N, Schwartz B, Delvecchio M, Sadoul K, Meyers D, Mukherjee C, et al. Oncogenesis by sequestration of CBP/p300 in transcriptionally inactive hyperacetylated chromatin domains. EMBO J. 2010;29:2943-52 pubmed publisher
Rhodes J, Bentley F, Print C, Dorsett D, Misulovin Z, Dickinson E, et al. Positive regulation of c-Myc by cohesin is direct, and evolutionarily conserved. Dev Biol. 2010;344:637-49 pubmed publisher
Hoeppner L, Secreto F, Jensen E, Li X, Kahler R, Westendorf J. Runx2 and bone morphogenic protein 2 regulate the expression of an alternative Lef1 transcript during osteoblast maturation. J Cell Physiol. 2009;221:480-9 pubmed publisher
Folkvord S, Ree A, Furre T, Halvorsen T, Flatmark K. Radiosensitization by SAHA in experimental colorectal carcinoma models-in vivo effects and relevance of histone acetylation status. Int J Radiat Oncol Biol Phys. 2009;74:546-52 pubmed publisher
Huarte M, Lan F, Kim T, Vaughn M, Zaratiegui M, Martienssen R, et al. The fission yeast Jmj2 reverses histone H3 Lysine 4 trimethylation. J Biol Chem. 2007;282:21662-70 pubmed
product information
Catalog Number :
05-928
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q16695
Host Name :
Rabbit
Antigen :
Histone H3
Clone :
A3S
Conjugate :
Purified
Isotype :
IgG
Product Description :
Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
Cross Reactivity :
Human;Chicken;Rat;Mouse;Yeast ( S. cerevisiae )
Background :
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
ALT Names :
H3;Histone H3;H3 histone, family 3A
Immunogen :
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.
Specificity :
Recognizes Histone H3, Mr 17 kDa
Package Size :
100 µL
Uses :
Western Blotting;Chromatin Immunoprecipitation (ChIP)
Storage :
Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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