MEK1 Antibody, NT, clone C12T, rabbit monoclonal | EMD Millipore 05-925R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

MEK1 Antibody, NT, clone C12T, rabbit monoclonal

catalog :

05-925R

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

C12T

reactivity :

human, mouse

application :

western blot, flow cytometry

product information

Catalog Number :

05-925R

Subcategory :

Cell Signaling

Product Name :

Anti-MEK1 Antibody, NT, clone C12T, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q02750

Host Name :

Rabbit

Antigen :

MEK1

Clone :

C12T

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-MEK1 Antibody, NT, clone C12T, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

MAP Kinase/Erk Kinase (MEK), alternatively known as MKK, is a true dual-specificity kinase, in that it phosphorylates the MAP kinases on both the Thr and Tyr of the activation motif TEY. In vitro, the tyrosine phosphorylation is favored, whereas in vivo both phosphorylation events appear to occur simultaneously. This suggests that an additional factor is present in cells to facilitate the reaction. MEK1 and MEK2 are activated by phosphorylation of two serine residues (Ser218/222 in MEK1 and Ser222/226 in MEK2), which are substrates for the Raf family of kinases. Mutation of the phosphorylation sites from Ser to Asp creates a protein with constitutive kinase activity, which when expressed in cells is able to cause transformation.

ALT Names :

ERK activator kinase 1;MAP kinase kinase 1;MAPK/ERK kinase 1;MAPKK 1;mitogen-activated protein kinase kinase 1;protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Immunogen :

Recombinant peptide corresponding to amino acids surrounding the TEY motif in the activation loop of human phospho-MEK1.

Specificity :

Recognizes MEK1

Package Size :

100 µL

Uses :

Western Blotting;Flow Cytometry

Storage :

Stable for 2 years at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.