RAD21 Antibody | EMD Millipore 05-908 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

RAD21 Antibody

catalog :

05-908

quantity :

100 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunocytochemistry, immunoprecipitation

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig 1s1d	Wutz G, Ladurner R, St Hilaire B, Stocsits R, Nagasaka K, Pignard B, et al. ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL. elife. 2020;9: pubmed publisher
western blot; mouse; 1:5000; loading ...; fig 2c	Cattoglio C, Pustova I, Walther N, Ho J, Hantsche Grininger M, Inouye C, et al. Determining cellular CTCF and cohesin abundances to constrain 3D genome models. elife. 2019;8: pubmed publisher
western blot; human; 1:500; loading ...; fig 4a	Full F, van Gent M, Sparrer K, Chiang C, Zurenski M, Scherer M, et al. Centrosomal protein TRIM43 restricts herpesvirus infection by regulating nuclear lamina integrity. Nat Microbiol. 2019;4:164-176 pubmed publisher
immunoprecipitation; human; loading ...; fig s1c western blot; human; loading ...; fig s1c	Yuen K, Slaughter B, Gerton J. Condensin II is anchored by TFIIIC and H3K4me3 in the mammalian genome and supports the expression of active dense gene clusters. Sci Adv. 2017;3:e1700191 pubmed publisher
western blot; human; 1:100; loading ...; fig s1c	Haarhuis J, van der Weide R, Blomen V, Yáñez Cuna J, Amendola M, van Ruiten M, et al. The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension. Cell. 2017;169:693-707.e14 pubmed publisher
immunocytochemistry; mouse; loading ...; fig 1b western blot; mouse; loading ...; fig 1a	Busslinger G, Stocsits R, van der Lelij P, Axelsson E, Tedeschi A, Galjart N, et al. Cohesin is positioned in mammalian genomes by transcription, CTCF and Wapl. Nature. 2017;544:503-507 pubmed publisher
western blot; mouse; fig 5	Ladurner R, Kreidl E, Ivanov M, Ekker H, Idarraga Amado M, Busslinger G, et al. Sororin actively maintains sister chromatidÂ cohesion. EMBO J. 2016;35:635-53 pubmed publisher
immunocytochemistry; human; fig 6	Wike C, Graves H, Hawkins R, Gibson M, Ferdinand M, Zhang T, et al. Aurora-A mediated histone H3 phosphorylation of threonine 118 controls condensin I and cohesin occupancy in mitosis. elife. 2016;5:e11402 pubmed publisher
western blot; human; fig 3	Gelot C, Guirouilh Barbat J, Le Guen T, Dardillac E, Chailleux C, Canitrot Y, et al. The Cohesin Complex Prevents the End Joining of Distant DNA Double-Strand Ends. Mol Cell. 2016;61:15-26 pubmed publisher
western blot; human	van der Lelij P, Stocsits R, Ladurner R, Petzold G, Kreidl E, Koch B, et al. SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs. EMBO J. 2014;33:2643-58 pubmed publisher
western blot; human	Gosalia N, Neems D, Kerschner J, Kosak S, Harris A. Architectural proteins CTCF and cohesin have distinct roles in modulating the higher order structure and expression of the CFTR locus. Nucleic Acids Res. 2014;42:9612-22 pubmed publisher
	Li Y, Haarhuis J, Cacciatore Á, Oldenkamp R, van Ruiten M, Willems L, et al. The structural basis for cohesin-CTCF-anchored loops. Nature. 2020;: pubmed publisher
	Eykelenboom J, Gierlinski M, Yue Z, Hegarat N, Pollard H, Fukagawa T, et al. Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis. J Cell Biol. 2019;218:1531-1552 pubmed publisher
	Shimamoto Y, Tamura S, Masumoto H, Maeshima K. Nucleosome-nucleosome interactions via histone tails and linker DNA regulate nuclear rigidity. Mol Biol Cell. 2017;28:1580-1589 pubmed publisher
	Davidson I, Goetz D, Zaczek M, Molodtsov M, Huis In t Veld P, Weissmann F, et al. Rapid movement and transcriptional re-localization of human cohesin on DNA. EMBO J. 2016;35:2671-2685 pubmed
	Majumder P, Boss J. Cohesin regulates MHC class II genes through interactions with MHC class II insulators. J Immunol. 2011;187:4236-44 pubmed publisher

product information

Catalog Number :

05-908

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-RAD21 Antibody

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

O60216

Host Name :

Mouse

Antigen :

RAD21

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-RAD21 Antibody

Cross Reactivity :

Human;Mouse

Background :

The protein encoded by the Rad21 gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. RAD21 also plays a role in apoptosis, via its cleavage by caspase 3 or caspase 7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway. Recent work has shown that the RAD21 has a role in the invasion and metastasis of cancer cells

Immunogen :

GST fusion protein corresponding to residues 240-631 of human Rad21

Specificity :

Rad21

Package Size :

100 µg

Uses :

Immunoprecipitation;Western Blotting;Immunocytochemistry

Storage :

Stable for 1 year at 2-8°C from date of receipt