phospho-STAT5A/B (Tyr694/699) Antibody, clone 94-10C-9-10C-2, rabbit monoclonal | EMD Millipore 05-886R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-STAT5A/B (Tyr694/699) Antibody, clone 94-10C-9-10C-2, rabbit monoclonal

catalog :

05-886R

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

94-10C-9-10C-2

reactivity :

human, mouse

application :

western blot

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; loading ...; fig s5a	Bigenzahn J, Collu G, Kartnig F, Pieraks M, Vladimer G, Heinz L, et al. LZTR1 is a regulator of RAS ubiquitination and signaling. Science. 2018;362:1171-1177 pubmed publisher
western blot; human; loading ...; fig 3b	Bigenzahn J, Fauster A, Rebsamen M, Kandasamy R, Scorzoni S, Vladimer G, et al. An Inducible Retroviral Expression System for Tandem Affinity Purification Mass-Spectrometry-Based Proteomics Identifies Mixed Lineage Kinase Domain-like Protein (MLKL) as an Heat Shock Protein 90 (HSP90) Client. Mol Cell Proteomics. 2016;15:1139-50 pubmed

product information

Catalog Number :

05-886R

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 94-10C-9-10C-2, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P42229

Host Name :

Rabbit

Antigen :

phospho-STAT5A/B (Tyr694/699)

Clone :

94-10C-9-10C-2

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone 94-10C-9-10C-2, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

STAT (Signal Transducers and Activators of Transcription) are a family of conserved transcription factors that transduce high-fidelity signals for the cytokine family of ligands and receptors as the principle substrates of JAK kinases (1,2). In resting cells, the STATs exist in an inactive state in the cytoplasm until activated. Activators include cytokines, growth factors, and some peptides. These activate JAK kinases, which when activated, bind to the STAT SH2 (Src homology domain-2) domain resulting in phosphorylation of STAT (1). Upon activation, STAT1, -3, -4, -5A, and -5B all form homodimers. STAT1 and STAT2, as well as STAT1 and STAT3 form heterodimers, and several STATs form tetramers, depending on the variables of the activating ligand (1). After dimerization, the STATs accumulate in the nucleus by way of nuclear transport (1). STAT5A and STAT5B appear to be most important to the function of growth hormone and prolactin and play critical roles in milk production (1,2,3). The phosphorylation of Tyrosine 694/699 is a hallmark of activated STAT5.

ALT Names :

signal transducer and activator of transcription 5A/B

Immunogen :

KLH-conjugated linear peptide corresponding to phosphorylated human STAT5A/B at and around Tyr694/Tyr699.

Specificity :

Recognizes phosphorylated STAT5A/B atTyr694/Tyr699.

Package Size :

100 µL

Uses :

Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.