PLK1 Antibody, clone 35-206 | EMD Millipore 05-844 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

PLK1 Antibody, clone 35-206

catalog :

05-844

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

35-206

The same clone is also sold as:

MilliporeSigma: P5998
Abcam: ab17056
Invitrogen: 37-7000

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, western blot knockout validation

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; fig S4	Helmke C, Raab M, Rodel F, Matthess Y, Oellerich T, Mandal R, et al. Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8. Cell Res. 2016;26:914-34 pubmed publisher
immunohistochemistry - paraffin section; human; loading ...; fig 6f	Chaisaingmongkol J, Budhu A, Dang H, Rabibhadana S, Pupacdi B, Kwon S, et al. Common Molecular Subtypes Among Asian Hepatocellular Carcinoma and Cholangiocarcinoma. Cancer Cell. 2017;32:57-70.e3 pubmed publisher
western blot; human; fig 3	Kim M, Caplen N, Chakka S, Hernandez L, House C, Pongas G, et al. Identification of therapeutic targets applicable to clinical strategies in ovarian cancer. BMC Cancer. 2016;16:678 pubmed publisher
immunoprecipitation; human; fig 4 western blot; human; fig 4	Moudry P, Watanabe K, Wolanin K, Bartkova J, Wassing I, Watanabe S, et al. TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. J Cell Biol. 2016;212:281-8 pubmed publisher
immunoprecipitation; human; fig 3	Zou J, Zhang D, Qin G, Chen X, Wang H, Zhang D. BRCA1 and FancJ cooperatively promote interstrand crosslinker induced centrosome amplification through the activation of polo-like kinase 1. Cell Cycle. 2014;13:3685-97 pubmed publisher
immunoprecipitation; human immunocytochemistry; human western blot; human	Matthess Y, Raab M, Knecht R, Becker S, Strebhardt K. Sequential Cdk1 and Plk1 phosphorylation of caspase-8 triggers apoptotic cell death during mitosis. Mol Oncol. 2014;8:596-608 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1200 western blot; human; 1:1000	Linton A, Cheng Y, Griggs K, Schedlich L, Kirschner M, Gattani S, et al. An RNAi-based screen reveals PLK1, CDK1 and NDC80 as potential therapeutic targets in malignant pleural mesothelioma. Br J Cancer. 2014;110:510-9 pubmed publisher
immunocytochemistry; mouse; 1 ug/mL western blot; mouse; 1 ug/mL	Fingas C, Mertens J, Razumilava N, Sydor S, Bronk S, Christensen J, et al. Polo-like kinase 2 is a mediator of hedgehog survival signaling in cholangiocarcinoma. Hepatology. 2013;58:1362-74 pubmed publisher
	Feldman M, Levy D. Peptide inhibition of the SETD6 methyltransferase catalytic activity. Oncotarget. 2018;9:4875-4885 pubmed publisher
	Schlatterer S, Suh H, Conejero Goldberg C, Chen S, Acker C, Lee S, et al. Neuronal c-Abl activation leads to induction of cell cycle and interferon signaling pathways. J Neuroinflammation. 2012;9:208 pubmed publisher
	Down C, Millour J, Lam E, Watson R. Binding of FoxM1 to G2/M gene promoters is dependent upon B-Myb. Biochim Biophys Acta. 2012;1819:855-62 pubmed publisher

product information

Catalog Number :

05-844

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-PLK1 Antibody, clone 35-206

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P53350

Host Name :

Mouse

Antigen :

PLK1

Clone :

clone 35-206

Conjugate :

Purified

Isotype :

IgG2b

Product Description :

Anti-PLK1 Antibody, clone 35-206

Cross Reactivity :

Human;Mouse;Rat

Background :

Polo-like Kinase 1 (PLK-1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK-1 protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.

ALT Names :

Polo-like kinase 1;Serine/threonine-protein kinase 13;cell cycle regulated protein kinase;polo (Drosophia)-like kinase;polo like kinase;polo like kinase;polo-like kinase (Drosophila);polo-like kinase 1 (Drosophila)

Immunogen :

6His-tagged full length fusion protein corresponding to human polo-like kinase 1 (PLK1). Also called serine-threonine protein kinase PLK.

Specificity :

PLK1

Package Size :

200 μg

Uses :

Western Blotting;Immunoprecipitation;Immunocytochemistry;Immunohistochemistry

Storage :

Stable for 1 year at from date of receipt.