This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
α-Tubulin Antibody, clone DM1A
catalog :
05-829
quantity :
200 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DM1A

The same clone is also sold as:
reactivity :
human, mouse, rat, bovine, zebrafish , roundworm
application :
western blot, immunocytochemistry, flow cytometry
citations: 137
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 2d
Miles R, Kerridge C, Hilditch L, Monit C, Jacques D, Towers G. MxB sensitivity of HIV-1 is determined by a highly variable and dynamic capsid surface. elife. 2020;9: pubmed publisher
  • western blot; mouse; loading ...; fig 3d, 3e
Satoh A, Han S, Araki M, Nakagawa Y, Ohno H, Mizunoe Y, et al. CREBH Improves Diet-Induced Obesity, Insulin Resistance, and Metabolic Disturbances by FGF21-Dependent and FGF21-Independent Mechanisms. iScience. 2020;23:100930 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig e5d
Eckert M, Coscia F, Chryplewicz A, Chang J, Hernandez K, Pan S, et al. Proteomics reveals NNMT as a master metabolic regulator of cancer-associated fibroblasts. Nature. 2019;: pubmed publisher
  • western blot; human; loading ...; fig 5d
Komori H, Goto Y, Kurayoshi K, Ozono E, Iwanaga R, Bradford A, et al. Differential requirement for dimerization partner DP between E2F-dependent activation of tumor suppressor and growth-related genes. Sci Rep. 2018;8:8438 pubmed publisher
  • western blot; human; 1:500; fig 5
Vogan J, Zhang X, Youmans D, Regalado S, Johnson J, Hockemeyer D, et al. Minimized human telomerase maintains telomeres and resolves endogenous roles of H/ACA proteins, TCAB1, and Cajal bodies. elife. 2016;5: pubmed publisher
  • western blot; human; 1:5000; fig 2
Meinhardt G, Saleh L, Otti G, Haider S, Velicky P, Fiala C, et al. Wingless ligand 5a is a critical regulator of placental growth and survival. Sci Rep. 2016;6:28127 pubmed publisher
  • western blot; mouse; loading ...; fig 2d
Nakagawa Y, Oikawa F, Mizuno S, Ohno H, Yagishita Y, Satoh A, et al. Hyperlipidemia and hepatitis in liver-specific CREB3L3 knockout mice generated using a one-step CRISPR/Cas9 system. Sci Rep. 2016;6:27857 pubmed publisher
  • western blot; human; fig 1
Lee J, Kuo C, Tsai S, Cheng S, Chen S, Chan H, et al. Inhibition of HDAC3- and HDAC6-Promoted Survivin Expression Plays an Important Role in SAHA-Induced Autophagy and Viability Reduction in Breast Cancer Cells. Front Pharmacol. 2016;7:81 pubmed publisher
  • western blot; human; fig 5
Vincent A, Berthel E, Dacheux E, Magnard C, Venezia N. BRCA1 affects protein phosphatase 6 signalling through its interaction with ANKRD28. Biochem J. 2016;473:949-60 pubmed publisher
  • western blot; human; fig s2
Bleckmann A, Conradi L, Menck K, Schmick N, Schubert A, Rietkötter E, et al. β-catenin-independent WNT signaling and Ki67 in contrast to the estrogen receptor status are prognostic and associated with poor prognosis in breast cancer liver metastases. Clin Exp Metastasis. 2016;33:309-23 pubmed publisher
  • western blot; zebrafish ; 1:1000; fig s1
Jayachandran P, Olmo V, Sanchez S, McFarland R, Vital E, Werner J, et al. Microtubule-associated protein 1b is required for shaping the neural tube. Neural Dev. 2016;11:1 pubmed publisher
  • western blot; human; 1:5000; fig 5
Soo Lee N, Jin Chung H, Kim H, Yun Lee S, Ji J, Seo Y, et al. TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage. Nat Commun. 2016;7:10463 pubmed publisher
  • western blot; mouse; fig 1
Kitamura A, Nakayama Y, Shibasaki A, Taki A, Yuno S, Takeda K, et al. Interaction of RNA with a C-terminal fragment of the amyotrophic lateral sclerosis-associated TDP43 reduces cytotoxicity. Sci Rep. 2016;6:19230 pubmed publisher
  • western blot; roundworm ; 1:5000; fig 2
Zhang L, Ward J, Cheng Z, Dernburg A. The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans. Development. 2015;142:4374-84 pubmed publisher
  • western blot; human; fig 8d
Lin K, Kao S, Lai C, Chen C, Wu C, Hsu H, et al. Tumor Suppressor Lzap Suppresses Wnt/β-Catenin Signaling to Promote Zebrafish Embryonic Ventral Cell Fates via the Suppression of Inhibitory Phosphorylation of Glycogen Synthase Kinase 3. J Biol Chem. 2015;290:29808-19 pubmed publisher
  • western blot; human; 1:1000; fig 4
Nakakido M, Deng Z, Suzuki T, Dohmae N, Nakamura Y, Hamamoto R. PRMT6 increases cytoplasmic localization of p21CDKN1A in cancer cells through arginine methylation and makes more resistant to cytotoxic agents. Oncotarget. 2015;6:30957-67 pubmed publisher
  • western blot; mouse; 1:4000; fig 1
Wong P, Feng Y, Wang J, Shi R, Jiang X. Regulation of autophagy by coordinated action of mTORC1 and protein phosphatase 2A. Nat Commun. 2015;6:8048 pubmed publisher
  • western blot; rat; 1:1000
Hirunsai M, Srikuea R, Yimlamai T. Heat stress promotes extracellular matrix remodelling via TGF-β1 and MMP-2/TIMP-2 modulation in tenotomised soleus and plantaris muscles. Int J Hyperthermia. 2015;31:336-48 pubmed publisher
  • western blot; human; fig s3
Menck K, Scharf C, Bleckmann A, Dyck L, Rost U, Wenzel D, et al. Tumor-derived microvesicles mediate human breast cancer invasion through differentially glycosylated EMMPRIN. J Mol Cell Biol. 2015;7:143-53 pubmed publisher
  • western blot; mouse; fig 2e
Kong S, Thiruppathi M, Qiu Q, Lin Z, Dong H, Chini E, et al. DBC1 is a suppressor of B cell activation by negatively regulating alternative NF-κB transcriptional activity. J Immunol. 2014;193:5515-24 pubmed publisher
  • western blot; human; 1:1000
Deng Z, Matsuda K, Tanikawa C, Lin J, Furukawa Y, Hamamoto R, et al. Late Cornified Envelope Group I, a novel target of p53, regulates PRMT5 activity. Neoplasia. 2014;16:656-64 pubmed publisher
  • immunocytochemistry; mouse; 1:200
Strakova J, Dean J, Sharpe K, Meyers T, Odom G, Townsend D. Dystrobrevin increases dystrophin's binding to the dystrophin-glycoprotein complex and provides protection during cardiac stress. J Mol Cell Cardiol. 2014;76:106-15 pubmed publisher
  • western blot; human
  • western blot; mouse
Yang B, Wagner J, Damaschke N, Yao T, Wuerzberger Davis S, Lee M, et al. A novel pathway links oxidative stress to loss of insulin growth factor-2 (IGF2) imprinting through NF-?B activation. PLoS ONE. 2014;9:e88052 pubmed publisher
  • western blot; human
Almenar Queralt A, Kim S, Benner C, Herrera C, Kang D, Garcia Bassets I, et al. Presenilins regulate neurotrypsin gene expression and neurotrypsin-dependent agrin cleavage via cyclic AMP response element-binding protein (CREB) modulation. J Biol Chem. 2013;288:35222-36 pubmed publisher
  • western blot; human; fig 7
Barbeau D, La K, Kim D, Kerpedjieva S, Shurin G, Tamama K. Early growth response-2 signaling mediates immunomodulatory effects of human multipotential stromal cells. Stem Cells Dev. 2014;23:155-66 pubmed publisher
  • western blot; mouse
Ma T, Galimberti F, Erkmen C, Memoli V, Chinyengetere F, SEMPERE L, et al. Comparing histone deacetylase inhibitor responses in genetically engineered mouse lung cancer models and a window of opportunity trial in patients with lung cancer. Mol Cancer Ther. 2013;12:1545-55 pubmed publisher
  • western blot; human
Ferro M, Giuberti G, Zappavigna S, Perdonà S, Facchini G, Sperlongano P, et al. Chondroitin sulphate enhances the antitumor activity of gemcitabine and mitomycin-C in bladder cancer cells with different mechanisms. Oncol Rep. 2012;27:409-15 pubmed publisher
  • western blot; human
Hsu F, Yang M, Lin E, Tseng C, Lin H. The significance of Her2 on androgen receptor protein stability in the transition of androgen requirement in prostate cancer cells. Am J Physiol Endocrinol Metab. 2011;300:E902-8 pubmed publisher
Yu B, Ma J, Li J, Wang D, Wang Z, Wang S. Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics. Nat Commun. 2020;11:2549 pubmed publisher
Lu Y, Stuart J, Talbot Cooper C, Agrawal Singh S, Huntly B, Smid A, et al. Histone deacetylase 4 promotes type I interferon signaling, restricts DNA viruses, and is degraded via vaccinia virus protein C6. Proc Natl Acad Sci U S A. 2019;116:11997-12006 pubmed publisher
Pallett M, Ren H, Zhang R, Scutts S, Gonzalez L, Zhu Z, et al. Vaccinia Virus BBK E3 Ligase Adaptor A55 Targets Importin-Dependent NF-κB Activation and Inhibits CD8+ T-Cell Memory. J Virol. 2019;93: pubmed publisher
Sulli G, Rommel A, Wang X, Kolar M, Puca F, Saghatelian A, et al. Pharmacological activation of REV-ERBs is lethal in cancer and oncogene-induced senescence. Nature. 2018;553:351-355 pubmed publisher
Lee Y, Lim J, Jeoun U, Min S, Lee E, Kwon S, et al. Lactate-mediated mitoribosomal defects impair mitochondrial oxidative phosphorylation and promote hepatoma cell invasiveness. J Biol Chem. 2017;292:20208-20217 pubmed publisher
Chen Y, Chen Y, Chang Y, Wu C, Ko Y, Tsao S, et al. Epstein-Barr Virus Rta-Mediated Accumulation of DNA Methylation Interferes with CTCF Binding in both Host and Viral Genomes. J Virol. 2017;91: pubmed publisher
Na T, Kim G, Oh H, Lee M, Han Y, Kim K, et al. The trisaccharide raffinose modulates epidermal differentiation through activation of liver X receptor. Sci Rep. 2017;7:43823 pubmed publisher
Huang G, Ge G, Izzi V, Greenspan D. α3 Chains of type V collagen regulate breast tumour growth via glypican-1. Nat Commun. 2017;8:14351 pubmed publisher
Hentati Kallel M, Le Jan S, Bernard P, Antonicelli F, Trussardi Regnier A. Histone deacetylases meet microRNA-associated MMP-9 expression regulation in glucocorticoid-sensitive and -resistant cell lines. Int J Oncol. 2017;50:717-726 pubmed publisher
Wan L, Maisonneuve P, Szilard R, Lambert J, Ng T, Manczyk N, et al. Proteomic analysis of the human KEOPS complex identifies C14ORF142 as a core subunit homologous to yeast Gon7. Nucleic Acids Res. 2017;45:805-817 pubmed publisher
Kim G, Lee S, Cho H, Kim M, Shin E, Oh J. Hepatitis C Virus Core Protein Promotes miR-122 Destabilization by Inhibiting GLD-2. PLoS Pathog. 2016;12:e1005714 pubmed publisher
Weaver E, Zamora F, Puplampu Dove Y, Kiessu E, Hearne J, Martin Caraballo M. Regulation of T-type calcium channel expression by sodium butyrate in prostate cancer cells. Eur J Pharmacol. 2015;749:20-31 pubmed publisher
Rwigemera A, Mamelona J, Martin L. Comparative effects between fucoxanthinol and its precursor fucoxanthin on viability and apoptosis of breast cancer cell lines MCF-7 and MDA-MB-231. Anticancer Res. 2015;35:207-19 pubmed
Huynh F, Jones F. MicroRNA-7 inhibits multiple oncogenic pathways to suppress HER2Δ16 mediated breast tumorigenesis and reverse trastuzumab resistance. PLoS ONE. 2014;9:e114419 pubmed publisher
Stroebel D, Carvalho S, Grand T, Zhu S, Paoletti P. Controlling NMDA receptor subunit composition using ectopic retention signals. J Neurosci. 2014;34:16630-6 pubmed publisher
Kacsinta A, Rubenstein C, Sroka I, Pawar S, Gard J, Nagle R, et al. Intracellular modifiers of integrin alpha 6p production in aggressive prostate and breast cancer cell lines. Biochem Biophys Res Commun. 2014;454:335-40 pubmed publisher
Mardilovich K, Gabrielsen M, McGarry L, Orange C, Patel R, Shanks E, et al. Elevated LIM kinase 1 in nonmetastatic prostate cancer reflects its role in facilitating androgen receptor nuclear translocation. Mol Cancer Ther. 2015;14:246-58 pubmed publisher
Jansen S, Holman R, Hedemann I, Frankes E, Elzinga C, Timens W, et al. Prostaglandin E2 promotes MYCN non-amplified neuroblastoma cell survival via β-catenin stabilization. J Cell Mol Med. 2015;19:210-26 pubmed publisher
Chan M, Gravel M, Bramoullé A, Bridon G, Avizonis D, Shore G, et al. Synergy between the NAMPT inhibitor GMX1777(8) and pemetrexed in non-small cell lung cancer cells is mediated by PARP activation and enhanced NAD consumption. Cancer Res. 2014;74:5948-54 pubmed publisher
Ishii H, Saitoh M, Sakamoto K, Kondo T, Katoh R, Tanaka S, et al. Epithelial splicing regulatory proteins 1 (ESRP1) and 2 (ESRP2) suppress cancer cell motility via different mechanisms. J Biol Chem. 2014;289:27386-99 pubmed publisher
Díaz N, Morera L, Verra D, Contin M, Guido M. Early appearance of nonvisual and circadian markers in the developing inner retinal cells of chicken. Biomed Res Int. 2014;2014:646847 pubmed publisher
Lin L, Yang S, Chu J, Wang L, Ning L, Zhang T, et al. Region-specific expression of tau, amyloid-? protein precursor, and synaptic proteins at physiological condition or under endoplasmic reticulum stress in rats. J Alzheimers Dis. 2014;41:1149-63 pubmed publisher
Rwigemera A, Mamelona J, Martin L. Inhibitory effects of fucoxanthinol on the viability of human breast cancer cell lines MCF-7 and MDA-MB-231 are correlated with modulation of the NF-kappaB pathway. Cell Biol Toxicol. 2014;30:157-67 pubmed publisher
Sullivan S, Dillon G, Sullivan J, Ho A. Mint proteins are required for synaptic activity-dependent amyloid precursor protein (APP) trafficking and amyloid ? generation. J Biol Chem. 2014;289:15374-83 pubmed publisher
Khiati S, Dalla Rosa I, Sourbier C, Ma X, Rao V, Neckers L, et al. Mitochondrial topoisomerase I (top1mt) is a novel limiting factor of doxorubicin cardiotoxicity. Clin Cancer Res. 2014;20:4873-81 pubmed publisher
Lysko D, Putt M, Golden J. SDF1 reduces interneuron leading process branching through dual regulation of actin and microtubules. J Neurosci. 2014;34:4941-62 pubmed publisher
Almenar Queralt A, Falzone T, Shen Z, Lillo C, Killian R, Arreola A, et al. UV irradiation accelerates amyloid precursor protein (APP) processing and disrupts APP axonal transport. J Neurosci. 2014;34:3320-39 pubmed publisher
Maida Y, Yasukawa M, Okamoto N, Ohka S, Kinoshita K, Totoki Y, et al. Involvement of telomerase reverse transcriptase in heterochromatin maintenance. Mol Cell Biol. 2014;34:1576-93 pubmed publisher
Sokolowski J, Gamage K, Heffron D, LeBlanc A, Deppmann C, Mandell J. Caspase-mediated cleavage of actin and tubulin is a common feature and sensitive marker of axonal degeneration in neural development and injury. Acta Neuropathol Commun. 2014;2:16 pubmed publisher
Ting C, Huang H, Huang T, Wu C, Chen J. The mechanisms by which pardaxin, a natural cationic antimicrobial peptide, targets the endoplasmic reticulum and induces c-FOS. Biomaterials. 2014;35:3627-40 pubmed publisher
Wong Y, Holzbaur E. The regulation of autophagosome dynamics by huntingtin and HAP1 is disrupted by expression of mutant huntingtin, leading to defective cargo degradation. J Neurosci. 2014;34:1293-305 pubmed publisher
Bazzini C, Rossetti V, Civello D, Sassone F, Vezzoli V, Persani L, et al. Short- and long- term effects of cigarette smoke exposure on glutathione homeostasis in human bronchial epithelial cells. Cell Physiol Biochem. 2013;32:129-45 pubmed publisher
Jiang Q, Wang L, Guan Y, Xu H, Niu Y, Han L, et al. Golgin-84-associated Golgi fragmentation triggers tau hyperphosphorylation by activation of cyclin-dependent kinase-5 and extracellular signal-regulated kinase. Neurobiol Aging. 2014;35:1352-63 pubmed publisher
Chang K, Chang W, Chang Y, Hung L, Lai C, Yeh Y, et al. Ran GTPase-activating protein 1 is a therapeutic target in diffuse large B-cell lymphoma. PLoS ONE. 2013;8:e79863 pubmed publisher
Rossi G, Conconi D, Panzeri E, Paoletta L, Piccoli E, Ferretti M, et al. Mutations in MAPT give rise to aneuploidy in animal models of tauopathy. Neurogenetics. 2014;15:31-40 pubmed publisher
Grivas P, Day K, Karatsinides A, Paul A, Shakir N, Owainati I, et al. Evaluation of the antitumor activity of dacomitinib in models of human bladder cancer. Mol Med. 2013;19:367-76 pubmed publisher
Hoshi T, Tezuka T, Yokoyama K, Iemura S, Natsume T, Yamanashi Y. Mesdc2 plays a key role in cell-surface expression of Lrp4 and postsynaptic specialization in myotubes. FEBS Lett. 2013;587:3749-54 pubmed publisher
Wang X, Wang L, Tang H, Shan W, Wang X, Liu D, et al. Acetyl-L-carnitine rescues scopolamine-induced memory deficits by restoring insulin-like growth factor II via decreasing p53 oxidation. Neuropharmacology. 2014;76 Pt A:80-7 pubmed publisher
Barrio L, Sáez de Guinoa J, Carrasco Y. TLR4 signaling shapes B cell dynamics via MyD88-dependent pathways and Rac GTPases. J Immunol. 2013;191:3867-75 pubmed publisher
Beharry C, Alaniz M, Alonso A. Expression of Alzheimer-like pathological human tau induces a behavioral motor and olfactory learning deficit in Drosophila melanogaster. J Alzheimers Dis. 2013;37:539-50 pubmed publisher
Funahashi Y, Namba T, Fujisue S, Itoh N, Nakamuta S, Kato K, et al. ERK2-mediated phosphorylation of Par3 regulates neuronal polarization. J Neurosci. 2013;33:13270-85 pubmed publisher
Kozono S, Ohuchida K, Ohtsuka T, Cui L, Eguchi D, Fujiwara K, et al. S100A4 mRNA expression level is a predictor of radioresistance of pancreatic cancer cells. Oncol Rep. 2013;30:1601-8 pubmed publisher
Silberberg Y, Kawamura R, Ryu S, Fukazawa K, Ishihara K, Nakamura C. Detection of microtubules in vivo using antibody-immobilized nanoneedles. J Biosci Bioeng. 2014;117:107-12 pubmed publisher
Buttler K, Becker J, Pukrop T, Wilting J. Maldevelopment of dermal lymphatics in Wnt5a-knockout-mice. Dev Biol. 2013;381:365-76 pubmed publisher
Geißler V, Altmeyer S, Stein B, Uhlmann Schiffler H, Stahl H. The RNA helicase Ddx5/p68 binds to hUpf3 and enhances NMD of Ddx17/p72 and Smg5 mRNA. Nucleic Acids Res. 2013;41:7875-88 pubmed publisher
Vilagran I, Castillo J, Bonet S, Sancho S, Yeste M, Estanyol J, et al. Acrosin-binding protein (ACRBP) and triosephosphate isomerase (TPI) are good markers to predict boar sperm freezing capacity. Theriogenology. 2013;80:443-50 pubmed publisher
Fradet Turcotte A, Canny M, Escribano Diaz C, Orthwein A, Leung C, Huang H, et al. 53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark. Nature. 2013;499:50-4 pubmed publisher
Huang Z, Murphy S. Increased Intragenic IGF2 Methylation is Associated with Repression of Insulator Activity and Elevated Expression in Serous Ovarian Carcinoma. Front Oncol. 2013;3:131 pubmed publisher
Motizuki M, Isogaya K, Miyake K, Ikushima H, Kubota T, Miyazono K, et al. Oligodendrocyte transcription factor 1 (Olig1) is a Smad cofactor involved in cell motility induced by transforming growth factor-?. J Biol Chem. 2013;288:18911-22 pubmed publisher
Brochier C, Dennis G, Rivieccio M, McLaughlin K, Coppola G, Ratan R, et al. Specific acetylation of p53 by HDAC inhibition prevents DNA damage-induced apoptosis in neurons. J Neurosci. 2013;33:8621-32 pubmed publisher
Gillis J, Selth L, Centenera M, Townley S, Sun S, Plymate S, et al. Constitutively-active androgen receptor variants function independently of the HSP90 chaperone but do not confer resistance to HSP90 inhibitors. Oncotarget. 2013;4:691-704 pubmed
Park S, Riley P, Frisch S. Regulation of anoikis by deleted in breast cancer-1 (DBC1) through NF-κB. Apoptosis. 2013;18:949-62 pubmed publisher
Maor Nof M, Homma N, Raanan C, Nof A, Hirokawa N, Yaron A. Axonal pruning is actively regulated by the microtubule-destabilizing protein kinesin superfamily protein 2A. Cell Rep. 2013;3:971-7 pubmed publisher
Márquez Navarro A, Pérez Reyes A, Zepeda Rodriguez A, Reynoso Ducoing O, Hernández Campos A, Hernández Luis F, et al. RCB20, an experimental benzimidazole derivative, affects tubulin expression and induces gross anatomical changes in Taenia crassiceps cysticerci. Parasitol Res. 2013;112:2215-26 pubmed publisher
Kuszczyk M, Sanchez S, Pankiewicz J, Kim J, Duszczyk M, Guridi M, et al. Blocking the interaction between apolipoprotein E and A? reduces intraneuronal accumulation of A? and inhibits synaptic degeneration. Am J Pathol. 2013;182:1750-68 pubmed publisher
Tang J, Michaelis K, Nozik Grayck E, Seedorf G, Hartman Filson M, Abman S, et al. The NF-?B inhibitory proteins I?B? and I?B? mediate disparate responses to inflammation in fetal pulmonary endothelial cells. J Immunol. 2013;190:2913-23 pubmed publisher
Sun C, Nirmalananda S, Jenkins C, Debnath S, Balambika R, Fata J, et al. First ayurvedic approach towards green drugs: anti cervical cancer-cell properties of Clerodendrum viscosum root extract. Anticancer Agents Med Chem. 2013;13:1469-76 pubmed
Kozono S, Ohuchida K, Eguchi D, Ikenaga N, Fujiwara K, Cui L, et al. Pirfenidone inhibits pancreatic cancer desmoplasia by regulating stellate cells. Cancer Res. 2013;73:2345-56 pubmed publisher
Bubenshchikova E, Ichimura K, Fukuyo Y, Powell R, Hsu C, Morrical S, et al. Wtip and Vangl2 are required for mitotic spindle orientation and cloaca morphogenesis. Biol Open. 2012;1:588-96 pubmed publisher
Dalvai M, Bellucci L, Fleury L, Lavigne A, Moutahir F, Bystricky K. H2A.Z-dependent crosstalk between enhancer and promoter regulates cyclin D1 expression. Oncogene. 2013;32:4243-51 pubmed publisher
Buchsbaum S, Bercovich B, Ziv T, Ciechanover A. Modification of the inflammatory mediator LRRFIP2 by the ubiquitin-like protein FAT10 inhibits its activity during cellular response to LPS. Biochem Biophys Res Commun. 2012;428:11-6 pubmed publisher
Manni M, De Siena G, Saba A, Marchini M, Landucci E, Gerace E, et al. Pharmacological effects of 3-iodothyronamine (T1AM) in mice include facilitation of memory acquisition and retention and reduction of pain threshold. Br J Pharmacol. 2013;168:354-62 pubmed publisher
Eguchi D, Ikenaga N, Ohuchida K, Kozono S, Cui L, Fujiwara K, et al. Hypoxia enhances the interaction between pancreatic stellate cells and cancer cells via increased secretion of connective tissue growth factor. J Surg Res. 2013;181:225-33 pubmed publisher
Chaufty J, Sullivan S, Ho A. Intracellular amyloid precursor protein sorting and amyloid-? secretion are regulated by Src-mediated phosphorylation of Mint2. J Neurosci. 2012;32:9613-25 pubmed publisher
Alves Silva J, Sánchez Soriano N, Beaven R, Klein M, Parkin J, Millard T, et al. Spectraplakins promote microtubule-mediated axonal growth by functioning as structural microtubule-associated proteins and EB1-dependent +TIPs (tip interacting proteins). J Neurosci. 2012;32:9143-58 pubmed publisher
Ji K, Tsirka S. Inflammation modulates expression of laminin in the central nervous system following ischemic injury. J Neuroinflammation. 2012;9:159 pubmed publisher
Balzer E, Tong Z, Paul C, Hung W, Stroka K, Boggs A, et al. Physical confinement alters tumor cell adhesion and migration phenotypes. FASEB J. 2012;26:4045-56 pubmed publisher
Fujimura A, Kishimoto H, Yanagisawa J, Kimura K. Enhancer of rudimentary homolog (ERH) plays an essential role in the progression of mitosis by promoting mitotic chromosome alignment. Biochem Biophys Res Commun. 2012;423:588-92 pubmed publisher
Wood J, Wang J, Benovic J, Ferkey D. Structural domains required for Caenorhabditis elegans G protein-coupled receptor kinase 2 (GRK-2) function in vivo. J Biol Chem. 2012;287:12634-44 pubmed publisher
Chow W, Luk H, Chan H, Lau K. Degradation of mutant huntingtin via the ubiquitin/proteasome system is modulated by FE65. Biochem J. 2012;443:681-9 pubmed publisher
Nummela P, Lammi J, Soikkeli J, Saksela O, Laakkonen P, Hölttä E. Transforming growth factor beta-induced (TGFBI) is an anti-adhesive protein regulating the invasive growth of melanoma cells. Am J Pathol. 2012;180:1663-74 pubmed publisher
Park C, Ryu H, Ryu K. Locus coeruleus neurons are resistant to dysfunction and degeneration by maintaining free ubiquitin levels although total ubiquitin levels decrease upon disruption of polyubiquitin gene Ubb. Biochem Biophys Res Commun. 2012;418:541-6 pubmed publisher
Killian R, Flippin J, Herrera C, Almenar Queralt A, Goldstein L. Kinesin light chain 1 suppression impairs human embryonic stem cell neural differentiation and amyloid precursor protein metabolism. PLoS ONE. 2012;7:e29755 pubmed publisher
Wright C, Agboke F, Muthu M, Michaelis K, Mundy M, La P, et al. Nuclear factor-?B (NF-?B) inhibitory protein I?B? determines apoptotic cell death following exposure to oxidative stress. J Biol Chem. 2012;287:6230-9 pubmed publisher
Datta S, O Malley M, Patterson E. Calcium/calmodulin kinase II in the pedunculopontine tegmental nucleus modulates the initiation and maintenance of wakefulness. J Neurosci. 2011;31:17007-16 pubmed publisher
Kempkes C, Rattenholl A, Buddenkotte J, Strozyk E, Eberle J, Hausser A, et al. Proteinase-activated receptors 1 and 2 regulate invasive behavior of human melanoma cells via activation of protein kinase D1. J Invest Dermatol. 2012;132:375-84 pubmed publisher
Mitra D, Das P, Huynh F, Jones F. Jumonji/ARID1 B (JARID1B) protein promotes breast tumor cell cycle progression through epigenetic repression of microRNA let-7e. J Biol Chem. 2011;286:40531-5 pubmed publisher
Watanabe T, Urano E, Miyauchi K, Ichikawa R, Hamatake M, Misawa N, et al. The hematopoietic cell-specific Rho GTPase inhibitor ARHGDIB/D4GDI limits HIV type 1 replication. AIDS Res Hum Retroviruses. 2012;28:913-22 pubmed publisher
Lyons P, Fricker L. Carboxypeptidase O is a glycosylphosphatidylinositol-anchored intestinal peptidase with acidic amino acid specificity. J Biol Chem. 2011;286:39023-32 pubmed publisher
Rauert H, Stühmer T, Bargou R, Wajant H, Siegmund D. TNFR1 and TNFR2 regulate the extrinsic apoptotic pathway in myeloma cells by multiple mechanisms. Cell Death Dis. 2011;2:e194 pubmed publisher
Fabrega A, Puigmulé M, Yeste M, Casas I, Bonet S, Pinart E. Impact of epididymal maturation, ejaculation and in vitro capacitation on tyrosine phosphorylation patterns exhibited of boar (Sus domesticus) spermatozoa. Theriogenology. 2011;76:1356-66 pubmed publisher
Kumar S, Park S, Cieply B, Schupp J, Killiam E, Zhang F, et al. A pathway for the control of anoikis sensitivity by E-cadherin and epithelial-to-mesenchymal transition. Mol Cell Biol. 2011;31:4036-51 pubmed publisher
Wang H, Dávila García M, Yarl W, Gondré Lewis M. Gestational nicotine exposure regulates expression of AMPA and NMDA receptors and their signaling apparatus in developing and adult rat hippocampus. Neuroscience. 2011;188:168-81 pubmed publisher
Vega F, Fruhwirth G, Ng T, Ridley A. RhoA and RhoC have distinct roles in migration and invasion by acting through different targets. J Cell Biol. 2011;193:655-65 pubmed publisher
Takasugi N, Sasaki T, Suzuki K, Osawa S, Isshiki H, Hori Y, et al. BACE1 activity is modulated by cell-associated sphingosine-1-phosphate. J Neurosci. 2011;31:6850-7 pubmed publisher
Hennen E, Czopka T, Faissner A. Structurally distinct LewisX glycans distinguish subpopulations of neural stem/progenitor cells. J Biol Chem. 2011;286:16321-31 pubmed publisher
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Desarnaud F, Macone B, Datta S. Activation of extracellular signal-regulated kinase signaling in the pedunculopontine tegmental cells is involved in the maintenance of sleep in rats. J Neurochem. 2011;116:577-87 pubmed publisher
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product information
Catalog Number :
05-829
Subcategory :
Cell Structure
Product Name :
Anti-α-Tubulin Antibody, clone DM1A
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q71U36
Host Name :
Mouse
Antigen :
α-Tubulin
Clone :
DM1A
Conjugate :
Purified
Isotype :
IgG1
Product Description :
Anti-α-Tubulin Antibody, clone DM1A
Cross Reactivity :
Rat;Mouse;Pig;Bovine;Avian;Guinea Pig;Human
Background :
Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.
ALT Names :
Alpha-tubulin 3;Tubulin B-alpha-1;Tubulin alpha-3 chain;tubulin, alpha 1a;tubulin, alpha 3;tubulin, alpha, brain-specific
Immunogen :
Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSVEGEGEEEGEE).
Specificity :
Recognizes α-Tubulin, clone DM1A, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to amino acids 426-450.
Package Size :
200 µg
Uses :
Immunocytochemistry;Immunofluorescence;Western Blotting
Storage :
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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