phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant | EMD Millipore 05-817R-I product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant

catalog :

05-817R-I

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

clone name :

63-1C-8

reactivity :

human

application :

western blot, flow cytometry

citations: 1

Reference
Cheng L, Tu K, Seidel C, Robb S, Guo F, SANCHEZ ALVARADO A. Cellular, ultrastructural and molecular analyses of epidermal cell development in the planarian Schmidtea mediterranea. Dev Biol. 2018;433:357-373 pubmed publisher

product information

Catalog Number :

05-817R-I

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q16695

Host Name :

Rabbit

Antigen :

HIST3H3

Clone :

63-1C-8

Conjugate :

Purified

Purification Method :

Protein A purified

Isotype :

IgGκ

Product Description :

Anti-phospho-Histone H3 (Ser10) Antibody, clone 63-1C-8, Recombinant

Cross Reactivity :

Human

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming the nucleosome. Histones are modified post-translationally; and these modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Phosphorylation at Ser10, which is linked to gene activation, prevents methylation at Lys9 but facilitates acetylation of H3 and H4. Phosphorylated Ser10 is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition, Ser10 phosphorylation is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser10 by Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.

ALT Names :

H3S10p, Histone H3.1t, H3/t, H3/g, histone H3 phosphoserine 10, H3 ser10, H3 serine 10

Immunogen :

KLH-conjugated linear peptide corresponding to Human Histone H3 phosphorylated at Ser10.

Package Size :

100 µL

Uses :

Western Blotting, Dot Blot, Flow Cytometry

Storage :

Stable for 1 year at 2-8°C from date of receipt