HDAC2 Antibody, clone 3F3 | EMD Millipore 05-814 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

HDAC2 Antibody, clone 3F3

catalog :

05-814

quantity :

200 μg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

3F3

The same clone is also sold as:

Abcam: ab51832
Santa Cruz Biotechnology: sc-81599
ImmuQuest: IQ544, IQ544HRP, IQ544FITC

reactivity :

human, mouse, bovine

application :

western blot, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation, western blot knockout validation

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:2000; fig 1	Jamaladdin S, Kelly R, O Regan L, Dovey O, Hodson G, Millard C, et al. Histone deacetylase (HDAC) 1 and 2 are essential for accurate cell division and the pluripotency of embryonic stem cells. Proc Natl Acad Sci U S A. 2014;111:9840-5 pubmed publisher
western blot knockout validation; mouse; 1:10,000; fig 4 immunocytochemistry; mouse; 1:200; fig 1	Ma P, Pan H, Montgomery R, Olson E, Schultz R. Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development. Proc Natl Acad Sci U S A. 2012;109:E481-9 pubmed publisher
immunocytochemistry; mouse; loading ...; fig s6a	Fukuda A, Mitani A, Miyashita T, Umezawa A, Akutsu H. Chromatin condensation of Xist genomic loci during oogenesis in mice. Development. 2015;142:4049-55 pubmed publisher
chromatin immunoprecipitation; human	Lezina L, Aksenova V, Ivanova T, Purmessur N, Antonov A, Tentler D, et al. KMTase Set7/9 is a critical regulator of E2F1 activity upon genotoxic stress. Cell Death Differ. 2014;21:1889-99 pubmed publisher
western blot; mouse; fig 1	Xu Y, Swartz K, Siu K, Bhattacharyya M, Minella A. Fbw7-dependent cyclin E regulation ensures terminal maturation of bone marrow erythroid cells by restraining oxidative metabolism. Oncogene. 2014;33:3161-71 pubmed publisher
chromatin immunoprecipitation; human	Corvetta D, Chayka O, Gherardi S, D Acunto C, Cantilena S, Valli E, et al. Physical interaction between MYCN oncogene and polycomb repressive complex 2 (PRC2) in neuroblastoma: functional and therapeutic implications. J Biol Chem. 2013;288:8332-41 pubmed publisher
chromatin immunoprecipitation; human western blot; human	Milagre I, Nunes M, Moutinho M, Rivera I, Fuso A, Scarpa S, et al. Chromatin-modifying agents increase transcription of CYP46A1, a key player in brain cholesterol elimination. J Alzheimers Dis. 2010;22:1209-21 pubmed publisher
western blot; mouse	Foster C, Dovey O, Lezina L, Luo J, Gant T, Barlev N, et al. Lysine-specific demethylase 1 regulates the embryonic transcriptome and CoREST stability. Mol Cell Biol. 2010;30:4851-63 pubmed publisher
	Roy M, Mukherjee S. Reversal of resistance towards cisplatin by curcumin in cervical cancer cells. Asian Pac J Cancer Prev. 2014;15:1403-10 pubmed
	Liu L, Karmakar S, Dhar R, Mahajan M, Choudhury A, Weissman S, et al. Regulation of G?-globin gene by ATF2 and its associated proteins through the cAMP-response element. PLoS ONE. 2013;8:e78253 pubmed publisher
	Gnatovskiy L, Mita P, Levy D. The human RVB complex is required for efficient transcription of type I interferon-stimulated genes. Mol Cell Biol. 2013;33:3817-25 pubmed publisher
	Palavicini J, Wang H, Bianchi E, Xu S, Rao J, Kang D, et al. RanBP9 aggravates synaptic damage in the mouse brain and is inversely correlated to spinophilin levels in Alzheimer's brain synaptosomes. Cell Death Dis. 2013;4:e667 pubmed publisher
	Waby J, Chirakkal H, Yu C, Griffiths G, Benson R, Bingle C, et al. Sp1 acetylation is associated with loss of DNA binding at promoters associated with cell cycle arrest and cell death in a colon cell line. Mol Cancer. 2010;9:275 pubmed publisher
	Ma P, Lin S, Bartolomei M, Schultz R. Metastasis tumor antigen 2 (MTA2) is involved in proper imprinted expression of H19 and Peg3 during mouse preimplantation development. Biol Reprod. 2010;83:1027-35 pubmed publisher
	Bhandari D, Seo K, Jung J, Kim H, Yang S, Kang K. The regulatory role of c-MYC on HDAC2 and PcG expression in human multipotent stem cells. J Cell Mol Med. 2011;15:1603-14 pubmed publisher
	Ku W, Chiu S, Chen Y, Huang H, Wu W, Chen Y. Complementary quantitative proteomics reveals that transcription factor AP-4 mediates E-box-dependent complex formation for transcriptional repression of HDM2. Mol Cell Proteomics. 2009;8:2034-50 pubmed publisher
	Chabane N, Li X, Fahmi H. HDAC4 contributes to IL-1-induced mPGES-1 expression in human synovial fibroblasts through up-regulation of Egr-1 transcriptional activity. J Cell Biochem. 2009;106:453-63 pubmed publisher

product information

Catalog Number :

05-814

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-HDAC2 Antibody, clone 3F3

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

Q92769

Host Name :

Mouse

Antigen :

HDAC2

Clone :

3F3

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-HDAC2 Antibody, clone 3F3

Cross Reactivity :

Human;Bovine;Mouse;Rat;Hamster

Background :

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

ALT Names :

YY1-associated factor 1;histone deacetylase 2;transcriptional regulator homolog RPD3

Immunogen :

KLH-conjugated, synthetic peptide (CEKTDTKGTKSEQLSNP) corresponding to amino acids 473-488 of human HDAC2 (Accession # NP_001518) containing a N-terminal cysteine

Specificity :

Recognizes HDAC2, MW ~60 kDa.

Package Size :

200 μg

Uses :

Immunocytochemistry;Immunoprecipitation;Western Blotting

Storage :

Stable for 1 year at -20ºC from date of receipt.