This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
EMD Millipore
other brands :
Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava
product type :
antibody
product name :
phospho-Histone H3 (Ser10) Antibody, clone 3H10
catalog :
05-806
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
phosphorylated
clone name :
3H10
reactivity :
geckos , Chinese softshell turtle, human, mouse, rat, chicken, zebrafish , Xenopus laevis
application :
western blot, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
citations: 60
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; rat; 1:10,000; loading ...; fig s3a
Nomura Komoike K, Saitoh F, Fujieda H. Phosphatidylserine recognition and Rac1 activation are required for Müller glia proliferation, gliosis and phagocytosis after retinal injury. Sci Rep. 2020;10:1488 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:200; loading ...; fig s40c
Kim J, Fei L, Yin W, Coquenlorge S, Rao Bhatia A, Zhang X, et al. Single cell and genetic analyses reveal conserved populations and signaling mechanisms of gastrointestinal stromal niches. Nat Commun. 2020;11:334 pubmed publisher
  • immunohistochemistry - frozen section; mouse; 1:3000; loading ...; fig 3a
  • immunohistochemistry - frozen section; human; 1:3000; loading ...; fig 6c
Bendriem R, Singh S, Aleem A, Antonetti D, Ross M. Tight junction protein occludin regulates progenitor Self-Renewal and survival in developing cortex. elife. 2019;8: pubmed publisher
  • flow cytometry; human; 1:500; loading ...; fig s1b
Allan L, Skowyra A, Rogers K, Zeller D, Clarke P. Atypical APC/C-dependent degradation of Mcl-1 provides an apoptotic timer during mitotic arrest. EMBO J. 2018;37: pubmed publisher
  • immunohistochemistry; mouse; loading ...; fig 1g
Kim J, Kim Y, Kim J, Park D, Bae H, Lee D, et al. YAP/TAZ regulates sprouting angiogenesis and vascular barrier maturation. J Clin Invest. 2017;127:3441-3461 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:1000; loading ...; fig 3a
Freddo A, Shoffner S, Shao Y, Taniguchi K, Grosse A, Guysinger M, et al. Coordination of signaling and tissue mechanics during morphogenesis of murine intestinal villi: a role for mitotic cell rounding. Integr Biol (Camb). 2016;8:918-28 pubmed publisher
  • immunohistochemistry - frozen section; zebrafish ; 1:200; fig 3
Sallin P, Jazwinska A. Acute stress is detrimental to heart regeneration in zebrafish. Open Biol. 2016;6: pubmed publisher
  • western blot; human; fig 3
Lee S, Jeong A, Park J, Han S, Jang C, Kim K, et al. IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells. Cell Mol Life Sci. 2016;73:3375-86 pubmed publisher
  • flow cytometry; human; fig 2
Hasvold G, Lund Andersen C, Lando M, Patzke S, Hauge S, Suo Z, et al. Hypoxia-induced alterations of G2 checkpoint regulators. Mol Oncol. 2016;10:764-73 pubmed publisher
  • immunohistochemistry - frozen section; chicken; 1:500; fig 1
  • immunohistochemistry - frozen section; mouse; 1:500; fig 1
  • immunohistochemistry - frozen section; Xenopus laevis; 1:500; fig 4
Nomura T, Ohtaka Maruyama C, Yamashita W, Wakamatsu Y, Murakami Y, Calegari F, et al. The evolution of basal progenitors in the developing non-mammalian brain. Development. 2016;143:66-74 pubmed publisher
  • immunocytochemistry; human; loading ...; fig s1d
Yan K, Li L, Wang X, Hong R, Zhang Y, Yang H, et al. The deubiquitinating enzyme complex BRISC is required for proper mitotic spindle assembly in mammalian cells. J Cell Biol. 2015;210:209-24 pubmed publisher
  • western blot; human
Xu H, Dephoure N, Sun H, Zhang H, Fan F, Liu J, et al. Proteomic Profiling of Paclitaxel Treated Cells Identifies a Novel Mechanism of Drug Resistance Mediated by PDCD4. J Proteome Res. 2015;14:2480-91 pubmed publisher
  • flow cytometry; mouse; 1:500; fig 7
Czechanski A, Kim H, Byers C, Greenstein I, Stumpff J, Reinholdt L. Kif18a is specifically required for mitotic progression during germ line development. Dev Biol. 2015;402:253-262 pubmed publisher
  • immunohistochemistry; Chinese softshell turtle; 1:500; fig 5c
  • immunohistochemistry; geckos ; 1:500; loading ...; fig 5c
Nomura T, Yamashita W, Gotoh H, Ono K. Genetic manipulation of reptilian embryos: toward an understanding of cortical development and evolution. Front Neurosci. 2015;9:45 pubmed publisher
  • immunocytochemistry; mouse
Carter E, Miron Buchacra G, Goldoni S, Danahay H, Westwick J, Watson M, et al. Phosphoinositide 3-kinase alpha-dependent regulation of branching morphogenesis in murine embryonic lung: evidence for a role in determining morphogenic properties of FGF7. PLoS ONE. 2014;9:e113555 pubmed publisher
  • immunocytochemistry; human
Petazzi P, Akizu N, Garcia A, Estarás C, Martinez de Paz A, Rodríguez Paredes M, et al. An increase in MECP2 dosage impairs neural tube formation. Neurobiol Dis. 2014;67:49-56 pubmed publisher
  • western blot; human
Matthess Y, Raab M, Knecht R, Becker S, Strebhardt K. Sequential Cdk1 and Plk1 phosphorylation of caspase-8 triggers apoptotic cell death during mitosis. Mol Oncol. 2014;8:596-608 pubmed publisher
  • immunocytochemistry; mouse; 1:250
Stewart C, Wang Y, Bonilla Claudio M, Martin J, Gonzalez G, Taketo M, et al. CTNNB1 in mesenchyme regulates epithelial cell differentiation during Müllerian duct and postnatal uterine development. Mol Endocrinol. 2013;27:1442-54 pubmed publisher
  • western blot; human; 0.5 ug/ml
Egelhofer T, Minoda A, Klugman S, Lee K, Kolasinska Zwierz P, Alekseyenko A, et al. An assessment of histone-modification antibody quality. Nat Struct Mol Biol. 2011;18:91-3 pubmed publisher
Franco M, Carmena A. Eph signaling controls mitotic spindle orientation and cell proliferation in neuroepithelial cells. J Cell Biol. 2019;: pubmed publisher
Watts E, Heidenreich D, Tucker E, Raab M, Strebhardt K, Chesler L, et al. Designing Dual Inhibitors of Anaplastic Lymphoma Kinase (ALK) and Bromodomain-4 (BRD4) by Tuning Kinase Selectivity. J Med Chem. 2019;62:2618-2637 pubmed publisher
Marshall L, Vivien C, Girardot F, Péricard L, Scerbo P, Palmier K, et al. Stage-dependent cardiac regeneration in Xenopus is regulated by thyroid hormone availability. Proc Natl Acad Sci U S A. 2019;116:3614-3623 pubmed publisher
Zhang M, Di Martino J, Bowman R, Campbell N, Baksh S, Simon Vermot T, et al. Adipocyte-Derived Lipids Mediate Melanoma Progression via FATP Proteins. Cancer Discov. 2018;8:1006-1025 pubmed publisher
Zhang Y, Zimmer M, Guardia T, Callahan S, Mondal C, Di Martino J, et al. Distant Insulin Signaling Regulates Vertebrate Pigmentation through the Sheddase Bace2. Dev Cell. 2018;45:580-594.e7 pubmed publisher
Liu W, Cvekl A. Six3 in a small population of progenitors at E8.5 is required for neuroretinal specification via regulating cell signaling and survival in mice. Dev Biol. 2017;428:164-175 pubmed publisher
Fini J, Mughal B, Le Mevel S, Leemans M, Lettmann M, Spirhanzlova P, et al. Human amniotic fluid contaminants alter thyroid hormone signalling and early brain development in Xenopus embryos. Sci Rep. 2017;7:43786 pubmed publisher
Haushalter C, Schuhbaur B, Dolle P, Rhinn M. Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development. Biol Open. 2017;6:148-160 pubmed publisher
Traboulsi H, Davoli S, Catez P, Egly J, Compe E. Dynamic partnership between TFIIH, PGC-1α and SIRT1 is impaired in trichothiodystrophy. PLoS Genet. 2014;10:e1004732 pubmed publisher
Kogata N, Oliemuller E, Wansbury O, Howard B. Neuregulin-3 regulates epithelial progenitor cell positioning and specifies mammary phenotype. Stem Cells Dev. 2014;23:2758-70 pubmed publisher
Wohlan K, Goy S, Olling A, Srivaratharajan S, Tatge H, Genth H, et al. Pyknotic cell death induced by Clostridium difficile TcdB: chromatin condensation and nuclear blister are induced independently of the glucosyltransferase activity. Cell Microbiol. 2014;16:1678-92 pubmed publisher
VanLeeuwen J, Rafalovich I, Sellers K, Jones K, Griffith T, Huda R, et al. Coordinated nuclear and synaptic shuttling of afadin promotes spine plasticity and histone modifications. J Biol Chem. 2014;289:10831-42 pubmed publisher
McGregor M, Hariharan N, Joyo A, Margolis R, Sussman M. CENP-A is essential for cardiac progenitor cell proliferation. Cell Cycle. 2014;13:739-48 pubmed publisher
Borowiec A, Bidaux G, Tacine R, Dubar P, Pigat N, Delcourt P, et al. Are Orai1 and Orai3 channels more important than calcium influx for cell proliferation?. Biochim Biophys Acta. 2014;1843:464-72 pubmed publisher
Perez de Castro I, Aguirre Portolés C, Fernández Miranda G, Canamero M, Cowley D, van Dyke T, et al. Requirements for Aurora-A in tissue regeneration and tumor development in adult mammals. Cancer Res. 2013;73:6804-15 pubmed publisher
Cabon L, Sriskandarajah N, Mui M, Teodoro J, Blanchette P, Branton P. Adenovirus E4orf4 protein-induced death of p53-/- H1299 human cancer cells follows a G1 arrest of both tetraploid and diploid cells due to a failure to initiate DNA synthesis. J Virol. 2013;87:13168-78 pubmed publisher
Ahn H, Choi J, Kim K, Kim H, Choi S, Park S, et al. Phase II study of pazopanib monotherapy in metastatic gastroenteropancreatic neuroendocrine tumours. Br J Cancer. 2013;109:1414-9 pubmed publisher
Marques L, Thorsteinsdóttir S. Dynamics of Akt activation during mouse embryo development: distinct subcellular patterns distinguish proliferating versus differentiating cells. Differentiation. 2013;86:48-56 pubmed publisher
Iovino N, Ciabrelli F, Cavalli G. PRC2 controls Drosophila oocyte cell fate by repressing cell cycle genes. Dev Cell. 2013;26:431-9 pubmed publisher
Kogata N, Howard B. A whole-mount immunofluorescence protocol for three-dimensional imaging of the embryonic mammary primordium. J Mammary Gland Biol Neoplasia. 2013;18:227-31 pubmed publisher
Gurudatta B, Yang J, Van Bortle K, Donlin Asp P, Corces V. Dynamic changes in the genomic localization of DNA replication-related element binding factor during the cell cycle. Cell Cycle. 2013;12:1605-15 pubmed publisher
Chen J, Krasnow M. Integrin Beta 1 suppresses multilayering of a simple epithelium. PLoS ONE. 2012;7:e52886 pubmed publisher
Shan W, Akinfenwa P, Savannah K, Kolomeyevskaya N, Laucirica R, Thomas D, et al. A small-molecule inhibitor targeting the mitotic spindle checkpoint impairs the growth of uterine leiomyosarcoma. Clin Cancer Res. 2012;18:3352-65 pubmed publisher
Kellner W, Ramos E, Van Bortle K, Takenaka N, Corces V. Genome-wide phosphoacetylation of histone H3 at Drosophila enhancers and promoters. Genome Res. 2012;22:1081-8 pubmed publisher
Ren K, Lu J, Porollo A, Du C. Tumor-suppressing function of caspase-2 requires catalytic site Cys-320 and site Ser-139 in mice. J Biol Chem. 2012;287:14792-802 pubmed publisher
Burns K, Gutzwiller L, Tomoyasu Y, Gebelein B. Oenocyte development in the red flour beetle Tribolium castaneum. Dev Genes Evol. 2012;222:77-88 pubmed publisher
Dronamraju R, Mason J. MU2 and HP1a regulate the recognition of double strand breaks in Drosophila melanogaster. PLoS ONE. 2011;6:e25439 pubmed publisher
Yoshida M, Feng L, Grimbert F, Rangarajan K, Buggele W, Copenhagen D, et al. Overexpression of neurotrophin-3 stimulates a second wave of dopaminergic amacrine cell genesis after birth in the mouse retina. J Neurosci. 2011;31:12663-73 pubmed publisher
Grosse A, Pressprich M, Curley L, Hamilton K, Margolis B, Hildebrand J, et al. Cell dynamics in fetal intestinal epithelium: implications for intestinal growth and morphogenesis. Development. 2011;138:4423-32 pubmed publisher
Winter G, Rix U, Lissat A, Stukalov A, Müllner M, Bennett K, et al. An integrated chemical biology approach identifies specific vulnerability of Ewing's sarcoma to combined inhibition of Aurora kinases A and B. Mol Cancer Ther. 2011;10:1846-56 pubmed publisher
Sánchez Molina S, Mortusewicz O, Bieber B, Auer S, Eckey M, Leonhardt H, et al. Role for hACF1 in the G2/M damage checkpoint. Nucleic Acids Res. 2011;39:8445-56 pubmed publisher
Stewart C, Fisher S, Wang Y, Stewart M, Hewitt S, Rodriguez K, et al. Uterine gland formation in mice is a continuous process, requiring the ovary after puberty, but not after parturition. Biol Reprod. 2011;85:954-64 pubmed publisher
Sashida G, Bae N, Di Giandomenico S, Asai T, Gurvich N, Bazzoli E, et al. The mef/elf4 transcription factor fine tunes the DNA damage response. Cancer Res. 2011;71:4857-65 pubmed publisher
Koning M, Werker P, van Luyn M, Harmsen M. Hypoxia promotes proliferation of human myogenic satellite cells: a potential benefactor in tissue engineering of skeletal muscle. Tissue Eng Part A. 2011;17:1747-58 pubmed publisher
Matthess Y, Raab M, Sanhaji M, Lavrik I, Strebhardt K. Cdk1/cyclin B1 controls Fas-mediated apoptosis by regulating caspase-8 activity. Mol Cell Biol. 2010;30:5726-40 pubmed publisher
Suzuki K, Nakashima M, Yamashita S. Dynamics of ionizing radiation-induced DNA damage response in reconstituted three-dimensional human skin tissue. Radiat Res. 2010;174:415-23 pubmed publisher
Ciccia A, Bredemeyer A, Sowa M, Terret M, Jallepalli P, Harper J, et al. The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart. Genes Dev. 2009;23:2415-25 pubmed publisher
Reed J, Leon R, Hall M, Schwertfeger K. Interleukin-1beta and fibroblast growth factor receptor 1 cooperate to induce cyclooxygenase-2 during early mammary tumourigenesis. Breast Cancer Res. 2009;11:R21 pubmed publisher
Oda H, Okamoto I, Murphy N, Chu J, Price S, Shen M, et al. Monomethylation of histone H4-lysine 20 is involved in chromosome structure and stability and is essential for mouse development. Mol Cell Biol. 2009;29:2278-95 pubmed publisher
Pomerening J, Ubersax J, Ferrell J. Rapid cycling and precocious termination of G1 phase in cells expressing CDK1AF. Mol Biol Cell. 2008;19:3426-41 pubmed publisher
Mitra S, Mikolon D, Molina J, Hsia D, Hanson D, Chi A, et al. Intrinsic FAK activity and Y925 phosphorylation facilitate an angiogenic switch in tumors. Oncogene. 2006;25:5969-84 pubmed
product information
Catalog Number :
05-806
Subcategory :
Epigenetics & Nuclear Function
Product Name :
Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10
Product Type :
Antibodies
Clonality :
Monoclonal Antibody
Gene ID :
Q16695
Host Name :
Mouse
Antigen :
phospho-Histone H3 (Ser10)
Clone :
3H10
Conjugate :
Purified
Isotype :
IgG1κ
Product Description :
Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10
Cross Reactivity :
Human
Background :
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
ALT Names :
H3S10P;Histone H3 (phospho S10);H3 histone family, member T;histone 3, H3;histone cluster 3, H3
Immunogen :
A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3.
Specificity :
Histone H3 phosphorylated at Ser10.
Package Size :
100 µg
Uses :
Flow Cytometry;Immunocytochemistry;Immunofluorescence;Peptide Inhibition Assay;Western Blotting;Multiplexing
Storage :
Stable for 1 year at 2 to 8°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
company information
EMD Millipore
290 Concord Road
Billerica, Massachusetts 01821
bioscienceshelp@emdchemical.com
https://www.emdmillipore.com
888-854-3417
headquarters: United States
EMD Millipore is the Life Science division of Merck KGaA of Darmstadt, Germany

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