Tau (4-repeat isoform RD4) Antibody, clone 1E1/A6 | EMD Millipore 05-804 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Tau (4-repeat isoform RD4) Antibody, clone 1E1/A6

catalog :

05-804

quantity :

200 μL

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

1E1/A6

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, western blot knockout validation

citations: 30

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; 1:500; tbl 1 immunocytochemistry; human; 1:50; tbl 2 western blot; mouse	Ercan E, Eid S, Weber C, Kowalski A, Bichmann M, Behrendt A, et al. A validated antibody panel for the characterization of tau post-translational modifications. Mol Neurodegener. 2017;12:87 pubmed publisher
immunohistochemistry; mouse; 1:10,000; loading ...; fig 2, 4, 7	He Z, McBride J, Xu H, Changolkar L, Kim S, Zhang B, et al. Transmission of tauopathy strains is independent of their isoform composition. Nat Commun. 2020;11:7 pubmed publisher
immunohistochemistry - paraffin section; human; 1:4000; loading ...; fig 2a10	Shafei R, Woollacott I, Mummery C, Bocchetta M, Guerreiro R, Bras J, et al. Two pathologically confirmed cases of novel mutations in the MAPT gene causing frontotemporal dementia. Neurobiol Aging. 2020;87:141.e15-141.e20 pubmed publisher
immunohistochemistry - paraffin section; human; 1:1000; loading ...; fig 5c, 5i	Pires G, McElligott S, Drusinsky S, Halliday G, Potier M, Wisniewski T, et al. Secernin-1 is a novel phosphorylated tau binding protein that accumulates in Alzheimer's disease and not in other tauopathies. Acta Neuropathol Commun. 2019;7:195 pubmed publisher
immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1i	Stejskalova Z, Rohan Z, Rusina R, Tesar A, Kukal J, Kovacs G, et al. Pyramidal system involvement in progressive supranuclear palsy - a clinicopathological correlation. BMC Neurol. 2019;19:42 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50; loading ...	van der Zee J, Gijselinck I, Van Mossevelde S, Perrone F, Dillen L, Heeman B, et al. TBK1 Mutation Spectrum in an Extended European Patient Cohort with Frontotemporal Dementia and Amyotrophic Lateral Sclerosis. Hum Mutat. 2017;38:297-309 pubmed publisher
western blot; mouse; 1:1000; fig 2	Manassero G, Guglielmotto M, Zamfir R, Borghi R, Colombo L, Salmona M, et al. Beta-amyloid 1-42 monomers, but not oligomers, produce PHF-like conformation of Tau protein. Aging Cell. 2016;15:914-23 pubmed publisher
immunohistochemistry - paraffin section; human; 1:50; loading ...	Gelpi E, Hoftberger R, Graus F, Ling H, Holton J, Dawson T, et al. Neuropathological criteria of anti-IgLON5-related tauopathy. Acta Neuropathol. 2016;132:531-43 pubmed publisher
immunohistochemistry - paraffin section; human; fig 3	Ando K, Tomimura K, Sazdovitch V, Suain V, Yilmaz Z, Authelet M, et al. Level of PICALM, a key component of clathrin-mediated endocytosis, is correlated with levels of phosphotau and autophagy-related proteins and is associated with tau inclusions in AD, PSP and Pick disease. Neurobiol Dis. 2016;94:32-43 pubmed publisher
western blot; mouse; 1:1000; fig 12	Polanco J, Scicluna B, Hill A, Götz J. Extracellular Vesicles Isolated from the Brains of rTg4510 Mice Seed Tau Protein Aggregation in a Threshold-dependent Manner. J Biol Chem. 2016;291:12445-66 pubmed publisher
immunohistochemistry - paraffin section; human; 1:100; fig 3	Nishikawa T, Takahashi T, Nakamori M, Hosomi N, Maruyama H, Miyazaki Y, et al. The identification of raft-derived tau-associated vesicles that are incorporated into immature tangles and paired helical filaments. Neuropathol Appl Neurobiol. 2016;42:639-653 pubmed publisher
immunohistochemistry; human; 1:250; fig s4	Ziskin J, Greicius M, Zhu W, Okumu A, Adams C, Plowey E. Neuropathologic analysis of Tyr69His TTR variant meningovascular amyloidosis with dementia. Acta Neuropathol Commun. 2015;3:43 pubmed publisher
western blot; human; 1:1,000 immunohistochemistry - paraffin section; mouse; 1:80 western blot; mouse; 1:1,000	Fernández Nogales M, Cabrera J, Santos Galindo M, Hoozemans J, Ferrer I, Rozemuller A, et al. Huntington's disease is a four-repeat tauopathy with tau nuclear rods. Nat Med. 2014;20:881-5 pubmed publisher
western blot; mouse; 1:1000	Liu C, Götz J. Profiling murine tau with 0N, 1N and 2N isoform-specific antibodies in brain and peripheral organs reveals distinct subcellular localization, with the 1N isoform being enriched in the nucleus. PLoS ONE. 2013;8:e84849 pubmed publisher
western blot; mouse; 1:1000; fig 10 immunohistochemistry - paraffin section; human; 1:80	McMillan P, Korvatska E, Poorkaj P, Evstafjeva Z, Robinson L, Greenup L, et al. Tau isoform regulation is region- and cell-specific in mouse brain. J Comp Neurol. 2008;511:788-803 pubmed publisher
	Kurihara M, Mano T, Saito Y, Murayama S, Toda T, Iwata A. Colocalization of BRCA1 with Tau Aggregates in Human Tauopathies. Brain Sci. 2019;10: pubmed publisher
	Julian M, Rabia L, Desai A, Arsiwala A, Gerson J, Paulson H, et al. Nature-inspired design and evolution of anti-amyloid antibodies. J Biol Chem. 2019;294:8438-8451 pubmed publisher
	Cliffe R, Sang J, Kundel F, Finley D, Klenerman D, Ye Y. Filamentous Aggregates Are Fragmented by the Proteasome Holoenzyme. Cell Rep. 2019;26:2140-2149.e3 pubmed publisher
	Schirer Y, Malishkevich A, Ophir Y, Lewis J, Giladi E, Gozes I. Novel marker for the onset of frontotemporal dementia: early increase in activity-dependent neuroprotective protein (ADNP) in the face of Tau mutation. PLoS ONE. 2014;9:e87383 pubmed publisher
	Gelpi E, Navarro Otano J, Tolosa E, Gaig C, Compta Y, Rey M, et al. Multiple organ involvement by alpha-synuclein pathology in Lewy body disorders. Mov Disord. 2014;29:1010-8 pubmed publisher
	Clerc M, Deprez M, Leuba G, Lhermitte B, Lopez U, von Gunten A. Atypical association of semantic dementia, corticobasal syndrome, and 4R tauopathy. Neurocase. 2015;21:1-15 pubmed publisher
	Grau Rivera O, Gelpi E, Rey M, Valldeoriola F, Tolosa E, Compta Y, et al. Prominent psychiatric symptoms in patients with Parkinson's disease and concomitant argyrophilic grain disease. J Neurol. 2013;260:3002-9 pubmed publisher
	King A, Al Sarraj S, Troakes C, Smith B, Maekawa S, Iovino M, et al. Mixed tau, TDP-43 and p62 pathology in FTLD associated with a C9ORF72 repeat expansion and p.Ala239Thr MAPT (tau) variant. Acta Neuropathol. 2013;125:303-10 pubmed publisher
	Gelpi E, Lladó A, Clarimon J, Rey M, Rivera R, Ezquerra M, et al. Phenotypic variability within the inclusion body spectrum of basophilic inclusion body disease and neuronal intermediate filament inclusion disease in frontotemporal lobar degenerations with FUS-positive inclusions. J Neuropathol Exp Neurol. 2012;71:795-805 pubmed publisher
	Colom Cadena M, Gelpi E, Marti M, Charif S, Dols Icardo O, Blesa R, et al. MAPT H1 haplotype is associated with enhanced ?-synuclein deposition in dementia with Lewy bodies. Neurobiol Aging. 2013;34:936-42 pubmed publisher
	Orozco D, Tahirovic S, Rentzsch K, Schwenk B, Haass C, Edbauer D. Loss of fused in sarcoma (FUS) promotes pathological Tau splicing. EMBO Rep. 2012;13:759-64 pubmed publisher
	Vilas D, Marti M, Botta Orfila T, Colom Cadena M, Gelpi E. Pick's pathology in Parkinson's disease with dementia. Neuropathol Appl Neurobiol. 2012;38:737-43 pubmed publisher
	Yoshida Y, Nunomura J, Shimohata T, Nanjo H, Miyata H. Benign hereditary chorea 2: pathological findings in an autopsy case. Neuropathology. 2012;32:557-65 pubmed publisher
	Antonell A, Gelpi E, Sanchez Valle R, Martinez R, Molinuevo J, Lladó A. Breakpoint sequence analysis of an A?PP locus duplication associated with autosomal dominant Alzheimer's disease and severe cerebral amyloid angiopathy. J Alzheimers Dis. 2012;28:303-8 pubmed publisher
	Smith P, Delay C, Girard J, Papon M, Planel E, Sergeant N, et al. MicroRNA-132 loss is associated with tau exon 10 inclusion in progressive supranuclear palsy. Hum Mol Genet. 2011;20:4016-24 pubmed publisher

product information

Catalog Number :

05-804

Subcategory :

Neuroscience

Product Name :

Anti-Tau (4-repeat isoform RD4) Antibody, clone 1E1/A6

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P10636

Host Name :

Mouse

Antigen :

Tau (4-repeat isoform RD4)

Clone :

1E1/A6

Conjugate :

Culture Supernatant

Isotype :

IgG

Product Description :

Anti-Tau (4-repeat isoform RD4) Antibody, clone 1E1/A6

Cross Reactivity :

Human;Rat;Bovine;Mouse

Background :

Microtubule Associated Proteins, or MAPS, bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosporylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, I, II. Type II MAP includes MAP2, MAP4, and tau and are found in nervous tissue. Six tau isoforms exist in brain tissue, and they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (allowing it to bind to the negatively-charged microtubule). The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.

Immunogen :

Bovine thyroglobulin-conjugated synthetic peptide corresponding to amino acids 275-291 (VQIINKKLDLSNVQSKC) of human Tau (4-repeat isoform RD4). Region is flanking junction coded by adjacent exons 9 & 11 with the inclusion of exon 10. The immunizing sequence is identical in human, rat, mouse and bovine.

Specificity :

Recognizes Tau (4-repeat isoform RD4), Mr 45-65 kDa. Higher MW band (68-72 kDa) represents phosphorylated Tau.

Package Size :

200 μL

Uses :

Immunohistochemistry;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.