phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal | EMD Millipore 05-802R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal

catalog :

05-802R

quantity :

100 μL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

NL50

reactivity :

human, mouse, chicken, bovine

application :

western blot, ELISA, immunocytochemistry

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 5	D Hulst G, Sylow L, Hespel P, Deldicque L. Acute systemic insulin intolerance does not alter the response of the Akt/GSK-3 pathway to environmental hypoxia in human skeletal muscle. Eur J Appl Physiol. 2015;115:1219-31 pubmed publisher

product information

Catalog Number :

05-802R

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P31749

Host Name :

Rabbit

Antigen :

phospho-Akt (Thr308)

Clone :

NL50

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-phospho-Akt (Thr308) Antibody, clone 50-1C-25, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rabbit;Bovine;Chicken

Background :

Akt/PKB is a Ser/Thr kinase and a major known effecter of the PI3 Kinase pathway. It is involved in multiple signaling pathways that relate to many biological processes including metabolism, apoptosis, cell cycle control, angiogenesis, differentiation, and cell growth and proliferation. In mammals, three isoforms of Akt (Akt1/PKCα, Akt2/PKBβ, and Akt3/PKBγ) exists. They exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt1 is the predominant isoform that is in most tissues and is thought to have a dominant role in growth, survival, embryonic development, and adipocyte differentiation. Akt2 is correlated with the regulation of glucose homeostasis and is the predominant isoform expressed in insulin-responsive tissues. Akt3 is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal Pleckstrin Homology (PH) domain that provides a lipid-binding module, a central catalytic domain containing Thr308, and a C-terminal hydrophobic motif containing Ser473. The activation of AKT is dependent on a dual regulatory mechanism that requires both its translocation to the plasma membrane and dual phosphorylation on Thr308 and Ser473 by PDK1 and the TORC2 complex, respectively.

ALT Names :

Protein kinase B;RAC-alpha serine/threonine-protein kinase ;murine thymoma viral (v-akt) oncogene homolog-1;rac protein kinase alpha ;v-akt murine thymoma viral oncogene homolog 1

Immunogen :

KLH-conjugated synthetic peptide containing the Thr308 sequence and surrounding amino acids of human, mouse, rat, bovine and chicken Akt1.

Specificity :

Detects Akt only when phosphorylated on Thr308.

Package Size :

100 μL

Uses :

Western Blotting;Immunofluorescence;Immunocytochemistry;ELISA

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.