phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonal | EMD Millipore 05-797R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonal

catalog :

05-797R

quantity :

100 μL

clonality :

recombinant

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

AW39R

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:1000; loading ...; fig 5b	Ferland D, Darios E, Neubig R, Sjögren B, Truong N, Torres R, et al. Chemerin-induced arterial contraction is Gi- and calcium-dependent. Vascul Pharmacol. 2017;88:30-41 pubmed publisher
western blot; human; loading ...	Waters A, Stewart J, Atigadda V, Mroczek Musulman E, Muccio D, Grubbs C, et al. Preclinical Evaluation of UAB30 in Pediatric Renal and Hepatic Malignancies. Mol Cancer Ther. 2016;15:911-21 pubmed publisher
western blot; human; fig 1a western blot; mouse; fig 1b	O Sullivan C, Schubart A, Mir A, Dev K. The dual S1PR1/S1PR5 drug BAF312 (Siponimod) attenuates demyelination in organotypic slice cultures. J Neuroinflammation. 2016;13:31 pubmed publisher
western blot; rat; 1:1000; fig 2e	Chen K, Tsai M, Wu C, Jou M, Wei I, Huang C. AMPA Receptor-mTOR Activation is Required for the Antidepressant-Like Effects of Sarcosine during the Forced Swim Test in Rats: Insertion of AMPA Receptor may Play a Role. Front Behav Neurosci. 2015;9:162 pubmed publisher
western blot; human	Waters A, Stewart J, Atigadda V, Mroczek Musulman E, Muccio D, Grubbs C, et al. Preclinical Evaluation of a Novel RXR Agonist for the Treatment of Neuroblastoma. Mol Cancer Ther. 2015;14:1559-69 pubmed publisher
immunocytochemistry; mouse	Rutkowska A, Preuss I, Gessier F, Sailer A, Dev K. EBI2 regulates intracellular signaling and migration in human astrocyte. Glia. 2015;63:341-51 pubmed publisher
	Pan C, Chen C, Shih C, Wang M, Wang J, Wu C. Oxidative stress-induced cellular senescence desensitizes cell growth and migration of vascular smooth muscle cells through down-regulation of platelet-derived growth factor receptor-beta. Aging (Albany NY). 2019;11:8085-8102 pubmed publisher
	Arcuri L, Novello S, Frassineti M, Mercatelli D, Pisano C, Morella I, et al. Antiparkinsonian and antidyskinetic profiles of two novel potent and selective nociceptin/orphanin FQ receptor agonists. Br J Pharmacol. 2017;: pubmed publisher
	Pahng A, Paulsen R, McGinn M, Edwards K, Edwards S. Neurobiological Correlates of Pain Avoidance-Like Behavior in Morphine-Dependent and Non-Dependent Rats. Neuroscience. 2017;366:1-14 pubmed publisher
	Isham C, Netzel B, Bossou A, Milosevic D, Cradic K, Grebe S, et al. Development and characterization of a differentiated thyroid cancer cell line resistant to VEGFR-targeted kinase inhibitors. J Clin Endocrinol Metab. 2014;99:E936-43 pubmed publisher
	Megison M, Gillory L, Stewart J, Nabers H, Mrozcek Musulman E, Beierle E. FAK inhibition abrogates the malignant phenotype in aggressive pediatric renal tumors. Mol Cancer Res. 2014;12:514-26 pubmed publisher
	Hayes H, Moss L, Schisler J, Haldeman J, Zhang Z, Rosenberg P, et al. Pdx-1 activates islet ?- and ?-cell proliferation via a mechanism regulated by transient receptor potential cation channels 3 and 6 and extracellular signal-regulated kinases 1 and 2. Mol Cell Biol. 2013;33:4017-29 pubmed publisher
	García San Frutos M, Fernández Agulló T, Carrascosa J, Horrillo D, Barrús M, Oliveros E, et al. Involvement of protein tyrosine phosphatases and inflammation in hypothalamic insulin resistance associated with ageing: effect of caloric restriction. Mech Ageing Dev. 2012;133:489-97 pubmed publisher
	Wu Y, Wang N, Gu L, Yang H, Xia N, Zhang H. The suppressive effect of metabotropic glutamate receptor 5 (mGlu5) inhibition on hepatocarcinogenesis. Biochimie. 2012;94:2366-75 pubmed publisher
	Sun L, Gu L, Wang S, Yuan J, Yang H, Zhu J, et al. N-acetylcysteine protects against apoptosis through modulation of group I metabotropic glutamate receptor activity. PLoS ONE. 2012;7:e32503 pubmed publisher
	Trachtenberg A, Pushkarsky T, Heine S, Constant S, Brichacek B, Bukrinsky M. The level of CD147 expression correlates with cyclophilin-induced signalling and chemotaxis. BMC Res Notes. 2011;4:396 pubmed publisher
	Gangehei L, Ali M, Zhang W, Chen Z, Wakame K, Haidari M. Oligonol a low molecular weight polyphenol of lychee fruit extract inhibits proliferation of influenza virus by blocking reactive oxygen species-dependent ERK phosphorylation. Phytomedicine. 2010;17:1047-56 pubmed publisher

product information

Catalog Number :

05-797R

Subcategory :

Cell Signaling

Product Name :

Anti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P28482;P27361

Host Name :

Rabbit

Antigen :

phospho-Erk1/2 (Thr202/Tyr204

Clone :

AW39R

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonal

Cross Reactivity :

Human;Mouse;Rat

Background :

Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).

ALT Names :

Erk1;Erk2;p44-MAPK;p42-MAPK;MAPK2;PRKM2;p41mapk;PRKM1;p44;ERK2;P42MAPK;ERK;p42;MAPK1;ERT1;ERT2;Mitogen-activated protein kinase;MAP Kinase 2;MAP Kianse 1;MAPK

Immunogen :

Phosphorylated peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated

Specificity :

Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.

Package Size :

100 μL

Uses :

Multiplexing;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.