mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal | EMD Millipore 05-791R product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

catalog :

05-791R

quantity :

100 µL

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

AW304

reactivity :

human

application :

western blot

citations: 1

Reference
Kougioumtzi A, Tsaparas P, Magklara A. Deep sequencing reveals new aspects of progesterone receptor signaling in breast cancer cells. PLoS ONE. 2014;9:e98404 pubmed publisher

product information

Catalog Number :

05-791R

Subcategory :

Epigenetics & Nuclear Function

Product Name :

Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P68431

Host Name :

Rabbit

Antigen :

mono/di/trimethyl-Histone H3 (Lys4)

Clone :

AW304

Conjugate :

Purified

Isotype :

IgG

Product Description :

Anti-mono/di/trimethyl-Histone H3 (Lys4) Antibody, clone AW304, rabbit monoclonal

Cross Reactivity :

Human

Background :

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Histone methylation is primarily associated with heterochromatin and gene silencing.

ALT Names :

histone H3.1;histone H3/a;histone H3/b;histone H3/c;histone H3/d;histone H3/f;histone H3/h;histone H3/I;histone H3/j;histone H3/k;histone H3/l;histone 1, H3f;OTTHUMP00000016151;H3 histone family, member I

Immunogen :

KLH-conjugated linear peptide corresponding to human Histone H3 mono/di/tri-methylated at Lys4.

Specificity :

This antibody recognizes Histone H3 mono/di/tri-methylated at Lys4.

Package Size :

100 µL

Uses :

Western Blotting;Peptide Inhibition Assay

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.