Rho (-A Antibody, -B, -C), clone 55 | EMD Millipore 05-778 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

EMD Millipore

other brands :

Oncogene Research Products, Calbiochem, Novagen, Merck, Upstate Biotechnology, Chemicon, LINCO, Novabiochem, Guava

product type :

antibody

product name :

Rho (-A Antibody, -B, -C), clone 55

catalog :

05-778

quantity :

200 µg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:200; loading ...; fig s4g	Nishimura S, Mishra Gorur K, Park J, Surovtseva Y, Sebti S, Levchenko A, et al. Combined HMG-COA reductase and prenylation inhibition in treatment of CCM. Proc Natl Acad Sci U S A. 2017;114:5503-5508 pubmed publisher
western blot; human; 1:250; loading ...; fig 1b	Platet N, Hinkel I, Richert L, Murdamoothoo D, Moufok Sadoun A, Vanier M, et al. The tumor suppressor CDX2 opposes pro-metastatic biomechanical modifications of colon cancer cells through organization of the actin cytoskeleton. Cancer Lett. 2017;386:57-64 pubmed publisher
western blot; rat; 1:1000; fig 4	Lee M, Tsai K, Hsu J, Shin S, Wu J, Yeh J. Liraglutide prevents and reverses monocrotaline-induced pulmonary arterial hypertension by suppressing ET-1 and enhancing eNOS/sGC/PKG pathways. Sci Rep. 2016;6:31788 pubmed publisher
	Sycheva M, Sustarich J, Zhang Y, Selvaraju V, Geetha T, Gearing M, et al. Pro-Nerve Growth Factor Induces Activation of RhoA Kinase and Neuronal Cell Death. Brain Sci. 2019;9: pubmed publisher
	Tripathi B, Lowy D, Zelenka P. The Cdk5 activator P39 specifically links muskelin to myosin II and regulates stress fiber formation and actin organization in lens. Exp Cell Res. 2015;330:186-98 pubmed publisher
	Chen Y, Chu H, Ze Shiang Lin -, Shiah W, Chou C, Klimstra D, et al. p16 Stimulates CDC42-dependent migration of hepatocellular carcinoma cells. PLoS ONE. 2013;8:e69389 pubmed publisher
	Barateiro A, Miron V, Santos S, Relvas J, Fernandes A, Ffrench Constant C, et al. Unconjugated bilirubin restricts oligodendrocyte differentiation and axonal myelination. Mol Neurobiol. 2013;47:632-44 pubmed publisher
	Bordeleau F, Myrand Lapierre M, Sheng Y, Marceau N. Keratin 8/18 regulation of cell stiffness-extracellular matrix interplay through modulation of Rho-mediated actin cytoskeleton dynamics. PLoS ONE. 2012;7:e38780 pubmed publisher
	Croft D, Crighton D, Samuel M, Lourenco F, Munro J, Wood J, et al. p53-mediated transcriptional regulation and activation of the actin cytoskeleton regulatory RhoC to LIMK2 signaling pathway promotes cell survival. Cell Res. 2011;21:666-82 pubmed publisher
	Taiyab A, Rao C. HSP90 modulates actin dynamics: inhibition of HSP90 leads to decreased cell motility and impairs invasion. Biochim Biophys Acta. 2011;1813:213-21 pubmed publisher
	Tripathi B, Zelenka P. Cdk5-dependent regulation of Rho activity, cytoskeletal contraction, and epithelial cell migration via suppression of Src and p190RhoGAP. Mol Cell Biol. 2009;29:6488-99 pubmed publisher
	Zambuzzi W, Yano C, Cavagis A, Peppelenbosch M, Granjeiro J, Ferreira C. Ascorbate-induced osteoblast differentiation recruits distinct MMP-inhibitors: RECK and TIMP-2. Mol Cell Biochem. 2009;322:143-50 pubmed publisher

product information

Catalog Number :

05-778

Subcategory :

Cell Signaling

Product Name :

Anti-Rho (-A Antibody, -B, -C), clone 55

Product Type :

Antibodies

Clonality :

Monoclonal Antibody

Gene ID :

P61586

Host Name :

Mouse

Antigen :

Rho (-A

Clone :

Conjugate :

Purified

Isotype :

IgG1

Product Description :

Anti-Rho (-A Antibody, -B, -C), clone 55

Cross Reactivity :

Human;Mouse;Rat

Background :

Rho proteins belong to the Ras super family of low molecular weight GTPases, which includes RAS, RHO, RAB, RAN, RAD and ARF. These small GTP binding proteins function as molecular switches that regulate a multitude of biological processes including cell proliferation, apoptosis, differentiation, cytoskeletal reorganization, and membrane trafficking.

ALT Names :

Aplysia ras-related homolog 12;oncogene RHO H12;ras homolog gene family, member A;small GTP binding protein RhoA

Immunogen :

Recombinant human RhoA containing amino acids 1-155. Clone 55.

Specificity :

Recognizes Rho (-A, -B, -C), Mr 22 kDa.

Package Size :

200 µg

Uses :

Immunocytochemistry;Western Blotting

Storage :

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.